Novel bacterial membrane surface display system using cell wall-less L-forms of Proteus mirabilis and Escherichia coli

Applied and Environmental Microbiology

Volumn 68, Issue 2, 2002, Pages 525-531

  Hoischen, Christian ^a   Fritsche, Christine ^a   Gumpert, Johannes ^a   Westermann, Martin ^a   Gura, Katleen ^a   Fahnert, Beatrix ^a  

^a FRITZ LIPMANN INSTITUTE   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CELL MEMBRANES; ELECTRON MICROSCOPY; MORPHOLOGY; PROTEINS;

MEMBRANE SURFACE;

ESCHERICHIA COLI;

CARRIER PROTEIN; ENDOTOXIN; HYBRID PROTEIN; LACTOSE PERMEASE; MEMBRANE PROTEIN; PROTEIN PRECURSOR; STAPHYLOKINASE;

MEMBRANE;

ARTICLE; BACTERIAL CELL WALL; BACTERIAL MEMBRANE; CELL FRACTIONATION; CELL MEMBRANE; CELL STRUCTURE; ELECTRON MICROSCOPY; ESCHERICHIA COLI; IMMUNOGOLD LABELING; NONHUMAN; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; PROTEIN MOTIF; PROTEIN PURIFICATION; PROTEUS MIRABILIS; STRAIN DIFFERENCE;

BACTERIAL OUTER MEMBRANE PROTEINS; CELL MEMBRANE; ESCHERICHIA COLI; L FORMS; MEMBRANE PROTEINS; METALLOENDOPEPTIDASES; MICROSCOPY, ELECTRON; PROTEUS MIRABILIS; RECOMBINANT FUSION PROTEINS; TRYPSIN;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; MIRABILIS; NEGIBACTERIA; PROTEUS MIRABILIS;

EID: 0036156641     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.68.2.525-531.2002     Document Type: Article

References (35)

1. Topology analysis of the SecY protein, an integral membrane protein involved in protein export in Escherichia coli
2. Cloning and expression in Escherichia coli, Bacillus subtilis, and Streptococcus sanguis of a gene for staphylokinase - A bacterial plasminogen activator
3. A rapid and sensitive method for the quantification of microgram quantities of proteins utilizing the principle of protein-dye binding
4. Expression of the carboxypeptidase T gene from Thermoactinomyces vulgaris in stable cell wall-less L-forms of Proteus mirabilis
5. Expressing genes in different Escherichia coli compartments
6. A new membrane-bound OprI lipoprotein expression vector. High production of heterologous fusion proteins in gram-negative bacteria and the implications for oral vaccination
7. Structure of the lac carrier protein of Escherichia coli
8. SDS-digested freeze-fracture replica labeling electron microscopy to study the two-dimensional distribution of integral membrane proteins and phospholipids in biomembranes: Practical procedure, interpretation, and application
9. Display of heterologous proteins on the surface of microorganisms: From the screening of combinatorial libraries to live recombinant vaccines
10. Display of β-lactamase on the Escherichia coli surface: Outer membrane phenotypes conferred by Lpp'- OmpA'-β-lactamase fusions
11. Use of cell wall-less bacteria (L-forms) for efficient expression and secretion of heterologous gene products
12. Characteristic properties and biological significance of stable protoplast type L-forms
13. Synthesis and secretion of recombinant penicillin G acylase in bacterial L-forms
14. A novel membrane protein influencing cell shape and multicellular swarming of Proteus mirabilis
15. Lipid and fatty acid composition of cytoplasmic membranes from Streptomyces hygroscopicus and its stable protoplast-type L-form
16. Surface display of Zymomonas mobilis levansucrase by using the ice-nucleation protein of Pseudomonas syringae
17. The interaction between aspartic acid 237 and lysine 358 in the lactose carrier of Excherichia coli
18. Cell surface presentation of recombinant (poly)peptides including functional T-cell epitopes by the AIDA autotransporter system
19. Expression and secretion of functional miniantibodies McPC603scFvDhlx in cell wall-less L-form strains of Proteus mirabilis and Escherichia coli
20. Outer membrane proteins as surface display systems
21. Characterization of adhesive epitopes with the OmpS display system
22. Surface-displayed viral antigens on Salmonella carrier vaccine
23. Anchoring of proteins to lactic acid bacteria
24. Production of recombinant subunit vaccines: Protein immunogens, live delivery systems and nucleic acid vaccination
25. Expression of thioredoxin random peptide libraries on the Escherichia coli cell surface as functional fusions to flagellin: A system designed for exploring protein-protein interactions
26. Autodisplay: One-component system for efficient surface display and release of soluble recombinant proteins from Escherichia coli
27. Separation of inner and outer membrane vesicles from Escherichia coli in self-generating percoll gradients
28. Expression and secretion of recombinant scFv antibodies in L-form cells lead to active product and overcomes the limitations of periplasmic expression in E. coli
29. NH2-terminal structural motifs in staphylokinase required for plasminogen activation
30. Applications of S-layers
31. Bacterial surface display: Trends and progress
32. Expression of membrane proteins in Escherichia coli
33. Engineering a mouse metallothionein on the cell surface of Ralstonia eutropha CH34 for immobilization of heavy metals in soil