Integrated development of an effective bioprocess for extracellular production of penicillin G acylase in Escherichia coli and its subsequent one-step purification

Journal of Biotechnology

Volumn 161, Issue 1, 2012, Pages 19-26

  Orr, Valerie ^a   Scharer, Jeno ^a   Moo Young, Murray ^a   Honeyman, C Howie ^b   Fenner, Drew ^b,d   Crossley, Lisa ^b,d   Suen, Shing Yi ^c   Chou, C Perry ^a  

^a UNIVERSITY OF WATERLOO   (Canada)

^b Natrix Separations Inc   (Canada)

^c NATIONAL CHUNG HSING UNIVERSITY   (Taiwan)

^d BioVenturesca   (Canada)

Author keywords

Anion exchange chromatography; Escherichia coli; Extracellular secretion; Outer membrane mutant; Penicillin G acylase

Indexed keywords

ANION EXCHANGE; ANION EXCHANGE CHROMATOGRAPHY; BIOPROCESSES; BUFFERING CAPACITIES; CELL LYSIS; CHROMATOGRAPHIC MEDIA; CULTURE MEDIUM; EXPRESSION LEVELS; EXTRACELLULAR; EXTRACELLULAR MEDIUM; EXTRACELLULAR PROTEINS; EXTRACELLULAR SECRETION; FLOWTHROUGH; INCLUSION BODIES; INTEGRATED DEVELOPMENT; IONIC SPECIES; LOW CONDUCTIVITY; OLIGOPEPTIDES; ONE-STEP PURIFICATION; OSMOLARITY; OUTER-MEMBRANE MUTANT; OVER-EXPRESSION; PENICILLIN G ACYLASE; PURIFICATION FACTORS; PURIFICATION PROCESS; SECRETION EFFICIENCY; SINGLE-STEP; SPECIFIC ACTIVITY;

CELL CULTURE; CELL GROWTH; COLUMN CHROMATOGRAPHY; ENZYMES; ESCHERICHIA COLI; GENETIC ENGINEERING; GROWTH KINETICS; IONS; ULTRAFILTRATION;

PURIFICATION;

BUFFER; OLIGOPEPTIDE; PENICILLIN AMIDASE;

ANION EXCHANGE CHROMATOGRAPHY; ARTICLE; BACTERIAL STRAIN; BIOPROCESS; CELL CULTURE; CELL GROWTH; CELL INCLUSION; CELL SEPARATION; CELLULAR DISTRIBUTION; CONDUCTANCE; CULTURE MEDIUM; CYTOLYSIS; ENZYME ACTIVITY; ENZYME PURIFICATION; ENZYME RELEASE; ENZYME SYNTHESIS; ESCHERICHIA COLI; GENETIC ENGINEERING; NONHUMAN; NUTRIENT; OSMOLARITY; OSMOTIC PRESSURE; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN EXPRESSION; SPECIES CULTIVATION; ULTRAFILTRATION;

BIOTECHNOLOGY; BLOTTING, WESTERN; CHROMATOGRAPHY, ION EXCHANGE; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; GENETIC ENGINEERING; INCLUSION BODIES; INDUSTRIAL MICROBIOLOGY; PENICILLIN AMIDASE; ULTRAFILTRATION;

ESCHERICHIA COLI;

EID: 84863461824     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2012.05.013     Document Type: Article

References (37)

1. Aguilar O., Albiter V., Serrano-Carreon L., Rito-Palomares M. Direct comparison between ion-exchange chromatography and aqueous two-phase processes for the partial purification of penicillin acylase produced by E. coli. Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences 2006, 835:77-83.
2. Amersham Biosciences Ion Exchange Chromatography & Chromatofocusing: Principles and Methods 2004, GE Healthcare, Buckinghamshire, UK.
3. Balasingham K., Warburton D., Dunnill P., Lilly M.D. The isolation and kinetics of penicillin amidase from Escherichia coli. Biochimica et Biophysica Acta 1972, 276:250-256.
4. Bossi A., Cretich M., Giorgio P., Righetti P. Production of d-phenylglycine from racemic (d,l)-phenylglycine via isoelectrically-trapped penicillin G acylase. Biotechnology and Bioengineering 1998, 60:454-461.
5. Boyer H.W., Roulland-Dussoix D. A complementation analysis of the restriction and modification of DNA in Escherichia coli. Journal of Molecular Biology 1969, 41:459-472.
6. Chandel A.K., Rao L.V., Narasu M.L., Singh O.V. The realm of penicillin G acylase in β-lactam antibiotics. Enzyme and Microbial Technology 2008, 42:199-207.
7. Choi J.H., Keum K.C., Lee S.Y. Production of recombinant proteins by high cell density culture of Escherichia coli. Chemical Engineering Science 2006, 61:876-885.
8. Choi J.H., Lee S.Y. Secretory and extracellular production of recombinant proteins using Escherichia coli. Applied Microbiology and Biotechnology 2004, 64:625-635.
9. Chou C.P. Engineering cell physiology to enhance recombinant protein production in Escherichia coli. Applied Microbiology and Biotechnology 2007, 76:521-532.
10. Chou C.P., Lin W., Kuo B., Yu C. Genetic strategies to enhance penicillin acylase production in Escherichia coli. Enzyme and Microbial Technology 2000, 27:766-773.
11. Chou C.P., Yu C., Tseng J., Lin M., Lin W. Genetic manipulation to identify limiting steps and develop strategies for high-level expression of penicillin acylase in Escherichia coli. Biotechnology and Bioengineering 1999, 63:263-272.
12. Fonseca L.P., Cabral J.M.S. An integrated downstream processing strategy for the recovery and partial purification of penicillin acylase from crude media. Journal of Chemical Technology and Biotechnology 2002, 77:1176-1185.
13. Forciniti D. Industrial Bioseparations: Principles and Practice 2008, Blackwell Publishing, Ames, Iowa.
14. Fu X. Extracellular accumulation of recombinant protein by Escherichia coli in a defined medium. Applied and Environment Microbiology 2010, 88:75-86.
15. Ghosh R. Principles of Bioseparations Engineering 2006, World Scientific, Hachensack, Singapore.
16. Harcum S., Bentley E. Response dynamics of 26-, 34-, 39-, 54-, and 80kDa proteases in induced culture of recombinant Escherichia coli. Biotechnology and Bioengineering 1993, 42:675-685.
17. Hirota Y., Suzuki H., Nishimura Y., Yasuda S. On the process of cellular division in Escherichia coli: a mutant of E coli lacking a murein-lipoprotein. Proceedings of the National Academy of Sciences of the United States of America 1977, 74:1417-1420.
18. Hoffmann F., Heuvel J.v.d., Zidek N., Rinas U. Minimizing inclusion body formation during recombinant protein production in Escherichia coli at bench and pilot plant scale. Enzyme and Microbial Technology 2004, 34:235-241.
19. Hoishen C., Fritsche C., Grumpet J., Westermann M., Gura K., Fahnert B. Novel bacterial membrane surface display using cell wall-less L forms of Proteus mirabilis and Escherichia coli. Applied and Environment Microbiology 2002, 68:525-531.
20. Ignatova Z., Mahsunah A., Georgieva M., Kasche V. Improvement of posttranslational bottlenecks in the production of penicillin amidase in recombinant Escherichia coli strains. Applied and Environment Microbiology 2003, 69:1237-1247.
21. Kanamori T., Mizushima S., Shimizu Y., Morishita H., Kubota H., Nii A., Ogino H., Nagase Y., Kisargi M., Nobuhara M. Expression and excretion of human pancreatic secretory trypsin inhibitor in lipoprotein-deletion mutant of Escherichia coli. Gene 1988, 66:295-300.
22. Lee J., Choi Y., Kang S., Park H., Kwon I. Production of human leukocyte interferon in Escherichia coli by control of growth rate in fed-batch fermentation. Biotechnology Letters 1989, 11:695-698.
23. Lee S.Y. High cell-density culture of Escherichia coli. Trends in Biotechnology 1996, 14:98-105.
24. Li Y., Chen C.X., von Specht B.U., Hahn H.P. Cloning and hemolysin-mediated secretory expression of a codon optimized synthetic human interleukin-6 gene in Escherichia coli. Protein Expression and Purification 2002, 25:437-447.
25. Liu Y.-C., ChangChien C.-C., Suen S.-Y. Purification of penicillin G acylase using immobilized metal affinity membranes. Journal of Chromatography B 2003, 794:67-76.
26. Mergulhao F.J., Summers D.K., Monteiro G.A. Recombinant protein secretion in Escherichia coli. Biotechnology Advances 2005, 23:177-202.
27. Narayanan N., Follonier S., Chou C.P. In vivo monitoring and alleviation of extracytoplasmic stress to recombinant protein overproduction in the periplasm of Escherichia coli. Biochemical Engineering Journal 2008, 42:13-19.
28. Neidhardt F.C., Bloch P.L., Smith D.F. Culture medium for enterobacteria. Journal of Bacteriology 1974, 119:736-747.
29. Pall's Corporation, 2011. Western Blotting for PVDF Membranes.
30. Robbins J.W., Taylor K.B. Optimization of Escherichia coli growth by the controlled addition of glucose. Biotechnology and Bioengineering 2004, 34:1289-1294.
31. Sambrook J., Fritsch E.F., Maniatis T. Molecular Cloning: A Laboratory Manual 1989, Cold Spring Harbor Laboratory Press, New York, USA.
32. Shin H.D., Chen R.R. Extracellular recombinant protein production from an Escherichia coli lpp deletion mutant. Biotechnology and Bioengineering 2008, 101:1288-1296.
33. Shin H.D., McClendon S., Vo T., Chen R.R. Escherichia coli binary culture engineered for direct fermentation of hemicellulose to a biofuel. Applied and Environment Microbiology 2010, 76:8150-8159.
34. Sonntag I., Schwarz H., Hirota Y., Henning U. Cell envelope and shape of Escherichia coli: multiple mutants missing the outer membrane lipoprotein and other major outer membrane proteins. Journal of Bacteriology 1978, 136:280-285.
35. Towbin H., Staehelin T., Gorgon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences of the United States of America 1979, 76:4350-4354.
36. Yem D.W., Wu H.C. Physiological characterization of an Escherichia coli mutant altered in the structure of murein lipoprotein. Journal of Bacteriology 1978, 133:1419-1426.
37. Yoon S.H., Kim S.K., Kim J.F. Secretory production of recombinant proteins in Escherichia coli. Recent Patents on Biotechnology 2010, 4:23-29.