Reevaluation and reduction of a PCR bias caused by reannealing of templates

Applied and Environmental Microbiology

Volumn 70, Issue 12, 2004, Pages 7545-7549

  Kurata, Shinya ^a,b,c   Kanagawa, Takahiro ^b,c   Magariyama, Yukio ^d   Takatsu, Kyoko ^a,b   Yamada, Kazutaka ^a,b   Yokomaku, Toyokazu ^a   Kamagata, Yoichi ^b  

^a Kankyo Engineering Co Ltd   (Japan)

^b NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY AIST   (Japan)

^c TOKYO INSTITUTE OF TECHNOLOGY   (Japan)

^d NATIONAL FOOD RESEARCH INSTITUTE   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

ANNEALING; ECOLOGY; ENVIRONMENTAL IMPACT; REDUCTION;

DENATURATION; PCR; TEMPLATES;

MICROBIOLOGY;

BACTERIAL RNA; DOUBLE STRANDED RNA; HETERODUPLEX; RNA 16S;

MICROBIAL ECOLOGY; POLYMERASE CHAIN REACTION; SAMPLING BIAS;

ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; DENATURATION; DNA TEMPLATE; ECOLOGY; GENE AMPLIFICATION; NONHUMAN; REAL TIME POLYMERASE CHAIN REACTION; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; RNA STRUCTURE; SEQUENCE HOMOLOGY; STRUCTURE ANALYSIS; TEMPERATURE DEPENDENCE; THERMOSTABILITY;

ACTINOMYCETALES; BIAS (EPIDEMIOLOGY); NUCLEIC ACID DENATURATION; NUCLEIC ACID HYBRIDIZATION; POLYMERASE CHAIN REACTION; PSEUDOMONAS FLUORESCENS; RNA, RIBOSOMAL, 16S; TEMPERATURE; TEMPLATES, GENETIC;

BACTERIA (MICROORGANISMS);

EID: 10444268966     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.70.12.7545-7549.2004     Document Type: Article

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