Screening of environmental DNA libraries for the presence of genes conferring Na+(Li+)/H+ antiporter activity on Escherichia coli: Characterization of the recovered genes and the corresponding gene products

Journal of Bacteriology

Volumn 183, Issue 22, 2001, Pages 6645-6653

  Majernik A ^a   Gottschalk, G ^a   Daniel, R ^a  

^a UNIVERSITY OF GÖTTINGEN   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

GENE PRODUCT; LITHIUM ION; PROTON; SODIUM ION;

ARTICLE; CONTROLLED STUDY; DNA LIBRARY; ESCHERICHIA COLI; GENE INSERTION; GENE SEQUENCE; GENETIC ANALYSIS; GENETIC RECOMBINATION; MEMBRANE VESICLE; MICHAELIS MENTEN KINETICS; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PHENOTYPE; PLASMID; PRIORITY JOURNAL; PROTON SODIUM EXCHANGE; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY;

AMINO ACID SEQUENCE; DNA TRANSPOSABLE ELEMENTS; ESCHERICHIA COLI; GENE LIBRARY; GENES, BACTERIAL; HYDROGEN-ION CONCENTRATION; LITHIUM CHLORIDE; MOLECULAR SEQUENCE DATA; MUTATION; PLASMIDS; PROTEIN STRUCTURE, SECONDARY; SEQUENCE ALIGNMENT; SODIUM CHLORIDE; SODIUM-HYDROGEN ANTIPORTER; SOIL MICROBIOLOGY;

ESCHERICHIA COLI; NEGIBACTERIA; UNIDENTIFIED AGENTS;

EID: 0034749815     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.183.22.6645-6653.2001     Document Type: Article

References (47)

1. Phylogenetic identification and in situ detection of individual microbial cells without cultivation
2. The complete genome sequence of Escherichia coli K-12
3. Chitinases from uncultured microorganisms
4. A comprehensive set of sequence analysis programs for the VAX
5. A pH-dependent conformational change of NhaA Na(+)/H(+) antiporter of Escherichia coli involves loop VIII-IX, plays a role in the pH response of the protein, and is maintained by the pure protein in dodecyl maltoside
6. + antiporter in Escherichia coli
7. + antiporter gene of Escherichia coli
8. Studies on plant amylase. I. The effect of starch concentration upon the velocity of hydrolysis by the amylase of germinated barley
9. Construction of environmental DNA libraries in Escherichia coli and screening for the presence of genes conferring utilization of 4-hydroxybutyrate
10. Screening of environmental DNA libraries for the presence of genes conferring lipolytic activity on Escherichia coli
11. + antiporter from Staphylococcus aureus
12. + antiporter
13. + antiporter (NhaA) from Escherichia coli
14. + antiporters of Helicobacter pylori in antiporter-deficient Escherichia coli mutants
15. + antiporter gene
16. + antiporter activity in Escherichia coli
17. +-dependence of alkaliphility in Bacillus
18. + antiporter of Vibrio parahaemolyticus
19. A simple method for displaying the hydropathic character of a protein
20. Structure of the DNA distal to the gene for ribosomal protein S20 in Escherichia coli K12: Presence of a strong terminator and an IS1 element
21. Suicidal genetic elements and their use in biological containment of bacteria
22. + antiporter gene from the marine bacterium Vibrio alginolyticus
23. + antiporter of Vibrio parahaemolyticus
24. + antiporter, NhaD, of Vibrio parahaemolyticus
25. + antiporter (NhaA) from Escherichia coli by random mutagenesis
26. + antiporter of Escherichia coli is exposed and faces the cell exterior
27. + antiporters
28. + antiporter system(s)
29. Genome sequence of enterohaemorrhagic Escherichia coli O157:H7
30. Cloning, sequencing and expression of the nhaA and nhaR genes from Salmonella enteritidis
31. + antiporter in Escherichia coli
32. A family of genes encoding a cell-killing function may be conserved in all gram-negative bacteria
33. + antiporter of Escherichia coli. Cysteine (H226C) or serine (H226S) retain both normal activity and pH sensitivity, aspartate (H226D) shifts the pH profile toward basic pH, and alanine (H226A) inactivates the carrier at all pH values
34. + antiporter as well as the growth phenotype of Escherichia coli
35. Characterization of cell lysis in Pseudomonas putidas induced upon expression of heterologous killing genes
36. Cloning the soil metagenome: A strategy for accessing the genetic and functional diversity of uncultured microorganisms
37. Ion extrusion systems in Escherichia coli
38. + antiporter from Escherichia coli
39. Functional expression of the Enterococcus hirae NaH-antiporter in Escherichia coli
40. + antiporter coded by nhaA (ant) from Escherichia coli
41. Gene transfer among bacteria in soil and aquatic environments: A review
42. Principles governing amino acid composition of integral membrane proteins: Application to topology prediction
43. + on cell growth and pyruvate kinase activity of Escherichia coli
44. + antiporter gene from Pseudomonas aeruginosa
45. + antiporter involved in environmental survival of Vibrio cholerae
46. Proton/sodium ion transport in Escherichia coli