Identification and validation of reference genes for expression studies in human keratinocyte cell lines treated with and without interferon-γ - a method for qRT-PCR reference gene determination

Experimental Dermatology

Volumn 21, Issue 8, 2012, Pages 625-629

  Riemer, Angelika B ^a,b,c   Keskin, Derin B ^b   Reinherz, Ellis L ^a,c  

^a DANA FARBER CANCER INSTITUTE   (United States)

^b HARVARD MEDICAL SCHOOL   (United States)

^c GERMAN CANCER RESEARCH CENTER DKFZ   (Germany)

Author keywords

Endogenous control; Immunology; Inflammatory milieu; Interferon gamma (IFN ); Keratinocyte; Normalization; Real time polymerase chain reaction (RT PCR)

Indexed keywords

GAMMA INTERFERON; MESSENGER RNA;

ARTICLE; CELL LINE; GENE EXPRESSION; GENE FUNCTION; GENE TARGETING; GENETIC ANALYSIS; GENETIC IDENTIFICATION; HOUSEKEEPING GENE; KERATINOCYTE; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

ALGORITHMS; BETA KARYOPHERINS; CELL LINE; CYCLOPHILIN A; DNA, COMPLEMENTARY; GENE EXPRESSION; GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASES; HUMANS; INTERFERON-GAMMA; KERATINOCYTES; PHOSPHOGLYCERATE KINASE; REAL-TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; RNA, MESSENGER;

EID: 84863747104     PISSN: 09066705     EISSN: 16000625     Source Type: Journal    
DOI: 10.1111/j.1600-0625.2012.01537.x     Document Type: Article

References (32)

1. Huggett J, Dheda K, Bustin S, Zumla A. Genes Immun 2005: 6: 279-284.
2. Allen D, Winters E, Kenna P F, Humphries P, Farrar G J. J Dermatol Sci 2008: 49: 217-225.
3. de Kok J B, Roelofs R W, Giesendorf B A et al. Lab Invest 2005: 85: 154-159.
4. Bär M, Bär D, Lehmann B. J Invest Dermatol 2009: 129: 535-537.
5. Chechi K, Gelinas Y, Mathieu P, Deshaies Y, Richard D. PLoS ONE 2012: 7: e32265.
6. Mohelnikova-Duchonova B, Oliverius M, Honsova E, Soucek P. Dis Markers 2012: 32: 203-210.
7. Veazey K J, Golding M C. PLoS ONE 2011: 6: e27592.
8. Cabiati M, Raucci S, Caselli C et al. J Mol Endocrinol 2012: 48: 251-260.
9. Cinar M U, Islam M A, Uddin M J et al. BMC Res Notes 2012: 5: 107.
10. Zhang Y, Chen D, Smith M A, Zhang B, Pan X. PLoS ONE 2012: 7: e31849.
11. de Oliveira L A, Breton M C, Bastolla F M et al. Plant Cell Physiol 2012: 53: 405-422.
12. Mafra V, Kubo K S, Alves-Ferreira M et al. PLoS ONE 2012: 7: e31263.
13. Pinto F, Pacheco C C, Ferreira D, Moradas-Ferreira P, Tamagnini P. PLoS ONE 2012: 7: e34983.
14. Derveaux S, Vandesompele J, Hellemans J. Methods 2010: 50: 227-230.
15. Steenbergen R D, Hermsen M A, Walboomers J M et al. Cancer Res 1995: 55: 5465-5471.
16. Lee J H, Yi S M, Anderson M E et al. Proc Natl Acad Sci U S A 2004: 101: 2094-2099.
17. Ferris R L, Xi L, Raja S et al. Cancer Res 2005: 65: 2147-2156.
18. Vandesompele J, De Preter K, Pattyn F et al. Genome Biol 2002: 3: RESEARCH0034.1-0034. 11.
19. Andersen C L, Jensen J L, Orntoft T F. Cancer Res 2004: 64: 5245-5250.
20. Guenin S, Mauriat M, Pelloux J, Van Wuytswinkel O, Bellini C, Gutierrez L. J Exp Bot 2009: 60: 487-493.
21. Khan-Malek R, Wang Y. Methods Mol Biol 2011: 691: 227-241.
22. Bustin S A, Benes V, Nolan T, Pfaffl M W. J Mol Endocrinol 2005: 34: 597-601.
23. Campos M S, Rodini C O, Pinto-Junior D S, Nunes F D. Oral Oncol 2009: 45: 121-126.
24. Pfaffl M W, Tichopad A, Prgomet C, Neuvians T P. Biotechnol Lett 2004: 26: 509-515.
25. Wagner M, Hintner H, Bauer J W, Onder K. Exp Dermatol 2012: 21: 111-117.
26. Zhong H, Simons J W. Biochem Biophys Res Commun 1999: 259: 523-526.
27. Turabelidze A, Guo S, DiPietro L A. Wound Repair Regen 2010: 18: 460-466.
28. Balogh A, Paragh G Jr, Juhasz A et al. J Photochem Photobiol, B 2008: 93: 133-139.
29. Minner F, Poumay Y. J Invest Dermatol 2009: 129: 770-773.
30. Paragh G, Ugocsai P, Vogt T et al. Exp Dermatol 2010: 19: 297-301.
31. Brugè F, Venditti E, Tiano L, Littarru G P, Damiani E. J Biotechnol 2011: 156: 153-162.
32. Vermeulen J, De Preter K, Lefever S et al. Nucleic Acids Res 2011: 39: e63.