C-terminal substitution of HBV core proteins with those from DHBV reveals that Arginine-rich 167RRRSQSPRR175 domain is critical for HBV replication

PLoS ONE

Volumn 7, Issue 7, 2012, Pages

  Jung, Jaesung ^a   Kim, Hee Young ^a,b   Kim, Taeyeung ^a   Shin, Bo Hye ^a   Park, Gil Soon ^a,c   Park, Sun ^a   Chwae, Yong Joon ^a   Shin, Ho Joon ^a   Kim, Kyongmin ^a  

^a Ajou University School of Medicine   (South Korea)

^b Institute Pasteur Korea   (South Korea)

^c National Forensic Service   (South Korea)

Author keywords

[No Author keywords available]

Indexed keywords

ARGININE; CHIMERIC PROTEIN; CORE PROTEIN; GENOMIC RNA; PROTEIN VARIANT; VIRUS PROTEIN;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARTICLE; CARBOXY TERMINAL SEQUENCE; CHIMERA; CONTROLLED STUDY; DNA BINDING; DNA SYNTHESIS INHIBITION; DUCK HEPATITIS B VIRUS; NONHUMAN; PARTICLE SIZE; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN PHOSPHORYLATION; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY; STRUCTURE ANALYSIS; VIRUS REPLICATION;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ANIMALS; ARGININE; CELL LINE; DNA REPLICATION; DNA, VIRAL; HEPATITIS B VIRUS; HEPATITIS B VIRUS, DUCK; HUMANS; MOLECULAR SEQUENCE DATA; MUTANT CHIMERIC PROTEINS; PROTEIN BINDING; PROTEIN INTERACTION DOMAINS AND MOTIFS; RNA; RNA, VIRAL; VIRAL CORE PROTEINS; VIRUS REPLICATION;

DUCK HEPATITIS B VIRUS; HEPATITIS B VIRUS;

EID: 84864108829     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0041087     Document Type: Article

References (44)

1. Brechot C, (2004) Pathogenesis of hepatitis B virus-related hepatocellular carcinoma: old and new paradigms. Gastroenterology 127: S56-S61.
2. Zhou S, Standring DN, (1992) Hepatitis B virus capsid particles are assembled from core-protein dimer precursors. Proc. Natl. Acad. Sci. USA 89: 10046-10050.
3. Birnbaum F, Nassal M, (1990) Hepatitis B virus nucleocapsid assembly: primary structure requirements in the core protein. J. Virol. 64: 3319-3330.
4. Gallina A, Bonelli F, Zentilin L, Rindi G, Muttini M, et al. (1989) A recombinant hepatitis B core antigen polypeptide with the protamine-like domain deleted self-assembles into capsid particles but fails to bind nucleic acid. J. Virol. 63: 4645-4652.
5. Beames B, Lanford RE, (1993) Carboxy-terminal truncations of the HBV core protein affect capsid formation and the apparent size of encapsidated HBV RNA. Virology 194: 597-607.
6. Köck J, Nassal M, Deres K, Blum HE, von Weizsäcker F, (2004) Hepatitis B virus nucleocapsids formed by carboxy-terminally mutated core proteins contain spliced viral genomes but lack full-size DNA. J. Virol. 78: 13812-13818.
7. Lewellyn EB, Loeb DD, (2011a) The arginine clusters of the carboxy-terminal domain of the core protein of hepatitis B virus make pleiotropic contributions to genome replication. J. Virol. 85: 1298-1309.
8. Lewellyn EB, Loeb DD, (2011b) Serine phosphoacceptor sites within the core protein of hepatitis B virus contribute to genome replication pleiotropically. PLoS One 6: e17202.
9. Le Pogam S, Chua PK, Newman M, Shih C, (2005) Exposure of RNA templates and encapsidation of spliced viral RNA are influenced by the arginine-rich domain of human hepatitis B virus core antigen (HBcAg 165-173). J. Virol. 79: 1871-1887.
10. Nassal M, (1992) The arginine-rich domain of the hepatitis B virus core protein is required for pregenome encapsidation and productive viral positive-strand DNA synthesis but not for virus assembly. J. Virol. 66: 4107-4116.
11. Berkowitz RD, Ohagen A, Höglund S, Goff SP, (1995) Retroviral nucleocapsid domains mediate the specific recognition of genomic viral RNAs by chimeric gag polyproteins during RNA packaging in vivo. J. Virol. 69: 6445-6456.
12. Certo JL, Kabdulov IO, Paulson ML, Anderson JA, Hu WS, (1999) The nucleocapsid domain is responsible for the ability of spleen nerosis virus (SNV) gag polyprotein to package both SNV and murine leukemia virus RNA. J. Virol. 73: 9170-9177.
13. Kaye JF, Lever AML, (1998) Nonreciprocal packaging of human immunodeficiency virus type 1 and type 2 RNA: a possible role for the p2 domain of gag in RNA encapsidation. J. Virol (72): 5877-5885.
14. Kim KH, Strauss EG, Strauss JH, (2000) Adaptive mutations in Sindbis virus E2 and Ross River virus E1 that allow efficient budding of chimeric viruses. J. Virol. 74: 2663-2670.
15. Mueller-Hill K, Loeb DD, (2002) cis-acting sequences 5E, M, and 3E interact to contribute to primer translocation and circularization during reverse transcription of avian hepadnavirus DNA. J. Virol. 76: 4260-4266.
16. Ostrow KM, Loeb DD, (2002) Characterization of the cis-acting contributions to avian hepadnavirus RNA encapsidation. J. Virol. 76: 9087-9095.
17. Ostrow KM, Loeb DD, (2004) Chimeras of duck and heron hepatitis B viruses provide evidence for functional interactions between viral components of pregenomic RNA encapsidation. J. Virol. 78: 8780-8787.
18. Kodama K, Ogasawara N, Yoshikawa H, Murakami S, (1985) Nucleotide sequence of a cloned woodchuck hepatitis virus genome: evolutional relationship between hepadnaviruses. J. Virol. 56: 978 986.
19. Mandart E, Kay A, Galibert F, (1984) Nucleotide sequence of a cloned duck hepatitis B virus genome: Comparison with woodchuck and human hepatitis B virus sequences. J. Virol. 49: 782-792.
20. Okamoto H, Omi S, Wang Y, Imai M, Mayum M, (1990) Trans-complementation of the C gene of human and the P gene of woodchuck hepadnaviruses. J. Gen. Virol. 71: 959-963.
21. Zierman R, Ganem D, (1996) Homologous and heterologous complementation of HBV and WHV capsid and polymerase functions in RNA encapsidation. Virology 219: 350-356.
22. Kenney JM, von Bonsdorff CH, Nassal M, Fuller SD, (1995) Evolutionary conservation in the hepatitis B virus core structure: comparison of human and duck cores. Structure 3: 1009-1019.
23. König S, Beterams G, Nassal M, (1998) Mapping of homologous interaction sites in the hepatitis B virus core protein. J. Virol. 72: 4997-5005.
24. Huang J, Liang TJ, (1993) A novel hepatitis B virus (HBV) genetic element with Rev response element-like properties that is essential for expression of HBV gene products. Mol. Cell. Biol. 13: 7476-7486.
25. Newman M, Suk FM, Cajimat M, Chua PK, Shih C, (2003) Stability and morphology comparisons of self-assembled virus-like particles from wild-type and mutant human hepatitis B virus capsid proteins. J. Virol. 77: 12950-12960.
26. Chua PK, Tang FM, Huang JY, Suen CS, Shih C, (2011) Testing the balanced electrostatic interaction hypothesis of hepatitis B virus DNA synthesis by using an in vivo charge rebalance approach. J.Virol. 84: 2340-2351.
27. Kim H-Y, Park G-S, Kim E-G, Kang S-H, Shin H-J, et al. (2004) Oligomer synthesis by priming deficient polymerase in hepatitis B virus core particle. Virology 322: 22-30.
28. Hatton T, Zhou S, Standring DN, (1992) RNA- and DNA-binding activities in hepatitis B virus capsid protein: a model for their roles in viral replication. J. Virol. 66: 5232-5241.
29. Park G-S, Kim H-Y, Shin H-S, Park S, Shin H-J, et al. (2008) Modulation of hepatitis B virus replication by expression of polymerase-surface fusion protein through splicing: implications for viral persistence. Virus Res. 136: 166-174.
30. Günther S, Sommer G, Iwanska A, Will H, (1997) Heterogeneity and common features of defective hepatitis B virus genomes derived from spliced pregenomic RNA. Virology. 238: 363-371.
31. Köck J, Wieland S, Blum HE, von Weizsäcker F, (1998) Duck hepatitis B virus nucleocapsids formed by N-terminally extended or C-terminally truncated core proteins disintegrate during viral DNA maturation. J. Virol. 72: 9116-9120.
32. Gazina EV, Fielding JE, Lin B, Anderson DA, (2000) Core protein phosphorylation modulates pregenomic RNA encapsidation to different extents in human and duck hepatitis B virus. J. Virol. 74: 4721-4728.
33. Lan YT, Li J, Liao W, Ou J, (1999) Roles of the three major phosphorylation sites of hepatitis B virus core protein in viral replication. Virology 259: 342-348.
34. Liao W, Ou JH, (1995) Phosphorylation and nuclear localization of the hepatitis B virus core protein: significance of serine in the three repeated SPRRR motifs. J. Virol. 69: 1025-1029.
35. Machida A, Ohnuma H, Tsuda F, Yoshikawa A, Hashi Y, et al. (1991) Phosphorylation in the carboxyl-terminal domain of the capsid protein of hepatitis B virus: evaluation with a monoclonal antibody. J. Virol. 65: 6024-6030.
36. Yeh C-T, Ou JH, (1991) Phosphorylation of hepatitis B virus precore and core proteins. J. Virol. 65: 2327-2331.
37. Perlman DH, Berg EA, O'Connor PB, Costello CE, Hu J, (2005) Reverse transcription-associated dephosphorylation of hepadnavirus nucleocapsids. Proc. Natl. Acad. Sci. USA 102: 9020-9025.
38. Yu M, Summers J, (1994) Phosphorylation of the duck hepatitis B virus capsid protein associated with conformational changes in the C terminus. J. Virol. 68: 2965-2969.
39. Kann M, Gerlich WH, (1994) Effect of core protein phosphorylation by protein kinase C on encapsidation of RNA within core particles of hepatitis B virus. J. Virol. 68: 7993-8000.
40. Yeh CT, Wong SW, Fung YK, Ou JH, (1993) Cell cycle regulation of nuclear localization of hepatitis B virus core protein. Proc. Natl. Acad. Sci. USA 90: 6459-6463.
41. Kau JH, Ting LP, (1998) Phosphorylation of the core protein of hepatitis B virus by a 46-kilodalton serine kinase. J. Virol. 72: 3796-3803.
42. Daub H, Blencke S, Habenberger P, Kurtenbach A, Dennenmoser J, et al. (2002) Identification of SRPK1 and SRPK2 as the major cellular protein kinases phosphorylating hepatitis B virus core protein. J. Virol. 76: 8124-8137.
43. Melegari M, Wolf SK, Schneider RJ, (2005) Hepatitis B virus DNA replication is coordinated by core protein serine phosphorylation and HBx expression. J. Virol. 79: 9810-9820.
44. Enomoto M, Sawano Y, Kosuge S, Yamano Y, Kuroki K, et al. (2006) High phosphorylation of HBV core protein by two a-type CK2-activated cAMP-dependent protein kinases in vitro. FEBS Lett. 580: 894-899.