A blue native polyacrylamide gel electrophoretic technology to probe the functional proteomics mediating nitrogen homeostasis in Pseudomonas fluorescens

Journal of Microbiological Methods

Volumn 90, Issue 3, 2012, Pages 206-210

  Han, Sungwon ^a   Auger, Christopher ^a   Appanna, Varun P ^a   Lemire, Joseph ^a   Castonguay, Zachary ^a   Akbarov, Elchin ^b   Appanna, Vasu D ^a  

^a LAURENTIAN UNIVERSITY   (Canada)

^b KHAZAR UNIVERSITY   (Azerbaijan)

Author keywords

Functional proteomics; Gel electrophoresis; Glutamate dehydrogenase; Nitrogen homeostasis

Indexed keywords

2,6 DICHLOROPHENOLINDOPHENOL; ALANINE AMINOTRANSFERASE; AMMONIA; ASPARTATE AMINOTRANSFERASE; CARBAMOYL PHOSPHATE SYNTHASE; GLUTAMATE DEHYDROGENASE; GLUTAMIC ACID; GLUTAMINASE; GLYCINE AMINOTRANSFERASE; IODONITROTETRAZOLIUM; NITROGEN; ORNITHINE OXOACID AMINOTRANSFERASE; PHENAZINE METHOSULFATE; TETRAZOLIUM; UNCLASSIFIED DRUG;

ARTICLE; CONCENTRATION (PARAMETERS); ENZYME ACTIVITY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HOMEOSTASIS; INCUBATION TIME; MOLECULAR PROBE; NITROGEN HOMEOSTASIS; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN FUNCTION; PROTEIN INTERACTION; PROTEOMICS; PSEUDOMONAS FLUORESCENS;

2,6-DICHLOROINDOPHENOL; ALANINE TRANSAMINASE; AMMONIA; ASPARTATE AMINOTRANSFERASES; BACTERIAL PROTEINS; CARBAMOYL-PHOSPHATE SYNTHASE (AMMONIA); ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME ASSAYS; GLUTAMATE DEHYDROGENASE; GLUTAMIC ACID; GLUTAMINASE; GLYCINE TRANSAMINASE; HOMEOSTASIS; METHYLPHENAZONIUM METHOSULFATE; NITROGEN; ORNITHINE-OXO-ACID TRANSAMINASE; PROTEOMICS; PSEUDOMONAS FLUORESCENS; TETRAZOLIUM SALTS;

PSEUDOMONAS FLUORESCENS;

EID: 84864093517     PISSN: 01677012     EISSN: 18728359     Source Type: Journal    
DOI: 10.1016/j.mimet.2012.05.006     Document Type: Article

References (34)

1. Abe T., Takenaka O., Inada Y. Reinvestigation of the physicochemical and enzymic properties of l-glutaminase from Pseudomonas. Biochim. Biophys. Acta - Enzymol. 1974, 358:113-116.
2. Ahuja A., Purcarea C., Guy H.I., Evans D.R. A novel carbamoyl-phosphate synthetase from Aquifex aeolicus. J. Biol. Chem. 2001, 276:45694-45703.
3. Amon J., Titgemeyer F., Burkovski A. A genomic view on nitrogen metabolism and nitrogen control in mycobacteria. J. Mol. Microbiol. Biotechnol. 2009, 17:20-29.
4. Auger C., Lemire J., Cecchini D., Bignucolo A., Appanna V.D. The metabolic reprogramming evoked by nitrosative stress triggers the anaerobic utilization of citrate in Pseudomonas fluorescens. PLoS One 2011, 6:e28469.
5. Auger C., Appanna V., Castonguay Z., Han S., Appanna V.D. A facile electrophoretic technique to monitor phosphoenolpyruvate-dependent kinases. Electrophoresis 2012, 33:1095-1101.
6. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 1976, 72:248-254.
7. Brosnan M.E., Brosnan J.T. Hepatic glutamate metabolism: a tale of 2 hepatocytes. Am. J. Clin. Nutr. 2009, 90:857S-861S.
8. Coleman P.F., Suttle D.P., Stark G.R. Purification from hamster cells of the multifunctional protein that initiates de novo synthesis of pyrimidine nucleotides. J. Biol. Chem. 1977, 252:6379-6385.
9. Duane L.P. A rapid colorimetric assay for carbamyl phosphate synthetase I. J. Biochem. Biophys. Methods 1980, 3:31-37.
10. Funck D., Stadelhofer B., Koch W. Ornithine-delta-aminotransferase is essential for arginine catabolism but not for proline biosynthesis. BMC Plant Biol. 2008, 8:40.
11. Gottlieb M., Chavko M. Silver staining of native and denatured eucaryotic DNA in agarose gels. Anal. Biochem. 1987, 165:33-37.
12. Hu W., Zhang C., Wu R., Sun Y., Levine A., Feng Z. Glutaminase 2, a novel p53 target gene regulating energy metabolism and antioxidant function. Proc. Natl. Acad. Sci. U. S. A. 2010, 107:7455-7460.
13. Kim H.R., Rho H.W., Park J.W., Park B.H., Kim J.S., Lee M.W. Assay of ornithine aminotransferase with ninhydrin. Anal. Biochem. 1994, 223:205-207.
14. Koo B., Hyun H., Kim S., Kim C., Lee H. Enhancement of thymidine production in E. coli by eliminating repressors regulating the carbamoyl phosphate synthetase operon. Biotechnol. Lett. 2011, 33:71-78.
15. Lain-Guelbenzu B., CÁRdenas J., MuNOz-Blanco J. Purification and properties of l-alanine aminotransferase from Chlamydomonas reinhardtii. Eur. J. Biochem. 1991, 202:881-887.
16. Lemire J., Milandu Y., Auger C., Bignucolo A., Appanna V.P., Appanna V.D. Histidine is a source of the antioxidant, α-ketoglutarate, in Pseudomonas fluorescens challenged by oxidative stress. FEMS Microbiol. Lett. 2010, 309:170-177.
17. Maddalo G., Chovanec P., Stenberg-Bruzell F., Nielsen H.V., Jensen-Seaman M.I., Ilag L.L., et al. A reference map of the membrane proteome of Enterococcus faecalis. Proteomics 2011, 11:3935-3941.
18. Mailloux R.J., Darwich R., Lemire J., Appanna V. The monitoring of nucleotide diphosphate kinase activity by blue native polyacrylamide gel electrophoresis. Electrophoresis 2008, 29:1484-1489.
19. Mally M.I., Grayson D.R., Evans D.R. Controlled proteolysis of the multifunctional protein that initiates pyrimidine biosynthesis in mammalian cells: evidence for discrete structural domains. Proc. Natl. Acad. Sci. U. S. A. 1981, 78:6647-6651.
20. Miller S.M., Magasanik B. Role of NAD-linked glutamate dehydrogenase in nitrogen metabolism in Saccharomyces cerevisiae. J. Bacteriol. 1990, 172:4927-4935.
21. Painter H.A. A review of literature on inorganic nitrogen metabolism in microorganisms. Water Res. 1970, 4:393-450.
22. Panakanti R., Pratap A., Yang N., Jackson J.S., Mahato R.I. Triplex forming oligonucleotides against type α1(I) collagen attenuates liver fibrosis induced by bile duct ligation. Biochem. Pharmacol. 2010, 80:1718-1726.
23. Prusiner S., Davis J.N., Stadtman E.R. Regulation of glutaminase B in Escherichia coli. I. Purification, properties, and cold lability. J. Biol. Chem. 1976, 251:3447-3456.
24. Reinoso C.A., Auger C., Appanna V.D., Vásquez C.C. Tellurite-exposed Escherichia coli exhibits increased intracellular α-ketoglutarate. Biochem. Biophys. Res. Commun. 2012, 10.1016/j.bbrc.2012.04.069.
25. Schagger, von Jagow G. Blue native electrophoresis for isolation of membrane protein complexes in enzymatically active form. Anal. Biochem. 1991, 199:223-231.
26. Singh R., Chénier D., Bériault R., Mailloux R., Hamel R.D., Appanna V.D. Blue native polyacrylamide gel electrophoresis and the monitoring of malate- and oxaloacetate-producing enzymes. J. Biochem. Biophys. Methods 2005, 64:189-199.
27. Sonenshein A.L. Control of nitrogen metabolism by Bacillus subtilis glutamine synthetase. Mol. Microbiol. 2008, 68:242-245.
28. Stitt M., Müller C., Matt P., Gibon Y., Carillo P., Morcuende R., et al. Steps towards an integrated view of nitrogen metabolism. J. Exp. Bot. 2002, 53:959-970.
29. Tarao K., Rino Y., Ohkawa S., Shimizu A., Tamai S., Miyakawa K., et al. Association between high serum alanine aminotransferase levels and more rapid development and higher rate of incidence of hepatocellular carcinoma in patients with hepatitis C virus-associated cirrhosis. Cancer 1999, 86:589-595.
30. Taylor R.T., Jenkins W.T. Leucine aminotransferase. I. Colorimetric assay. J. Biol. Chem. 1966, 241:4391-4395.
31. Vozarova B., Stefan N., Lindsay R.S., Saremi A., Pratley R.E., Bogardus C., et al. High alanine aminotransferase is associated with decreased hepatic insulin sensitivity and predicts the development of type 2 diabetes. Diabetes 2002, 51:1889-1895.
32. Wang J.-B., Erickson J.W., Fuji R., Ramachandran S., Gao P., Dinavahi R., et al. Targeting mitochondrial glutaminase activity inhibits oncogenic transformation. Cancer Cell 2010, 18:207-219.
33. Wu K., Aoki C., Elste A., Rogalski-Wilk A.A., Siekevitz P. The synthesis of ATP by glycolytic enzymes in the postsynaptic density and the effect of endogenously generated nitric oxide. Proc. Natl. Acad. Sci. U. S. A. 1997, 94:13273-13278.
34. Yamaguchi H., Ohtani M., Amachi S., Shinoyama H., Fujii T. Some properties of glycine aminotransferase purified from Rhodopseudomonas palustris no. 7 concerning extracellular porphyrin production. Biosci. Biotechnol. Biochem. 2003, 67:783-789.