Expression, purification, refolding and characterization of a putative lysophospholipase from Pyrococcus furiosus: Retention of structure and lipase/esterase activity in the presence of water-miscible organic solvents at high temperatures

Protein Expression and Purification

Volumn 59, Issue 2, 2008, Pages 327-333

  Chandrayan, Sanjeev Kumar ^a   Dhaunta, Neeraj ^a   Guptasarma, Purnananda ^a,b  

^a INSTITUTE OF MICROBIAL TECHNOLOGY   (India)

^b INDIAN INSTITUTE OF SCIENCE EDUCATION AND RESEARCH MOHALI   (India)

Author keywords

Esterases; Heat active enzymes; Lipases; Solvent stable enzymes; Thermostable enzymes

Indexed keywords

BACTERIAL PROTEIN; LYSOPHOSPHOLIPASE; SOLVENT; WATER;

AMINO ACID SEQUENCE; ARTICLE; BIOSYNTHESIS; CHEMISTRY; DIMERIZATION; ENZYME STABILITY; ENZYMOLOGY; ESCHERICHIA COLI; GENETICS; HEAT; ISOLATION AND PURIFICATION; MOLECULAR GENETICS; MOLECULAR WEIGHT; PH; PROTEIN FOLDING; PROTEIN SECONDARY STRUCTURE; PROTEIN TERTIARY STRUCTURE; PYROCOCCUS FURIOSUS;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; DIMERIZATION; ENZYME STABILITY; ESCHERICHIA COLI; HEAT; HYDROGEN-ION CONCENTRATION; LYSOPHOSPHOLIPASE; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; PROTEIN FOLDING; PROTEIN STRUCTURE, SECONDARY; PROTEIN STRUCTURE, TERTIARY; PYROCOCCUS FURIOSUS; SOLVENTS; WATER;

ESCHERICHIA COLI; PYROCOCCUS FURIOSUS;

EID: 42749091887     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2008.02.019     Document Type: Article

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