Genetic manipulation to identify limiting steps and develop strategies for high-level expression of penicillin acylase in Escherichia coli

Biotechnology and Bioengineering

Volumn 63, Issue 3, 1999, Pages 263-272

  Chou, C Perry ^a   Yu, Chih Chang ^a   Tseng, Jen Hung ^a   Lin, Ming I ^a   Lin, Hua Kuo ^a  

^a FENG CHIA UNIVERSITY   (Taiwan)

Author keywords

Escherichia coli; Gene expression; Genetic manipulation; Penicillin acylase; Recombinant DNA technology

Indexed keywords

BIOTECHNOLOGY; DNA SEQUENCES; ENZYMES; GENE TRANSFER;

GENE EXPRESSION; GENETIC MANIPULATION; PENICILLIN ACYLASE;

GENETIC ENGINEERING;

PENICILLIN AMIDASE;

ARTICLE; ENZYME ASSAY; ENZYME SYNTHESIS; ESCHERICHIA COLI; GENE EXPRESSION REGULATION; GENE STRUCTURE; GENETIC MANIPULATION; NONHUMAN; PROTEIN EXPRESSION; RECOMBINANT DNA TECHNOLOGY; REGULATOR GENE; STRUCTURE ANALYSIS; TRANSCRIPTION REGULATION; TRANSLATION REGULATION;

CLONING, MOLECULAR; DNA, RIBOSOMAL; ESCHERICHIA COLI; GENE EXPRESSION REGULATION, BACTERIAL; GENE EXPRESSION REGULATION, ENZYMOLOGIC; GENES, BACTERIAL; KINETICS; PENICILLIN AMIDASE; PLASMIDS; PROTEIN BIOSYNTHESIS; REGULATORY SEQUENCES, NUCLEIC ACID; RESTRICTION MAPPING; SERRATIA MARCESCENS; TRANSCRIPTION, GENETIC;

ESCHERICHIA COLI; NEGIBACTERIA; UNIDENTIFIED BACTERIOPHAGE;

EID: 0033526409     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0290(19990505)63:3<263::AID-BIT2>3.0.CO;2-T     Document Type: Article

References (38)

1. Amann E, Ochs B, Abel KJ. 1988. Tightly regulated tac promoter vectors useful for the expression of unfused and fused proteins in Escherichia coll. Gene 69:301-315.
2. Balasingham K, Warburton D, Dunnill P, Lilly MD. 1972. The isolation and kinetics of penicillin amidase from Escherichia coli. Biochim Biophys Acta 276:250-256.
3. Bhattacharya S, Gupta VS, Prabhune AA, SivaRaman H, Debnath M, Ranjekar PK 1993. Studies of operational variables in batch mode for genetically engineered Escherichia coli cells containing penicillin acylase. Enzyme Microb Technol 15:1070-1073.
4. Bolivar F, Rodriguez RL, Greene PJ, Beltach MC, Heyneker HL, Boyer HW, Crosa JH, Falkow S. 1977. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. Gene 2: 95-113.
5. Bowden GA, Paredes AM, Georgiou G. 1991. Structure and morphology of protein inclusion bodies in Escherichia coll. Bio/Technology 9: 725-730.
6. Boyer HW, Roulland-Dussoix D. 1969. A complementation analysis of the restriction and modification of DNA in Escherichia coli. J Mol Biol 41:459-472.
7. Chang ACY, Cohen SN. 1978. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the p15A cryptic miniplasmid. J Bacteriol 134:1141-1156.
8. Chou CP, Tseng J-H, Lin M-I, Lin H-K, Yu C-C. 1999. Manipulation of carbon assimilation with respect to expression of the pac gene for improving production of penicillin acylase in Escherichia coli. J Biotechnol. to appear.
9. de Smit MH, van Duin J. 1990. Secondary structure of the ribosome binding site determines translational efficiency: A quantitative analysis. Proc Natl Acad Sci USA 87:7668-7672.
10. Gang DM, Shaikh K. 1976. Regulation of penicillin acylase in Escherichia coli by cyclic AMP. Biochim Biophys Acta 425:110-114.
11. Goeddel DV, editor. 1990. Methods in Enzymology (vol. 185): Gene expression technology. San Diego: Academic Press.
12. Gualerzi CO, Pon CL. 1990. Initiation of mRNA translation in prokaryotes. Biochemistry-USA 29:5881-5889.
13. Keilmann C, Wanner G, Bock A. 1993. Molecular basis of the exclusive low-temperature synthesis of an enzyme in E. coli: Penicillin acylase. Biol Chem. Hoppe-Seyler 374:983-992.
14. Leipold RJ, Dhurjati P. 1993. Construction and characterization of a specialized ribosome system for the overproduction of proteins in Escherichia coli. Biotechnol Prog 9:345-354.
15. Looman AC, Bodlaender J, Comstock LJ, Eaton D, Jhurani P, de Boer H A, van Knippenberg PH. 1987. Influence of the codon following the AUG initiation codon on the expression of a modified lacZ gene in Escherichia coli. EMBO J 6:2489-2492.
16. Marinus MG. 1973. Location of DNA methylation genes on the Escherichia coli K-12 genetic map. Mol Gen Genet 127:47-55.
17. Meevootisom V, Somsuk P, Prachaktam R, Flegel TW. 1983. Simple screening method for isolation of penicillin acylase-producing bacteria. Appl Environ Microbiol 46:1227-1229.
18. Merino E, Balbas P, Reculas F, Becerril B, Valle F, Bolivar F. 1992. Carbon regulation and the role in nature of the Escherichia coli penicillin acylase (pac) gene. Mol Microbiol 6:2175-2182.
19. Oh S-J, Kim Y-C. Park Y-W, Min S-Y, Kim I-S, Kang H-S. 1987. Complete nucleotide sequence of the penicillin G acylase gene and the flanking regions, and its expression in Escherichia coli. Gene 56: 87-97.
20. Petersen C. 1991. Multiple determinants of functional mRNA stability: Sequence alterations at either end of the lacZ gene affect the rate of mRNA inactivation. J Bacteriol 173:2167-2172.
21. Ramirez OT, Zamora R, Espinosa G, Merino E, Bolivar F, Quintero R, 1994a. Kinetic study of penicillin acylase production by recombinant E. coli in batch cultures. Process Biochem 29:197-206.
22. Ramirez OT, Zamora R, Quintero R, Lopez-Munguia A 1994h. Exponentially fed-batch cultures as an alternative to chemostats: The case of penicillin acylase production by recombinant E. coli. Enzyme Microb Technol 16:895-903.
23. Robas N, Zouheiry H, Branlant G, Branlant C. 1993. Improved penicillin amidase production using a genetically engineered mutant of Escherichia coli ATCC 11105. Biotechnol Bioeng 41: 14-24.
24. Sambrook J, Fritsch E F. Maniatis T. 1989. Molecular Cloning: A Laboratory Manual. New York: Cold Spring Harbor Laboratory Press.
25. Scherrer S, Robas N, Zouheiry H, Branlant G, Branlant C. 1994. Periplasmic aggregation limits the proteolytic maturation of the Escherichia coli Penicillin G amidase Precursor polypeptide. Appl Microbiol Biotechnol 42:85-91.
26. Shewale J G, Deshpande B S, Sudhakaran V K, Ambedkar S S. 1990. Penicillin acylases: Applications and potentials. Process Biochem 25: 97-103.
27. Shewale J G, Sivaraman H. 1989. Penicillin acylase: Enzyme production and its application in the manufacture of 6-APA. Process Biochem 24: 146-154.
28. Sizmann D, Keilmann C, Bock A. 1990. Primary structure requirements for the maturation in vivo of penicillin acylase from Escherichia coli ATCC 11105. Eur J Biochem 192:143-151.
29. Smith D W E. 1996. Problems of translating heterologous genes in expression systems: The role of tRNA. Biotechnol Prog 12:417-422.
30. Sobotkova L, Stepanek V, Plhackova K, Kyslik P. 1995. Cloning of penicillin G acylase-encoding gene from Escherichia coli high-producing strain RE3. Biotechnol Lett 17:723-728.
31. Sobotkova L, Stepanek V, Plhackova K, Kyslik P. 1996. Development of a high-expression system for penicillin G acylase based on the recom-binant Escherichia coli strain RE3(pKA18). Enzyme Microb Technol 19:389-397.
32. Sriubolmas N, Panbangred W, Sriurairatana S, Meevootisom V. 1997. Localization and characterization of inclusion bodies in recombinant Escherichia coli cells overproducing penicillin G acylase. Appl Microbiol Biotechnol 47:373-378.
33. Stormo G D. 1986. Translation initiation. In: Reznikoff W and Gold, L. editors. Maximizing Gene Expression. Boston: Butterworths. p 195-224.
34. Strandberg L, Enfors S-O. 1991. Factors influencing inclusion body formation in the production of a fused protein in Escherichia coli. Appl Environ Microbiol 57:1669-1674.
35. Towbin H, Staehelin T, Gordon J. 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Nat Acad Sci USA 76:4350-4354.
36. Valle F, Gosset G, Tenorio B, Oliver G, Bolivar F. 1986 Characterization of the regulatory region of the Escherichia coli penicillin acylase structural gene. Gene 50:119-122.
37. Vandamme E J 1980. Penicillin acylases and β-lactamases. In: Rose A H. editor. Economic microbiology, vol 5: Microbial enzymes and bioconversions. New York: Academic Press, p 467-522.
38. Vind J, Sorensen M A, Rasmussen M D, Pedersen S. 1993. Synthesis of proteins in Escherichia coli is limited by the concentration of free ribosomes: Expression from reporter genes does not always reflect functional mRNA levels. J Mol Biol 231:678-688.