Effect of Embryonic Genotype on Reference Gene Selection for RT-qPCR Normalization

Reproduction in Domestic Animals

Volumn 47, Issue 4, 2012, Pages 629-634

  Llobat, L ^a   Marco Jimenez F ^a   Penaranda D S ^a   Saenz de Juano, M D ^a   Vicente, J S ^a  

^a UNIVERSITAT POLITÈCNICA DE VALÈNCIA   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

EPIDERMAL GROWTH FACTOR RECEPTOR 3; GAMMA INTERFERON; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; HISTONE; MESSENGER RNA; OCTAMER TRANSCRIPTION FACTOR 4; TRANSFORMING GROWTH FACTOR BETA2; VASCULOTROPIN A;

ANIMAL; ANIMAL DISEASE; ANIMAL EMBRYO; ARTICLE; BLASTOCYST; CHEMISTRY; GENE EXPRESSION; GENE EXPRESSION PROFILING; GENETICS; GENOTYPE; METABOLISM; PRENATAL DEVELOPMENT; RABBIT; REAL TIME POLYMERASE CHAIN REACTION;

ANIMALS; BLASTOCYST; EMBRYO, MAMMALIAN; GENE EXPRESSION; GENE EXPRESSION PROFILING; GENOTYPE; GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASES; HISTONES; INTERFERON-GAMMA; OCTAMER TRANSCRIPTION FACTOR-3; RABBITS; REAL-TIME POLYMERASE CHAIN REACTION; RECEPTOR, ERBB-3; RNA, MESSENGER; TRANSFORMING GROWTH FACTOR BETA2; VASCULAR ENDOTHELIAL GROWTH FACTOR A;

ORYCTOLAGUS CUNICULUS;

EID: 84863785996     PISSN: 09366768     EISSN: 14390531     Source Type: Journal    
DOI: 10.1111/j.1439-0531.2011.01934.x     Document Type: Article

References (32)

1. Andersen CL, Jensen JL, Orntoft TF, 2004: Normalization of real-time quantitative reverse transcription-PCR data: a model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets. Cancer Res 64, 5245-5250.
2. Bettegowda A, Patel O, Ireland JJ, Smith GW, 2006: Quantitative analysis of messenger RNA abundance for ribosomal protein L-15, cyclophilin-A, phosphoglycerokinase, β-glucuronidase, glyceraldehydes 3-phosphate dehydrogenase. beta-actin, and histone H2A during bovine oocyte maturation and early embryogenesis in vitro. Mol Reprod Dev 73, 267-278.
3. Bower NI, Moser RJ, Hill JR, Lehnert SA, 2007: Universal reference method for real-time PCR gene expression analysis of preimplantation embryos. BioTechniques, 42, 199-206.
4. Dheda K, Huggett JF, Chang JS, Kim LU, Bustin SA, Johnson MA, Rook GAW, Zumla A, 2005: The implications of using an inappropriate reference gene for real-time reverse transcription PCR data normalization. Anal Biochem 344, 141-143.
5. Estany J, Camacho J, Baselga M, Blasco A, 1992: Selection response of growth rate in rabbits for meat production. Genet Sel Evol 24, 527-537.
6. Fischer S, Navarrete-Santos A, Thieme R, Ramin N, Fischer B, 2010: Adiponectin stimulates glucose uptake in rabbit blastocysts. Biol Reprod 110, 859-865.
7. Goossens K, Van Poucke M, Van Soom A, Vandesompele J, Van Zeveren A, Peelman LJ, 2005: Selection of reference genes for quantitative real-time PCR in bovine preimplantation embryos. BMC Dev Biol 5, 27.
8. Hellemans J, Mortier G, De Paepe A, Speleman F, Vandesompele J, 2007: qBase relative quantification framework and software for management and automated analysis of real-time quantitative PCR data. Genome Biol 8, R19.
9. Huggett J, Dheda K, Bustin S, Zumla A, 2005: Real-time RT-PCR normalisation; strategies and considerations. Genes Immun 6, 279-284. Review.
10. Kuijk EW, du Puy L, van Tol HT, Haagsman HP, Colenbrander B, Roelen BAJ, 2007: Validation of reference genes for quantitative real-time PCR studies in porcine oocytes and preimplantation embryos. BMC Dev Biol 7, 58.
11. Lazzari G, Colleoni S, Duchi R, Galli A, Houghton FD, Galli C, 2011: Embryonic genotype and inbreeding affect preimplantation development in cattle. Reproduction 141, 625-632.
12. Livak KJ, Schmittgen TD, 2001: Analysis of relative gene expression data using real-time quantitative PCR and the 2ΔΔCt Method. Methods 25, 402-408.
13. Llobat L, Marco-Jiménez F, Peñaranda DS, Thieme R, Navarrete A, Vicente JS, 2011: mRNA expression in rabbit blastocyst and endometrial tissue of candidate gene involved in gestational losses. Reprod Dom Anim, doi:.
14. Makarevich AV, Olexikoval L, Chrenek P, Kubovicova E, Freharova K, Pivko J, 2007: The effect of hyperthermia in vitro on stability of rabbit preimplantation embryos. Physiol Res 56, 789-796.
15. Mamo S, Gal AB, Bodo S, Dinnyes A, 2007: Quantitative evaluation and selection of reference genes in mouse oocytes and embryos cultured in vivo and in vitro. BMC Dev Biol 7, 14.
16. Mamo S, Gal AB, Polgar Z, Dinnyes A, 2008: Expression profiles of the pluripotency marker gene POU5f1 and validation of reference genes in rabbit oocytes and preimplantation stage embryos. BMC Mol Biol 9, 67.
17. Nailis H, Coenye T, Nieuwerburgh FV, Deforce D, Nelis HJ, 2006: Development and evaluation of different normalization strategies for gene expression studies in Candida albicans biofilms by real-time PCR. BMC Mol Biol 7, 25.
18. Navarrete-Santos A, Tonack S, Kirstein M, Pantaleon M, Kaye P, Fischer B, 2004: Insulin acts via mitogen-activated protein kinase phosphorylation in rabbit blastocysts. Reproduction 128, 517-526.
19. Navarrete-Santos A, Ramin N, Tonack S, Fischer B, 2008: Cell lineage-specific signaling of insulin and insulin-like growth factor I in rabbit blastocysts. Endocrinology 149, 515-524.
20. Pfaffl MW, 2001: A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res 29, e45.
21. Pfaffl MW, Tichopad A, Prgomet C, Neuvians TP, 2004: Determination of stable housekeeping genes, differentially regulated target genes, and sample integrity: BestKeeper-Excel-based tool using pair-wise correlations. Biotechnol Lett 26, 509-515.
22. Pierzynski P, Gajda B, Smorag Z, Rasmussen AD, Kuczynski W, 2007: Effect of atosiban on rabbit embryo development and human sperm motility. Fertil Steril 87, 1147-1152.
23. Ramin N, Thieme R, Fischer S, Schindler M, Schmidt T, Fischer B, Navarrete-Santos A, 2010: Maternal diabetes impairs gastrulation and insulin and IGF-I receptor expression in rabbit blastocysts. Endocrinology 151, 4158-4167.
24. Robert C, McGraw S, Massicotte L, Pravetoni M, Gandolfi F, Sirard MA, 2002: Quantification of housekeeping transcript levels during the development of bovine preimplantation embryos. Biol Reprod 67, 1465-1472.
25. Saenz-de-Juano MD, Peñaranda DS, Marco-Jiménez F, Llobat L, Vicente JS, 2011: Differential mRNA expression in rabbit in vivo pre-implantatory embryos. Reprod Domest Anim 46, 567-572.
26. Scharlaken B, de Graaf DC, Goossens K, Brunain M, Peelman LJ, Jacobs FJ, 2008: Reference gene selection for insect expression studies using quantitative real-time PCR: the head of the honeybee, Apis mellifera, after a bacterial challenge. J Insect Sci 33, 1-10.
27. Schmittgen TD, Zakrajsek BA, 2000: Effect of experimental treatment on housekeeping gene expression: validation by real-time, quantitative RT-PCR. J Biochem Biophys Methods 46, 69-81.
28. Thellin O, Zorzi W, Lakaye B, De Borman B, Coumans B, Hennen G, Grisar T, Igout A, Heinen E, 1999: Housekeeping genes as internal standards: use and limits. J Biotechnol 75, 291-295.
29. Tricarico C, Pinzani P, Bianchi S, Paglierani M, Distante V, Pazzagli M, Bustin SA, Orlando C, 2002: Quantitative real-time reverse transcription polymerase chain reaction: normalization to rRNA or single housekeeping genes is inappropriate for human tissue biopsies. Anal Biochem 309, 293-300.
30. Vandesompele J, De Preter K, Pattyn F, Poppe B, Van Roy N, De Paepe A, Speleman F, 2002: Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol 3, 0034.1-0034.11.
31. Vicente JS, Llobat L, Viudes-de-Castro MP, Lavara R, Baselga M, Marco-Jiménez F, 2011: Gestational losses in a rabbit line selected for growth rate. Theriogenology, doi:.
32. Willems E, Mateizel I, Kemp C, Cauffman G, Sermon K, Leyns L, 2006: Selection of reference genes in mouse embryos and in differentiating human and mouse ES cells. Int J Dev Biol 50, 627-635.