Targeting Vector Construction Through Recombineering

Methods in Molecular Biology

Volumn 693, Issue , 2011, Pages 181-203

  Malureanu, Liviu A ^a  

^a Mayo Clinic College of Medicine   (United States)

Author keywords

Conditional knockout; Gene targeting; Mutagenesis; Recombineering

Indexed keywords

ANIMAL; ARTICLE; BACTERIAL ARTIFICIAL CHROMOSOME; BACTERIOPHAGE; GENE VECTOR; GENETIC RECOMBINATION; GENETICS; MOUSE; MOUSE MUTANT; POLYMERASE CHAIN REACTION;

ANIMALS; BACTERIOPHAGES; CHROMOSOMES, ARTIFICIAL, BACTERIAL; GENETIC VECTORS; MICE; MICE, KNOCKOUT; POLYMERASE CHAIN REACTION; RECOMBINATION, GENETIC;

MLCS; MLOWN;

EID: 79952277668     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-60761-974-1_11     Document Type: Chapter

References (16)

1. Zhang, Y., Buchholz, F., Muyrers, J. P., and Stewart, A. F. (1998) A new logic for DNA engineering using recombination in Escherichia coli, Nat Genet 20, 123–128.
2. Muyrers, J. P., Zhang, Y., Testa, G., and Stewart, A. F. (1999) Rapid modification of bacterial artificial chromosomes by ET-recombination, Nucleic Acids Res 27, 1555–1557.
3. Ellis, H. M., Yu, D., DiTizio, T., and Court, D. L. (2001) High efficiency mutagenesis, repair, and engineering of chromosomal DNA using single-stranded oligonucleotides, Proc Natl Acad Sci U S A 98, 6742–6746.
4. Copeland, N. G., Jenkins, N. A., and Court, D. L. (2001) Recombineering: a powerful new tool for mouse functional genomics, Nat Rev Genet 2, 769–779.
5. Muyrers, J. P., Zhang, Y., and Stewart, A. F. (2001) Techniques: recombinogenic engineering – new options for cloning and manipulating DNA, Trends Biochem Sci 26, 325–331.
6. Zhang, P., Li, M. Z., and Elledge, S. J. (2002) Towards genetic genome projects: genomic library screening and gene-targeting vector construction in a single step, Nat Genet 30, 31–39.
7. Lee, E. C., Yu, D., Martinez de Velasco, J., Tessarollo, L., Swing, D. A., Court, D. L., Jenkins, N. A., and Copeland, N. G. (2001) A highly efficient Escherichia coli-based chromosome engineering system adapted for recombi- nogenic targeting and subcloning of BAC DNA, Genomics 73, 56–65.
8. Warming, S., Costantino, N., Court, D. L., Jenkins, N. A., and Copeland, N. G. (2005) Simple and highly efficient BAC recombineering using galK selection, Nucleic Acids Res 33, e36.
9. http://recombineering.ncifcrf.gov/.
10. Liu, P., Jenkins, N. A., and Copeland, N. G. (2003) A highly efficient recombineering-based method for generating conditional knockout mutations, Genome Res 13, 476–484.
11. Yagi, T., Ikawa, Y., Yoshida, K., Shigetani, Y., Takeda, N., Mabuchi, I., Yamamoto, T., and Aizawa, S. (1990) Homologous recombination at c-fyn locus of mouse embryonic stem cells with use of diphtheria toxin A-fragment gene in negative selection, Proc Natl Acad Sci U S A 87, 9918–9922.
12. Soriano, P. (1997) The PDGF alpha receptor is required for neural crest cell development and for normal patterning of the somites, Development 124, 2691–2700.
13. http://www.geneser vice.co.uk/products/sanger/bMQ/bMQ.jsp.
14. Dawlaty, M. M. and van Deursen, J. M. (2006) Gene targeting methods for studying nuclear transport factors in mice, Methods 39, 370–378.
15. http://spliceport.cs.umd.edu/.
16. http://www.biology.utah.edu/jorgensen/wayned/ape/.