A novel PCR strategy for high-efficiency, automated site-directed mutagenesis

Nucleic Acids Research

Volumn 33, Issue 13, 2005, Pages 1-7

  Wu, Wu ^a   Jia, Zongchao ^b   Liu, Ping ^a   Xie, Zhigang ^a   Wei, Qun ^a  

^a BEIJING NORMAL UNIVERSITY   (China)

^b QUEEN S UNIVERSITY   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

PLASMID DNA; PRIMER DNA; SINGLE STRANDED DNA;

ARTICLE; AUTOMATION; COST EFFECTIVENESS ANALYSIS; DNA DENATURATION; DNA FLANKING REGION; DNA SEQUENCE; DNA SYNTHESIS; DNA TEMPLATE; GENE MUTATION; MELTING POINT; METHODOLOGY; MUTAGENESIS; MUTAGENIC ACTIVITY; MUTANT; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SEQUENCE ANALYSIS; SITE DIRECTED MUTAGENESIS; TEMPERATURE SENSITIVITY; WILD TYPE; EVALUATION; TEMPERATURE;

DNA PRIMERS; MUTAGENESIS, SITE-DIRECTED; NUCLEIC ACID DENATURATION; POLYMERASE CHAIN REACTION; TEMPERATURE;

EID: 27144490344     PISSN: 03051048     EISSN: 13624962     Source Type: Journal    
DOI: 10.1093/nar/gni115     Document Type: Article

References (6)

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4. Ke,S.H. and Madison,E.L. (1997) Rapid and efficient site-directed mutagenesis by single-tube 'megaprimer' PCR method. Nucleic Acids Res., 25, 3371-3372.
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