Efficient stable isotope labeling and purification of vitamin D receptor from inclusion bodies

Protein Expression and Purification

Volumn 85, Issue 1, 2012, Pages 25-31

  Zhu, Jinge ^a   Rao, Hongyu ^a   Tonelli, Marco ^a   Westler, William M ^a   Singarapu, Kiran K ^a   Markley, John L ^a   Deluca, Hector F ^a   Assadi Porter, Fariba M ^a  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

Author keywords

Escherichia coli expression; Inclusion bodies; Isotope labeling; NMR (nuclear magnetic resonance); Vitamin D receptor

Indexed keywords

CALCITRIOL RECEPTOR; DEUTERIUM; DNA; LIGAND; RECOMBINANT PROTEIN;

ANIMAL; ARTICLE; BINDING SITE; CELL INCLUSION; CHEMISTRY; ESCHERICHIA COLI; GENETICS; ISOLATION AND PURIFICATION; ISOTOPE LABELING; METABOLISM; METHODOLOGY; MOLECULAR CLONING; NUCLEAR MAGNETIC RESONANCE; PROTEIN FOLDING; PROTEIN TERTIARY STRUCTURE; RAT; SOLUBILITY;

ANIMALS; BINDING SITES; CLONING, MOLECULAR; DEUTERIUM; DNA; ESCHERICHIA COLI; INCLUSION BODIES; ISOTOPE LABELING; LIGANDS; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; PROTEIN FOLDING; PROTEIN STRUCTURE, TERTIARY; RATS; RECEPTORS, CALCITRIOL; RECOMBINANT PROTEINS; SOLUBILITY;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; EUKARYOTA; PROKARYOTA; RATTUS;

EID: 84864063359     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2012.06.012     Document Type: Article

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