The inductive effects of Centella asiatica on rat spermatogenic cell apoptosis in vivo

Journal of Natural Medicines

Volumn 66, Issue 2, 2012, Pages 271-278

  Heidari, Mahnaz ^a,b   Heidari Vala, Hamed ^a   Sadeghi, Mohammad Reza ^b   Akhondi, Mohammad Mehdi ^a  

^a SHAHID BEHESHTI UNIVERSITY   (Iran)

^b AVICENNA RESEARCH INSTITUTE   (Iran)

Author keywords

Apoptosis; Centella asiatica; Rat; Spermatogenic; Testosterone

Indexed keywords

CENTELLA ASIATICA EXTRACT; FOLLITROPIN; LUTEINIZING HORMONE; TESTOSTERONE; WATER; PLANT EXTRACT;

ACUTE TOXICITY; ADULT ANIMAL; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; APOPTOSIS; ARTICLE; BODY WEIGHT; CELL VIABILITY; CONTROLLED STUDY; DRUG ISOLATION; FOLLITROPIN BLOOD LEVEL; GERM CELL; HISTOPATHOLOGY; IN VIVO STUDY; LUTEINIZING HORMONE BLOOD LEVEL; MALE; NONHUMAN; PLANT LEAF; RAT; REPRODUCTIVE TOXICITY; SEMINIFEROUS TUBULE; SPERMATOCYTE; SPERMATOZOON COUNT; SPERMATOZOON MOTILITY; TESTIS WEIGHT; TESTOSTERONE BLOOD LEVEL; TOXICITY TESTING; ANIMAL; BLOOD; CENTELLA; CHEMISTRY; CYTOLOGY; DRUG EFFECT; PHYSIOLOGY; SPERMATOGENESIS; SPERMATOZOON; WISTAR RAT;

ANIMALS; APOPTOSIS; CENTELLA; FOLLICLE STIMULATING HORMONE; LUTEINIZING HORMONE; MALE; PLANT EXTRACTS; RATS; RATS, WISTAR; SEMINIFEROUS TUBULES; SPERM COUNT; SPERMATOGENESIS; SPERMATOZOA; TESTOSTERONE;

EID: 84863513168     PISSN: 13403443     EISSN: 18610293     Source Type: Journal    
DOI: 10.1007/s11418-011-0578-y     Document Type: Article

References (57)

1. Carlsen E, Giwercman A, Keiding N, Skakkebaek NE (1992) Evidence for decreasing quality of semen during past 50 years. Br Med J 305:609-613
2. Amann RP, Berndtson WE (1986) Assessment of procedures for screening agents for effects on male reproduction: effects of dibromochloropropane (DBCP) on the rat. Fundam Appl Toxicol 7:244-255
3. D'Cruz SC, Vaithinathan S, Jubendradass R, Mathur PP (2010) Effects of plants and plant products on the testis. Asian J Androl 12(4):468-479
4. Zheng CJ, Qin LP (2007) Chemical components of Centella asiatica and their bioactivities. Chin Integr Med 5(3):348-351
5. Kartnig T (1988) Clinical applications of Centella asiatica (L.). Herbs Spices Med Plants 3:145-173
6. Nalini K, Aroor AR, Karanth KS (1992) Effect of Centella asiatica fresh leaf aqueous extract on learning and memory and biogenic amine turnover in albino rats. Fitoterapia 63(3):232-237
7. Veerendra Kumar MH, Gupta YK (2002) Effect of different extracts of Centella asiatica on cognition and markers of oxidative stress in rats. J Ethnopharmacol 79:253-260
8. Vaidya ADB, Devasagayam TPA (2007) Current status of herbal drugs in India: an overview. J Clin Biochem Nutr 41:1-11
9. Brinkhaus B, Lindner M, Schuppan D, Hahn EG (2000) Chemical, pharmacological and clinical profile of the East Asian medical plant Centella asiatica. Phytomedicine 7(5):427-448
10. Bonfill M, Mangas S, Cusido RM, Osuna L, Pinol MT, Palazon J (2006) Indentification of friterpenoid compounds of Centella asiatica by thin layer chromatography and mass spectrometry. Biomed Chromatogr A 742:127-130
11. Inamdar PK, Yeole RD, Ghogare AB, De Souza NJ (1996) Determination of biologically active constituents in Centella asiatica. J Chromatogr A 742:127-130
12. Matsuda H, Morikawa T, Ueda H, Yoshikawa M (2001) Medicinal foodstuffs. XXVI. Saponin constituents of gotu kola (2): structures of new ursane- and oleanane-type triterpene oligoglycosides, centellasaponins B, C, and D, from Centella asiatica cultivated in Sri Lanka. Chem Pharm Bull 4:1368-1371
13. Coldren CD, Hashim P, Ali JM, Oh SK, Sinskey AJ, Rha G (2003) Gene expressing changes in the human fibroblast induced by Centella asiatica triterpenoids. Planta Med 69:725-732
14. Babu TD, Kutten G, Padikkala J (1995) Cytotoxic and antitumour properties of certain taxa of Umbelliferae with special reference to Centella asiatica (L.) Urban. J Ethnopharmacol 48:53-57
15. Lue YH, Sinha Hikim AP, Swerdloff RS, Im P, Khay ST, Bui T, Leung A, Wang C (1999) Single exposure to heat induces stagespecific germ cell apoptosis in rats: role of intratesticular testosterone on stage specificity. Endocrinology 140:1709-1717
16. Sampson JH, Raman A, Karlsen G, Navsaria H, Leigh IM (2001) In vitro keratinocyte antiproliferant effect of Centella asiatica extract and triterpenoid saponins. Phytomedicine 8:230-235
17. Shetty BS, Udupa SL, Udupa AL, Somayaji SN (2006) Effect of Centella asiatica L (Umbelliferae) on normal and dexamethasone- suppressed wound healing in Wistar Albino rats. Int J Low Extrem Wounds 5(3):137-143
18. Mutayabarwa CK, Sayi JG, Dande M (2003) Hypoglycaemic activity of Centella asiatica (L) Urb. East Cent Afr J Pharm Sci 6:30-35
19. Antony B, Santhakumari G, Merina B, Sheeba V, Mukkadan J (2006) Hepatoprotective effect of Centella asiatica (L) in carbon tetrachloride-induced liver injury in rats. Indian J Pharm Sci 68:772-776
20. Cheng CL, Guo JS, Luk J, Koo MW (2004) The healing effects of Centella extract and asiaticoside on acetic acid induced gastric ulcers in rats. Life Sci 74:2237-2249
21. Ullah MO, Sultana S, Haque A, Tasmin S (2009) Antimicrobial, cytotoxic and antioxidant activity of Centella asiatica. Eur J Sci Res 30:260-264
22. Somchit MN, Sulaiman MR, Zuraini A, Samsuddin L, Somchit N, Israf DA, Moin S (2004) Antinociceptive and antiinflammatory effects of Centella asiatica. Indian J Pharmacol 36:377-380
23. Hussin M, Abdul-Hamid A, Mohamad S, Saari N, Ismail M, Bejo MH (2007) Protective effect of Centella asiatica extract and powder on oxidative stress in rats. Food Chem 100:535-541
24. Wijeweera P, Arnason JT, Koszycki D, Merali Z (2006) Evaluation of anxiolytic properties of Gotukola-(Centella asiatica) extracts and asiaticoside in rat behavioral models. Phytomedicine 13:668-676
25. Mruk DD, Cheng CY (2004) Sertoli-sertoli and sertoli-germ cell interactions and their significance in germ cell movement in the seminiferous epithelium during spermatogenesis. Endocr Rev 25(5):747-806
26. Roosen-Runge EC (1962) The process of spermatogenesis in mammals. Biol Rev Camb Philos Soc 37:343-377
27. Canadian council on Animal Care guidelines on the care and use of form animals in research, teaching and testing (2009) CCAC, Ottawa, pp 12-15
28. Miller LC, Tainter ML (1944) Estimation of ED50 and its error by means of log probit graph. Proc Soc Exp Biol Med 57:261-264
29. Erhardt W, Hebestedt A, Aschenbrenner G, Pichotka B, Blümel G (1984) A comparative study with various anesthetics in mice (pentobarbitone, ketamine-xylazine, carfentanyl-etomidate). Res Exp Med 184:3
30. Mishra SH, Padashetty SA (2007) Effect of terpenoidal fraction of Echinops echinatus roots on reproductive parameters of male rats. J Nat Med 61(4):452-457
31. Raji Y, Udoh US, Mewoyeka OO, Onoye FC, Bolarinwa AF (2003) Implication of reproductive endocrine malfunction in male antifertility efficacy of Azadirachta indica extract in rats. J Med Med Sci 32:159-165
32. World Health Organization (1999) Laboratory manual for the examination of human semen and sperm cervical mucus interation, 4th edn. Cambridge University Press, Cambridge, pp 3-104
33. Huang HF, Pogach LM, Nathan E, Giglio W, Seebode JJ (1991) Synergistic effects of follicle-stimulating hormone and testosterone on the maintenance of spermiogenesis in hypophysectomized rats: relationship with the androgen-binding protein status. Endocrinology 28(6):3152-3161
34. Russell LD, Ettlin RA, Sinha Hikim AP, Clegg ED (1990) Histological and histopathological evaluation of the testis. Cache River Press, Clearwater
35. Sinha Hikim AP, Rajavashisth TB, Sinha Hikim I, Lue YH, Bonavera JJ, Leung A, Wang C, Swerdloff RS (1997) Significance of apoptosis in the temporal and stage-specific loss of germ cells in the adult rat after gonadotropin deprivation. Biol Reprod 57:1193-1201
36. Sinha Hikim AP, Lue YH, Swerdloff RS (1997) Separation of germ cell apoptosis from toxin induced cell death by necrosis using in situ end-labeling histochemistry after glutaraldehyde fixation. Tissue Cell 29:487-493
37. Lue YH, Sinha Hikim AP, Wang C, Bonavera JJ, Baravarian S, Leung A, Swerdloff RS (1997) Early effect of vasectomy on testicular structure and on germ cell and macrophage apoptosis. J Androl 18:166-173
38. D'Cruz SC, Vaithinathan S, Jubendradass R, Mathur PP (2010) Effects of plants and plant products on the testis. Asian J Androl 12:468-479
39. Bataineh H, Nusier M (2005) Effect of cholesterol diet on reproductive functions in male albino rats. Saudi Med J 26:398-404
40. Bataineh H, Al-Hamood MH, Elbetieha AM (1998) Assessment of aggression, sexual behavior and fertility in adult male rat following long-term ingestion of four industrial metal salts. Hum Exp Toxicol 17:570-576
41. Agrawal S, Chauhan S, Mathur R (1986) Antifertility effects of embelin in male rats. Andrologia 18:125-131
42. Dym M, Raj HG, Lin YC, Chemes HE, Kotite NJ, Nayfeh SN, French FS (1979) Is FSH required for maintenance of spermatogenesis in adult rats? J Reprod Fertil 26:175-181
43. Mahanem MN, Norazalia MA (2004) Kesan In Vivo Ekstrak Daun Centella asiatica ke atas Histologi Testis dan Kualiti Sperma Mencit (In vivo effects of Centella asiatica leaf extract on the histology of testis and sperm quality in mice). Sains Malaysiana 33(2):97-103
44. Zitzmann M (2008) Effects of testosterone replacement and its pharmacogenetics on physical performance and metabolism. Asian J Androl 10(3):364-372
45. Gupta RS, Kumar P, Dixit VP, Dobhal MP (2000) Antifertility studies of the root extract of the Barleria prionitis Linn. in male albino rats with special reference to testicular cell population dynamics. J Ethnopharmacol 70(2):111-117
46. Choi SM, Lee BM (2004) An alternative mode of action of endocrine-disrupting chemicals and chemoprevention. J Toxicol Environ Health B Crit Rev 7(6):451-463
47. Huang HFS, Linsenmeyer TA, Li MT, Giglio W, Anesetti R, von Hagen J et al (1995) Acute effects of spinal cord injury on the pituitary-testicular hormone axis and Sertoli cell functions: a time course study. J Androl 16:148-157
48. Yunianto I, Das S, Mat Noor M (2010) Antispermatogenic and antifertility effect of Pegaga (Centella asiatica L) on the testis of male Sprague-Dawley rats. Clin Ter 161(3):235-239
49. Seed J, Chapin RE, Clegg ED, Dostal LA, Foote RH, Hurtt ME, Klinefelter GR, Makris SL, Perreault SD, Schrader S, Seyler D, Sprando R, Treinen KA, Veeramachaneni DN, Wise LD (1996) Methods for assessing sperm motility, morphology, and counts in the rat, rabbit, and dog: a consensus report. ILSI Risk Science Institute Expert Working Group on Sperm Evaluation. Reprod Toxicol 10(3):237-244
50. Huynh PN, Hikim AP, Wang C, Stefonovic K, Lue YH, Leung A, Atienza V, Baravarian S, Reutrakul V, Swerdloff RS (2000) Long-term effects of triptolide on spermatogenesis, epididymal sperm function, and fertility in male rats. J Androl 21(5): 689-699
51. Lemasters GK, Selevan SG (1993) Toxic exposures and reproduction: a view of epidemiology and surveillance. In: Scialli AR, Zinaman MJ (eds) Reproductive toxicology and infertility. McGraw Hill, USA, pp 307-32
52. Working PK, Chellman GJ (1993) The testis, spermatogenesis and the excurrent duct system. In: Scialli AR, Zinaman MJ (eds) Reproductive toxicology and infertility. ISBN, McGraw Hill, USA, pp 55-76
53. Parveen S, Das S, Kundra cP, Pereira BMJ (2003) A comprehensive evaluation of the reproduction toxicity of Quassia amara in male rats. Reprod Toxicol 45(1):45-50
54. Moline JM, Golden AL, Bar-Chama N, Smith E, Rauch ME, Chapin RE et al (2000) Exposure to hazardous substances and male reproductive health: a research framework. Environ Health Perspect 108:803-813
55. Richburg JH (2000) The relevance of spontaneous-and chemically- induced alterations in testicular germ cell apoptosis to toxicology. Toxicol Lett 112-113:79-86
56. Bhat GK, Sea TL, Olatinwo MO, Simorangkir D, Ford GD, Ford BD, Mann DR (2006) Influence of a leptin deficiency on testicular morphology, germ cell apoptosis, and expression levels of apoptosis related genes in the mouse. J Androl 27(2):302-310
57. Lampiao F, Agarwal A, du Plessis SS (2009) The role of insulin and leptin in male reproduction. Arch Med Sci 5(1A):S48-S54