Core protein phosphorylation modulates pregenomic RNA encapsidation to different extents in human and duck hepatitis B viruses

Journal of Virology

Volumn 74, Issue 10, 2000, Pages 4721-4728

  Gazina, Elena V ^a,b   Fielding, James E ^a   Lin, Bo ^a   Anderson, David A ^a  

^a MACFARLANE BURNET INSTITUTE FOR MEDICAL RESEARCH AND PUBLIC HEALTH   (Australia)

^b D I IVANOVSKY INSTITUTE OF VIROLOGY   (Russian Federation)

Author keywords

[No Author keywords available]

Indexed keywords

ALANINE; ASPARTIC ACID; CORE PROTEIN; DIMER; PHOSPHOSERINE; SERINE; VIRUS DNA; VIRUS RNA;

AMINO ACID SUBSTITUTION; ARTICLE; CONTROLLED STUDY; DNA REPLICATION; DNA SYNTHESIS; HEPATITIS B VIRUS; HUMAN; HUMAN CELL; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; PROTEIN VARIANT; VIRUS CAPSID;

AMINO ACID SEQUENCE; ANIMALS; CAPSID; CELL LINE; GENOME, VIRAL; HEPATITIS B VIRUS; HEPATITIS B VIRUS, DUCK; HUMANS; MOLECULAR SEQUENCE DATA; MUTATION; PHOSPHORYLATION; PLASMIDS; RNA PRECURSORS; RNA, VIRAL; TRANSFECTION; VIRAL CORE PROTEINS;

EID: 0033999564     PISSN: 0022538X     EISSN: None     Source Type: Journal    
DOI: 10.1128/JVI.74.10.4721-4728.2000     Document Type: Article

References (22)

1. Bartenschlager, R., M. Junker-Niepmann, and H. Schaller. 1990. The P gene product of hepatitis B virus is required as a structural component for genomic RNA encapsidation. J. Virol. 64:5324-5332.
2. Bearnes, B., and R. E. Lanford. 1993. Carboxy-terminal truncations of the HBV core protein affect capsid formation and the apparent size of encapsidated HBV RNA. Virology 194:597-607.
3. Galibert, F., E. Mandart, F. Fitoussi, P. Tiollais, and P. Charnay. 1979. Nudeotide sequence of the hepatitis B virus genome (subtype ayw) cloned in E. coli. Nature 281:646-650.
4. Ganem, D. 1996. Hepadnaviridae: the viruses and their replication, p. 2703-2737. In B. Fields, D. M. Knipe, and P. M. Howley (ed.), Virology. Raven Publishers, Philadelphia, Pa.
5. Gorman, C. 1985. High efficiency gene transfer into mammalian cells, p. 143-190. In D. M. Glover (ed.), DNA cloning: a practical approach. IRL Press, Oxford, United Kingdom.
6. Hirsch, R. C., J. E. Lavine, L. Chang, H. E. Varmus, and D. Ganem. 1990. Polymerase gene products of hepatitis B viruses are required for genomic RNA packaging as well as for reverse transcription. Nature 344:552-555.
7. Kann, M., and W. H. Gerlich. 1994. Effect of core protein phosphorylation by protein kinase C on encapsidation of RNA within core particles of hepatitis B virus. J. Virol. 68:7993-8000.
8. Lan, Y. T., J. Li, W.-Y. Liao, and J.-H. Ou. 1998. Roles of the three major phosphorylation sites of hepatitis B virus core protein in viral replication. Virology 259:342-348.
9. Lanford, R. E., and L. Notvall. 1990. Expression of hepatitis B virus core and precore antigens in insect cells and characterization of a core-associated kinase activity. Virology 176:222-233.
10. Liao, W., and J.-H. Ou. 1995. Phosphorylation and nuclear localization of the hepatitis B virus core protein: significance of serine in the three repeated SPRRR motifs. J. Virol. 69:1025-1029.
11. Machida, A., H. Ohnuma, F. Tsuda, A. Yoshikawa, Y. Hoshi, T. Tanaka, S. Kishimoto, Y. Akahane, Y. Miyakawa, and M. Mayumi. 1991. Phosphorylation in the carboxyl-terminal domain of the capsid protein of hepatitis B virus: evaluation with a monoclonal antibody. J. Virol. 65:6024-6030.
12. Mandart, E., A. Kay, and F. Galibert. 1984. Nucleotide sequence of a cloned duck hepatitis B virus genome: comparison with woodchuck and human hepatitis B virus sequences. J. Virol. 49:782-792.
13. Nassal, M. 1992. The arginine-rich domain of the hepatitis B virus core protein is required for pregenome encapsidation and productive viral positive-strand DNA synthesis but not for virus assembly. J. Virol. 66:4107-4116.
14. Pugh, J., A. Zweidler, and J. Summers. 1989. Characterization of the major duck hepatitis B virus core particle protein. J. Virol. 63:1371-1376.
15. Roossinck, M. J., and A. Siddiqui. 1987. In vivo phosphorylation and protein analysis of hepatitis B virus core antigen. J. Virol. 61:955-961.
16. Scaglioni, P. P., M. Melegari, and J. R. Wands. 1997. Posttranscriptional regulation of hepatitis B virus replication by the precore protein. J. Virol. 71:345-353.
17. Schlicht, H. J., R. Batrenschlager, and H. Schaller. 1989. The duck hepatitis B virus core protein contains a highly phosphorylated C terminus that is essential for replication but not for RNA packaging. J. Virol. 63:2995-3000.
18. Summers, J., P. M. Smith, M. J. Huang, and M. S. Yu. 1991. Morphogenetic and regulatory effects of mutations in the envelope proteins of an avian hepadnavirus. J. Virol. 65:1310-1317.
19. von Weizsacker, F., J. Kock, S. Wieland, W.-B. Offensperger, and H. E. Blum. 1999. Dominant negative mutants of the duck hepatitis B virus core protein interfere with RNA pregenome packaging and viral DNA synthesis. Hepatology 30:308-315.
20. Yu, M., and J. Summers. 1994. Phosphorylation of the duck hepatitis B virus capsid protein associated with conformational changes in the C terminus. J. Virol. 68:2965-2969.
21. Yu, M., and J. Summers. 1994. Multiple functions of capsid protein phosphorylation in duck hepatitis B virus replication. J. Virol. 68:4341-4348.
22. Zhou, S., and D. N. Standring. 1992. Hepatitis B virus capsid particles are assembled from core-protein dimer precursors. Proc. Natl. Acad. Sci. USA 89:10046-10050.