Using the Cre/lox system for targeted integration into the human genome: LoxFAS-loxP pairing and delayed introduction of Cre DNA improve gene swapping efficiency

Biotechnology Journal

Volumn 7, Issue 7, 2012, Pages 898-908

  Lanza, Amanda M ^a   Dyess, Timothy J ^a   Alper, Hal S ^a,b  

^a The University of Texas at Austin   (United States)

^b UNIVERSITY OF TEXAS AT AUSTIN   (United States)

Author keywords

Cre recombinase; Genome editing; HT1080; loxFAS; Recombination mediated cassette exchange

Indexed keywords

BEST CHOICE; CRE RECOMBINASE; EDITING TOOLS; HT-1080; HUMAN CELL LINES; HUMAN GENOMES; INTEGRASE; LOXFAS; MAMMALIAN GENOMES; MAMMALIAN SYSTEMS; RATE LIMITING; RECOMBINATION EVENTS; SITE-SPECIFIC; TRANSGENICS; WILD TYPES;

CELL CULTURE; DNA; EFFICIENCY; MAMMALS;

GENES;

CRE RECOMBINASE; FAS ANTIGEN; INTEGRASE; LOXP PROTEIN; PROTEIN; RECOMBINASE; UNCLASSIFIED DRUG;

ARTICLE; CATALYSIS; DNA DEGRADATION; DNA TRANSFECTION; GENE TARGETING; GENOME; HUMAN; HUMAN CELL; IN VITRO STUDY; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN MODIFICATION; WILD TYPE;

BACTERIOPHAGES; BASE SEQUENCE; CELL LINE, TUMOR; DNA; GENETIC ENGINEERING; GENETIC VECTORS; GENOME, HUMAN; HUMANS; INTEGRASES; MOLECULAR SEQUENCE DATA; MUTATION; PLASMIDS; RECOMBINATION, GENETIC; TRANSFECTION;

MAMMALIA;

EID: 84863320643     PISSN: 18606768     EISSN: 18607314     Source Type: Journal    
DOI: 10.1002/biot.201200034     Document Type: Article

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