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Volumn 691, Issue , 2011, Pages 227-241

Statistical Analysis of Quantitative RT-PCR Results

Author keywords

Relative quantification; RT PCR; Statistical analysis

Indexed keywords

CALCULATION; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SAMPLE SIZE; ARTICLE; COMPUTER PROGRAM; METHODOLOGY; STATISTICAL ANALYSIS;

EID: 79952198814     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-60761-849-2_13     Document Type: Chapter
Times cited : (10)

References (25)
  • 1
    • 0037134017 scopus 로고    scopus 로고
    • Tech.Sight. A technique whose time has come
    • Walker, N.J. (2002) Tech.Sight. A technique whose time has come. Science 296, 557–579.
    • (2002) Science , vol.296 , pp. 557-579
    • Walker, N.J.1
  • 3
    • 24944534978 scopus 로고    scopus 로고
    • Real-time PCR for mRNA quantitation
    • Wong, M.L. and Medrano, J.F. (2005) Real-time PCR for mRNA quantitation. Biotechniques 39, 75–85.
    • (2005) Biotechniques , vol.39 , pp. 75-85
    • Wong, M.L.1    Medrano, J.F.2
  • 4
    • 8744296509 scopus 로고    scopus 로고
    • Cary, NC: SAS Institute Inc
    • SAS Institute Inc. (2004) SAS/STAT 9.1 User’s Guide. Cary, NC: SAS Institute Inc.
    • (2004) SAS/STAT 9.1 User’s Guide
  • 6
    • 21344471138 scopus 로고    scopus 로고
    • Real-time RT-PCR normalisation; strategies and considerations
    • Huggett, J., Dheda, K., Bustin, S., and Zumla, A. (2005) Real-time RT-PCR normalisation; strategies and considerations. Genes Immun. 6(4), 279–284.
    • (2005) Genes Immun , vol.6 , Issue.4 , pp. 279-284
    • Huggett, J.1    Dheda, K.2    Bustin, S.3    Zumla, A.4
  • 7
    • 0037129827 scopus 로고    scopus 로고
    • Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes
    • Vandesompele, J., De Preter, K., Pattyn, F., Poppe, B., Van Roy, N., De Paepe, A., and Speleman, F. (2002) Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol. 3(7), RESEARCH0034.
    • (2002) Genome Biol , vol.3 , Issue.7 , pp. RESEARCH0034
    • Vandesompele, J.1    De Preter, K.2    Pattyn, F.3    Poppe, B.4    Van Roy, N.5    De Paepe, A.6    Speleman, F.7
  • 8
    • 1842483281 scopus 로고    scopus 로고
    • Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: bestKeeper – Excel-based tool using pairwise correlations
    • Pfaffl, M.W., Tichopad, A., Prgomet, C., and Neuvians, T.P. (2004) Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: bestKeeper – Excel-based tool using pairwise correlations. Biotechnol. Lett. 26, 509–515.
    • (2004) Biotechnol. Lett , vol.26 , pp. 509-515
    • Pfaffl, M.W.1    Tichopad, A.2    Prgomet, C.3    Neuvians, T.P.4
  • 9
    • 18444417714 scopus 로고    scopus 로고
    • Validation of oligonucleotide microarray data using microfluidic low-density arrays: a new statistical method to normalize real-time RT-PCR data
    • Abruzzo, L.V., Lee, K.Y., Fuller, A., Silverman, A., Keating, M.J., Medeiros, L.J., and Coombes, K.R. (2005) Validation of oligonucleotide microarray data using microfluidic low-density arrays: a new statistical method to normalize real-time RT-PCR data. BioTechniques 38, 785–792.
    • (2005) BioTechniques , vol.38 , pp. 785-792
    • Abruzzo, L.V.1    Lee, K.Y.2    Fuller, A.3    Silverman, A.4    Keating, M.J.5    Medeiros, L.J.6    Coombes, K.R.7
  • 10
    • 3442876969 scopus 로고    scopus 로고
    • Normalization of real-time quantitative reverse transcription-PCR data: a model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets
    • Andersen, C.L., Jensen, J.L., and Orntoft, T.F. (2004) Normalization of real-time quantitative reverse transcription-PCR data: a model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets. Cancer Res. 64, 5245–5250.
    • (2004) Cancer Res , vol.64 , pp. 5245-5250
    • Andersen, C.L.1    Jensen, J.L.2    Orntoft, T.F.3
  • 13
    • 17344392308 scopus 로고    scopus 로고
    • A new mathematical model for relative quantification in real-time RT-PCR
    • Pfaffl, M.W. (2001) A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res. 29(9), e45.
    • (2001) Nucleic Acids Res , vol.29 , Issue.9 , pp. e45
    • Pfaffl, M.W.1
  • 14
    • 0036581160 scopus 로고    scopus 로고
    • Relative expression software tool (REST©) for group-wise comparison and statistical analysis of relative expression results in real-time PCR
    • Pfaffl, M.W., Horgan, G.W., and Dempfle, L. (2002) Relative expression software tool (REST©) for group-wise comparison and statistical analysis of relative expression results in real-time PCR. Nucleic Acids Res. 30, e36.
    • (2002) Nucleic Acids Res , vol.30 , pp. e36
    • Pfaffl, M.W.1    Horgan, G.W.2    Dempfle, L.3
  • 15
    • 31344448787 scopus 로고    scopus 로고
    • Statistical models in assessing fold change of gene expression in real-time RT-PCR experiments
    • Fu, W.J., Hu, J., Spencer, T., Carroll, R., and Wu, G. (2006) Statistical models in assessing fold change of gene expression in real-time RT-PCR experiments. Comput. Biol. Chem. 30, 21–26.
    • (2006) Comput. Biol. Chem , vol.30 , pp. 21-26
    • Fu, W.J.1    Hu, J.2    Spencer, T.3    Carroll, R.4    Wu, G.5
  • 18
    • 0242317675 scopus 로고    scopus 로고
    • Experimental validation of novel and conventional approaches to quantitative real-time PCR data analysis
    • Peirson, S.N., Butler, J.N., and Foster, R.G. (2003) Experimental validation of novel and conventional approaches to quantitative real-time PCR data analysis. Nucleic Acids Res. 31, e73.
    • (2003) Nucleic Acids Res , vol.31 , pp. e73
    • Peirson, S.N.1    Butler, J.N.2    Foster, R.G.3
  • 19
    • 0037435395 scopus 로고    scopus 로고
    • Assumption-free analysis of quantitative real-time polymerase chain reaction (PCR) data
    • Ramakers, C., Ruijter, J.M., Deprez, R.H., and Moorman, A.F. (2003) Assumption-free analysis of quantitative real-time polymerase chain reaction (PCR) data. Neurosci. Lett. 339(1), 62–66.
    • (2003) Neurosci. Lett , vol.339 , Issue.1 , pp. 62-66
    • Ramakers, C.1    Ruijter, J.M.2    Deprez, R.H.3    Moorman, A.F.4
  • 20
    • 1542356381 scopus 로고    scopus 로고
    • Standardized determination of real-time PCR efficiency from a single reaction set-up
    • Tichopad, A., Dilger, M., Schwarz, G., and Pfaffl, M.W. (2003) Standardized determination of real-time PCR efficiency from a single reaction set-up. Nucleic Acids Res. 31, e122.
    • (2003) Nucleic Acids Res , vol.31 , pp. e122
    • Tichopad, A.1    Dilger, M.2    Schwarz, G.3    Pfaffl, M.W.4
  • 21
    • 27644482283 scopus 로고    scopus 로고
    • Comprehensive algorithm for quantitative real-time polymerase chain reaction
    • Zhao, S. and Fernald, D. (2005) Comprehensive algorithm for quantitative real-time polymerase chain reaction. J. Comput. Biol. 12(8), 1047–1064.
    • (2005) J. Comput. Biol , vol.12 , Issue.8 , pp. 1047-1064
    • Zhao, S.1    Fernald, D.2
  • 22
    • 0036289137 scopus 로고    scopus 로고
    • Validation of a quantitative method for real time PCR kinetics
    • Liu, W. and Saint, D. (2002) Validation of a quantitative method for real time PCR kinetics. Biochem. Biophys. Res. Commun. 294(2), 347–353.
    • (2002) Biochem. Biophys. Res. Commun , vol.294 , Issue.2 , pp. 347-353
    • Liu, W.1    Saint, D.2
  • 23
    • 0036955404 scopus 로고    scopus 로고
    • Improving quantitative real-time RT-PCR reproducibility by boosting primer-linked amplification efficiency
    • Tichopad, A., Dzidic A., and Pfaffl, M.W. (2002) Improving quantitative real-time RT-PCR reproducibility by boosting primer-linked amplification efficiency. Biotechnol. Lett. 24(24), 2053–2056.
    • (2002) Biotechnol. Lett , vol.24 , Issue.24 , pp. 2053-2056
    • Tichopad, A.1    Dzidic, A.2    Pfaffl, M.W.3
  • 24
    • 16544384956 scopus 로고    scopus 로고
    • Sigmoidal curve-fit-ting redefines quantitative real-time PCR with the prospective of developing automated high-throughput applications
    • Rutledge, R.G. (2004) Sigmoidal curve-fit-ting redefines quantitative real-time PCR with the prospective of developing automated high-throughput applications. Nucleic Acids Res. 32(22), e178.
    • (2004) Nucleic Acids Res , vol.32 , Issue.22 , pp. e178
    • Rutledge, R.G.1


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.