Modulation of hepatitis B virus replication by expression of polymerase-surface fusion protein through splicing: Implications for viral persistence

Virus Research

Volumn 136, Issue 1-2, 2008, Pages 166-174

  Park, Gil Soon ^a   Kim, Hee Young ^a   Shin, Hyun Soo ^a   Park, Sun ^a   Shin, Ho Joon ^a   Kim, Kyongmin ^a  

^a Ajou University School of Medicine   (South Korea)

Author keywords

Hepatitis B virus replication; Polymerase surface fusion protein; Spliced transcript

Indexed keywords

DNA DIRECTED DNA POLYMERASE ALPHA; HEPATITIS B SURFACE ANTIGEN; HYBRID PROTEIN; VIMENTIN;

ARTICLE; ENDOPLASMIC RETICULUM; GENE EXPRESSION; GENETIC TRANSCRIPTION; HEPATITIS B VIRUS; MICROTUBULE; PERSISTENT VIRUS INFECTION; PRIORITY JOURNAL; RNA SPLICING; VIRUS REPLICATION;

CELL LINE; CYTOPLASM; ENDOPLASMIC RETICULUM; GENE PRODUCTS, POL; HEPATITIS B SURFACE ANTIGENS; HEPATITIS B VIRUS; HEPATOCYTES; HUMANS; MICROTUBULES; RNA SPLICING; VIMENTIN; VIRAL FUSION PROTEINS; VIRUS ASSEMBLY; VIRUS REPLICATION;

HEPATITIS B VIRUS;

EID: 46049101970     PISSN: 01681702     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.virusres.2008.05.005     Document Type: Article

References (33)

1. Bartenschlager R., Junker-Niepmann M., and Schaller H. The P gene product of hepatitis B virus is required as a structural component for genomic RNA encapsidation. J. Virol. 64 (1990) 5324-5332
2. Bartenschlager R., and Schaller H. Hepadnaviral assembly is initiated by polymerase binding to the encapsidation signal in the viral RNA genome. EMBO J. 11 (1992) 3413-3420
3. Brechot C. Pathogenesis of hepatitis B virus-related hepatocellular carcinoma: old and new paradigms. Gastroenterology 127 (2004) S56-S61
4. Cao F., and Tavis J.E. Detection and characterization of cytoplasmic hepatitis B virus reverse transcriptase. J. Gen. Virol. 85 (2004) 3353-3360
5. Chen P.J., Chen C.R., Sung J.L., and Chen D.S. Identification of a doubly spliced viral transcript joining the separated domains for putative protease and reverse transcriptase of hepatitis B virus. J. Virol. 63 (1989) 4165-4171
6. Chisari F.V., Filippi P., McLachlan A., Milich D.R., Riggs M., Lee S., Palmiter R.D., Pinkert C.A., and Brinster R.L. Expression of hepatitis B virus large envelope polypeptide inhibits hepatitis B surface antigen secretion in transgenic mice. J. Virol. 60 (1986) 880-887
7. Chisari F.V., Filippi P., Buras J., McLachlan A., Popper H., Pinkert C.A., Palmiter R.D., and Brinster R.L. Structural and pathological effects of synthesis of hepatitis B virus large envelope polypeptide in transgenic mice. Proc. Natl. Acad. Sci. U.S.A. 84 (1987) 6909-6913
8. Choo K.B., Liew L.N., Chong K.Y., Lu R.H., and Cheng W.T. Transgenome transcription and replication in the liver and extrahepatic tissues of a human hepatitis B virus transgenic mouse. Virology 182 (1991) 785-792
9. Edman J.C., Gray P., Valenzuela P., Rall L.B., and Rutter W.J. Intergration of hepatitis B virus sequences and their expression in a human hepatoma cell. Nature 286 (1980) 535-538
10. Ganem D., and Schneider R.J. Hepadnaviridae: the Viruses and their replication. In: Knipe D.M., and Howley P.M. (Eds). Fields Virology. 4th ed. (2001), Lippincott Williams and Wilkins, Philadelphia 2923-2969
11. Gerken G., Kremsdorf D., Capel F., Petit M.A., Dauguet C., Manns M.P., Meyer Zum Buschenfelde K.H., and Brechot C. Hepatitis B defective virus with rearrangement in the Pre S gene during chronic HBV infection. Virology 183 (1991) 555-565
12. Guan K.L., and Dixon J.E. Eukaryotic proteins expressed in Escherichia coli: an improved thrombin cleavage and purification procedure of fusion proteins with glutathione S-transferase. Anal. Biochem. 192 (1991) 262-267
13. Gunther S., Sommer G., Iwanska A., and Will H. Heterogeneity and common features of defective hepatitis B virus genomes derived from spliced pregenomic RNA. Virology 238 (1997) 363-371
14. Hass M., Hannoun C., Kalinina T., Sommer G., Manegold C., and Gunther S. Functional analysis of hepatitis B virus reactiviating in hepatitis B surface antigen-negative individuals. Hepatology 42 (2005) 93-103
15. Hirsch R.C., Lavine J.E., Chang L.J., Varmus H.E., and Ganem D. Polymerase gene products of hepatitis B viruses are required for genomic RNA packaging as well as for reverse transcription. Nature 344 (1990) 552-555
16. Huang H.L., Jeng K.S., Hu C.P., Tsai C.H., Lo S.J., and Chang C. Identification and characterization of a structural protein of hepatitis B virus: a polymerase and surface fusion protein encoded by a spliced RNA. Virology 275 (2000) 398-410
17. Kim H.Y., Park G.S., Kim E.G., Kang S.H., Shin H.J., Park S., and Kim K.H. Oligomer synthesis by priming deficient polymerase in hepatitis B virus core particle. Virology 322 (2004) 22-30
18. Koschel M., Oed D., Gerelsaikhan T., Thomssen R., and Bruss V. Hepatitis B virus core gene mutations which block nucleocapsid envelopment. J. Virol. 74 (2000) 1-7
19. Kuroki K., Russnak R., and Ganem D. Novel N-terminal amino acid sequence required for retention of a hepatitis B virus glycoprotein in the endoplasmic reticulum. Mol. Cell. Biol. 9 (1989) 4459-4466
20. Lu Y.W., Tan T.L., Chan V., and Chen W.N. The HBSP gene is expressed during HBV replication, and its coded BH3-containing spliced viral protein induces apoptosis in HepG2 cells. Biochem. Biophys. Res. Commun. 351 (2006) 64-70
21. Miller R.H., Girones R., Cote P.J., Hornbuckle W.E., Chestnut T., Baldwin B.H., Korba B.E., Tennant B.C., Gerin J.L., and Purcell R.H. Evidence against a requisite role for defective virus in the establishment of persistent hrpadnavirus infections. Proc. Natl. Acad. Sci. U.S.A. 87 (1990) 9329-9332
22. Nemeckova S., Kunke D., Press M., Nemecek V., and Kutinova L. A carboxy-terminal portion of the preS1 domain of hepatitis B virus (HBV) occasioned retention in endoplasmic reticulum of HBV envelope proteins expressed by recombinant vaccinia viruses. Virology 202 (1994) 1024-1027
23. Obert S., Zachmann-Brand B., Deindl E., Tucker W., Bartenschlager R., and Schaller H. A splice hepadnavirus RNA that is essential for virus replication. EMBO J. 15 (1996) 2565-2574
24. Ou J.H., and Rutter W.J. Regulation of secretion of the hepatitis B virus major surface antigen by the preS-1 protein. J. Virol. 61 (1987) 782-786
25. Radziwill G., Tucker W., and Schaller H. Mutational analysis of the hepatitis B virus P gene product: domain structure and RNase H activity. J. Virol. 64 (1990) 613-620
26. Rosmorduc O., Petit M.A., Pol S., Capel F., Bortolotti F., Berthelot P., Brechot C., and Kremsdorf D. In vivo and in vitro expression of defective hepatitis B virus particles generated by spliced hepatitis B virus RNA. Hepatology 22 (1995) 10-19
27. Soussan P., Garreau F., Zylberberg H., Ferray C., Brechot C., and Kremsdorf D. In vivo expression of a new hepatitis B virus protein encoded by a spliced RNA. J. Clin. Invest. 105 (2000) 55-60
28. Soussan P., Tuveri R., Nalpas B., Garreau F., Zavala F., Masson A., Pol S., Brechot C., and Kremsdorf D. The expression of hepatitis B spliced protein (HBSP) encoded by a spliced hepatitis B virus RNA is associated with viral replication and liver fibrosis. J. Hepatol. 38 (2003) 343-348
29. Su T.S., Lai C.J., Huang J.L., Lin L.H., Yauk Y.K., Chang C.M., Lo S.J., and Han S.H. Hepatitis B virus transcript produced by RNA splicing. J. Virol. 63 (1989) 4011-4018
30. Terre S., Petit M.A., and Brechot C. Defective hepatitis B virus particles are generated by packaging and reverse transcription of spliced viral RNAs in vivo. J. Virol. 65 (1991) 5539-5543
31. Wu H.L., Chen P.J., Tu S.J., Lin M.H., Lai M.Y., and Chen D.S. Characterization and genetic analysis of alternatively spliced transcripts of hepatitis B virus in infected human liver tissues and transfected HepG2 cells. J. Virol. 65 (1991) 1680-1686
32. Ziemer M., Garcis P., Shaul Y., and Rutter W.J. Sequence of hepatitis B virus DNA incorporated into the genome of a human hepatoma cell line. J. Virol. 53 (1985) 885-892
33. zu Putlitz J., Lanford R.E., Carlson R.I., Notvall L., de la Monte S.M., and Wands J.R. Properties of monoclonal antibodies directed against hepatitis B virus polymerase protein. J. Virol. 73 (1999) 4188-4196