Cre recombinase-mediated site-specific modification of a cellular genome using an integrase-defective retroviral vector

Biotechnology and Bioengineering

Volumn 107, Issue 4, 2010, Pages 717-729

  Huang, Shuohao ^a   Kawabe, Yoshinori ^a   Ito, Akira ^a   Kamihira, Masamichi ^a  

^a KYUSHU UNIVERSITY   (Japan)

Author keywords

Cre loxP; Integrase defective retroviral vectors; Recombinase mediated cassette exchange

Indexed keywords

CELL LINES; CELLULAR GENOMES; CRE RECOMBINASE; CRE/LOXP; GREEN FLUORESCENT PROTEIN; HYGROMYCIN RESISTANCE; INFECTED CELLS; INTEGRASE; MARKER GENES; MUTANT LOXP; NEOMYCIN RESISTANCE; NONVIRAL; PCR ANALYSIS; RECOMBINASE-MEDIATED CASSETTE EXCHANGE; RED FLUORESCENT PROTEINS; RETROVIRAL VECTORS; SINGLE MUTATION; SITE-SPECIFIC MODIFICATIONS; SITE-SPECIFIC RECOMBINATION; SOUTHERN BLOT; WILD TYPES;

CELL CULTURE; FLUORESCENCE; MACHINERY; ONCOGENIC VIRUSES; VECTORS;

GENE EXPRESSION;

COMPLEMENTARY DNA; CRE RECOMBINASE; GREEN FLUORESCENT PROTEIN; HYGROMYCIN; INTEGRASE; PLASMID VECTOR; RED FLUORESCENT PROTEIN; RETROVIRUS VECTOR;

ARTICLE; CELL LINE; CHROMOSOME; CODON; CONTROLLED STUDY; FLUORESCENCE; GENE CASSETTE; GENE MUTATION; GENETIC RECOMBINATION; IN VITRO STUDY; IN VIVO STUDY; LOXP SITE; MARKER GENE; POLYMERASE CHAIN REACTION; PROMOTER REGION; SOUTHERN BLOTTING; WILD TYPE;

BLOTTING, SOUTHERN; CINNAMATES; CODON, INITIATOR; DRUG RESISTANCE; GENE TARGETING; GENES, REPORTER; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; HYGROMYCIN B; INTEGRASES; LUMINESCENT PROTEINS; NEOMYCIN; POLYMERASE CHAIN REACTION; PROMOTER REGIONS, GENETIC; RECOMBINATION, GENETIC; RETROVIRIDAE;

EID: 78149289055     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.22863     Document Type: Article

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