Simple absolute quantification method correcting for quantitative PCR efficiency variations for microbial community samples

Applied and Environmental Microbiology

Volumn 78, Issue 12, 2012, Pages 4481-4489

  Brankatschk, Robert ^a   Bodenhausen, Natacha ^a   Zeyer, Josef ^a   Burgmann, Helmut ^b  

^a ETH ZURICH   (Switzerland)

^b SWISS FEDERAL INSTITUTE OF AQUATIC SCIENCE AND TECHNOLOGY   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

16S RRNA GENE; ABSOLUTE QUANTIFICATION; ALGAE AND PROTOZOON; BACTERIAL STRAINS; COPY NUMBER; CULTURE COLLECTION; ENVIRONMENTAL SAMPLE; GENE COPY NUMBER; GEOBACTER SULFURREDUCENS; MICROBIAL COMMUNITIES; MICROBIAL COMMUNITY ANALYSIS; ORDERS OF MAGNITUDE; QUANTITATIVE PCR; REAL-TIME QUANTITATIVE PCR; RELATIVE QUANTIFICATION; TARGET GENES;

GENE EXPRESSION; MICROORGANISMS; RNA;

POLYMERASE CHAIN REACTION;

NITROGENASE REDUCTASE; OXIDOREDUCTASE; RNA 16S;

BACTERIUM; CALIBRATION; GENE EXPRESSION; MICROBIAL COMMUNITY; POLYMERASE CHAIN REACTION; PROTOZOAN; QUANTITATIVE ANALYSIS; REAL TIME;

ARTICLE; BACTERIAL LOAD; EVALUATION; GENETICS; GEOBACTER; ISOLATION AND PURIFICATION; METHODOLOGY; MICROBIOLOGY; NOSTOC COMMUNE; REAL TIME POLYMERASE CHAIN REACTION; STANDARD;

BACTERIAL LOAD; ENVIRONMENTAL MICROBIOLOGY; GEOBACTER; NOSTOC COMMUNE; OXIDOREDUCTASES; REAL-TIME POLYMERASE CHAIN REACTION; RNA, RIBOSOMAL, 16S;

ALGAE; BACTERIA (MICROORGANISMS); GEOBACTER SULFURREDUCENS; NOSTOC COMMUNE; PROTOZOA;

EID: 84864100140     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.07878-11     Document Type: Article

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