Codon optimization, expression and enzymatic comparison of Rhizopus oryzae lipases pro-ROL and m-ROL in Pichia pastoris

Shengwu Gongcheng Xuebao/Chinese Journal of Biotechnology

Volumn 27, Issue 12, 2011, Pages 1780-1788

  Yang, Jiangke ^a   Yan, Xiangxiang ^a   Huang, Ribo ^b   Zhang, Bo ^b  

^a WUHAN POLYTECHNIC UNIVERSITY   (China)

^b Guangxi Academy of Science   (China)

Author keywords

m ROL; Overlap extension PCR; Pichia pastoris; pro ROL; Rhizopus oryzae lipases

Indexed keywords

ENZYME PRECURSOR; RECOMBINANT PROTEIN; TRIACYLGLYCEROL LIPASE;

ARTICLE; BIOSYNTHESIS; CHEMISTRY; CODON; COMPARATIVE STUDY; ENZYME SPECIFICITY; ENZYME STABILITY; ENZYMOLOGY; GENETICS; METABOLISM; METHODOLOGY; PICHIA; PROTEIN ENGINEERING; PROTEIN FOLDING; RHIZOPUS;

CODON; ENZYME PRECURSORS; ENZYME STABILITY; LIPASE; PICHIA; PROTEIN ENGINEERING; PROTEIN FOLDING; RECOMBINANT PROTEINS; RHIZOPUS; SUBSTRATE SPECIFICITY;

PICHIA PASTORIS; RHIZOPUS ORYZAE;

EID: 84863290855     PISSN: 10003061     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (25)

1. Gotor-Fernández V, Brieva R, Gotor V. Lipases: useful biocatalysts for the preparation of pharmaceuticals. J Mol Catal B: Enzym, 2006, 40(3/4): 111-120.
2. Fukuda H, Hama S, Tamalampudi S, et al. Whole-cell biocatalysts for biodiesel fuel production. Trends Biotechnol, 2008, 26(12): 668-673.
3. Tamalampudi S, Talukder MR, Hama S, et al. Enzymatic production of biodiesel from Jatropha oil: a comparative study of immobilized-whole cell and commercial lipases as a biocatalyst. Biochem Eng J, 2008, 39(1): 185-189.
4. Beer HD, Wohlfahrt G, Schmid RD, et al. The folding and activity of the extracellular lipase of Rhizopus oryzae are modulated by a prosequence. Biochem J, 1996, 319(Pt 2): 351-359.
5. Shinde U, Inouye M. Folding mediated by an intramolecular chaperone: autoprocessing pathway of the precursor resolved via a substrate assisted catalysis mechanism. J Mol Biol, 1995, 247(3): 390-395.
6. Chen YJ, Inouye M. The intramolecular chaperone- mediated protein folding. Curr Opin Stru Biol, 2008, 18(6): 765-770.
7. Beer HD, McCarthy JEG, Bornscheuer UT, et al. Cloning, expression, characterization and role of the leader sequence of a lipase from Rhizopus oryzae. Gene Struct Expr, 1998, 1399(2/3): 173-180.
8. Takahashi S, Ueda M, Tanaka A. Function of the prosequence for the in vivo folding and secretion of active Rhizopus oryzae lipase in Saccharomyces cerevisiae. Appl Microbiol Biotechnol, 2001, 55(4): 454-462.
9. Ueda M, Takahashi S, Washida M, et al. Expression of Rhizopus oryzae lipase gene in Saccharomyces cerevisiae. J Mol Catal B: Enzym, 2002, 17(3/5): 113-124.
10. Minning S, Schmidt-Dannert C, Schmid RD. Functional expression of Rhizopus oryzae lipase in Pichia pastoris: high-level production and some properties. J Biotechnol, 1998, 66(2/3): 147-156.
11. Cos O, Resina D, Ferrer P, et al. Heterologous production of Rhizopus oryzae lipase in Pichia pastoris using the alcohol oxidase and formaldehyde dehydrogenase promoters in batch and fed-batch cultures. Biochem Engin J, 2005, 26(2/3): 86-94.
12. Fernández L, Pérez-Victoria I, Zafra A, et al. High-level expression and characterization of Galactomyces geotrichum (BT107) lipase I in Pichia pastoris. Protein Expr Purif, 2006, 49(2): 256-264.
13. Shu ZY, Duan MJ, Yang JK, et al. Aspergillus niger lipase: heterologous expression in Pichia pastoris, molecular modeling prediction and the importance of the hinge domains at both sides of the lid domain to interfacial activation. Biotechnol Prog, 2009, 25(2): 409-416.
14. Yang JK, Zhang B, Yan YJ. Cloning and expression of Pseudomonas fluorescens 26-2 lipase gene in Pichia pastoris and characterizing for transesterification. Appl Biochem Biotechnol, 2009, 159(2): 355-365.
15. Müller M. Codon optimization of papillomavirus genes. Methods Mol Med, 2005, 119: 433-444.
16. Tokuoka M, Tanaka M, Ono K, et al. Codon optimization increases steady-state mRNA levels in Aspergillus oryzae heterologous gene expression. Appl Environ Microbiol, 2008, 74(21): 6538-6546.
17. Daniell H, Ruiz G, Denes B, et al. Optimization of codon composition and regulatory elements for expression of human insulin like growth factor-1 in transgenic chloroplasts and evaluation of structural identity and function. BMC Biotechnol, 2009, 9(1): 33.
18. Kouker G, Jaeger KE. Specific and sensitive plate assay for bacterial lipases. Appl Environ Microbiol, 1987, 53(1): 211-213.
19. Kumar S, Tamura K, Nei M. MEGA3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform, 2004, 5(2): 150-163.
20. Ma BY, Tsai CJ, Nussinov R. Binding and folding: in search of intramolecular chaperone-like building block fragments. Protein Eng Des Sel, 2000, 13(9): 617-627.
21. Chapus C, Semeriva M, Bovier-Lapierre C, et al. Mechanism of pancreatic lipase action. 1. Interfacial activation of pancreatic lipase. Biochemistry, 1976, 15(23): 4980-4987.
22. Surribas A, Stahn R, Montesinos JL, et al. Production of a Rhizopus oryzae lipase from Pichia pastoris using alternative operational strategies. J Biotechnol, 2007, 130(3): 291-299.
23. Ramchuran SO, Vargas VA, Hatti-Kaul R, et al. Production of a lipolytic enzyme originating from Bacillus halodurans LBB2 in the methylotrophic yeast Pichia pastoris. Appl Microbial Biotechnol, 2006, 71(4): 463-472.
24. Yao HY, Yu SW, Zhang LD, et al. Isolation of a novel lipase gene from Serratia liquefaciens S33 DB-1, functional expression in Pichia pastoris and its properties. Mol Biotechnol, 2008, 38(2): 99-107.
25. Zor T, Selinger Z. Linearization of the Bradford protein assay increases its sensitivity: theoretical and experimental studies. Anal Biochem, 1996, 236: 302-308.