Functional expression of Rhizopus oryzae lipase in Pichia pastoris: High-level production and some properties

Journal of Biotechnology

Volumn 66, Issue 2-3, 1998, Pages 147-156

  Minning, Stefan ^a   Schmidt Dannert, Claudia ^a   Schmid, Rolf D ^a  

^a UNIVERSITY OF STUTTGART   (Germany)

Author keywords

Lipase; Pichia pastoris; Production; Properties; Purification; Rhizopus oryzae

Indexed keywords

BATCH CELL CULTURE; CATALYST ACTIVITY; FUNGI; GLYCEROL; METHANOL; PURIFICATION; YEAST;

LIPASE;

ENZYMES;

GLYCEROL; METHANOL; RECOMBINANT DNA; RECOMBINANT ENZYME; SIGNAL PEPTIDE; TRIACYLGLYCEROL; TRIACYLGLYCEROL LIPASE;

ARTICLE; BIOTECHNOLOGY; CONTROLLED STUDY; DNA RECOMBINATION; ENZYME GLYCOSYLATION; ENZYME PURIFICATION; FERMENTATION; ISOELECTRIC FOCUSING; MOLECULAR CLONING; NONHUMAN; PLASMID; PRIORITY JOURNAL; PROTEIN SYNTHESIS REGULATION; RHIZOPUS; TEMPERATURE; YEAST;

ESCHERICHIA COLI; FERMENTATION; INCLUSION BODIES; LIPASE; PICHIA; PROTEIN ENGINEERING; PROTEIN FOLDING; RECOMBINANT PROTEINS; RHIZOPUS; SELECTION (GENETICS); SUBSTRATE SPECIFICITY; TEMPERATURE; TRANSFORMATION, GENETIC;

EID: 0344404412     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0168-1656(98)00142-4     Document Type: Article

References (26)

1. Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Smith, J.A., Struhlo, K., 1987. Current Protocols in Molecular Biology. New York, Greene Publishing Associates.
2. Beer, H.D., 1995. Untersuchung der Lipase aus Rhizopus oryzae auf molekularer Ebene: Das Gen, der Reaktionsmechanismus und die Funktion der Prosequenz, Ph.D. Thesis, University of Hannover.
3. Beer H.D., Wohlfahrt G., Schmid R.D., McCarthy J.E. The folding and activity of the extracellular lipase of Rhizopus oryzae are modulated by a prosequence. Biochem. J. 319:1996;351-359.
4. Ben Salah A., Fendri K., Gargoury Y. La lipase de Rhizopus oryzae: production, purification et charactéristiques biochimiques. Rev. Fr. Corps Gras. 41:1994;133-137.
5. Björkling F., Godtfredsen S.E., Kirk O. The future impact of industrial lipases. Trends Biotechnol. 9:1991;360-363.
6. Bornscheuer, U.T., Pleiss, J., Schmidt-Dannert, C., Schmid, R.D., 1998. Lipases from Rhizopus species: genetics, structure and applications. In: Alberghina, H. (Ed.), Protein Engineering in Industrial Biotechnology, Harwood, London, in press.
7. Bornscheuer U.T. Lipase-catalyzed synthesis of monoacylglycerols. Enzyme. Microb. Technol. 17:1995;578-586.
8. Butcher L.A., Tomkins J.H. Silver staining method useful for ultrathin polyacrylamid gels on support films. Anal. Biochem. 148:1986;384-388.
9. Catoni E., Schmidt-Dannert C., Brocca S., Schmid R.D. Overexpression of lipase A and B of Geotrichum candidum in Pichia pastoris: High-level production and some properties of functional expressed lipase B. Biotechniques. 11:1997;689-695.
10. Cregg J.M., Vedvick T.S., Raschke W.C. Recent advances in the expression of foreign genes in Pichia pastoris. Biotechnology. 11:1993;905-910.
11. Haalck L., Paltauf F., Pleiss J., Schmid R.D., Spener F., Stadler P. Stereoselectivity of lipase from Rhizopus oryzae towards triacylglycerols and analogs: computer-aided modeling and experimential validation. Methods Enzymol. 184:1997;353-376.
12. Haas M.J., Cichowicz D.J., Bailey D.G. Purification and characterization of an extracellular lipase from the fungus Rhizopus delemar. Lipids. 27:1992;571-576.
13. Haas, M.J., Joerger, R.D., 1995. Lipases of the genera Rhizopus and Rhizomucor: versatile catalysts in nature and the laboratory. In: Khachatourians, G.G., Hui, Y.H. (Eds.), Food Biotechnology: Microorganisms. VCH, Weinheim, pp. 549-588.
14. Jiménez R.E., Sánchez K., Roca H., Delgado J.M. Different methanol feeding strategies to recombinant Pichia pastoris cultures producing high level of dextranase. Biotechniques. 11:1997;461-466.
15. Joerger R.D., Haas M.J. Overexpression of a Rhizopus delemar lipase gene in Escherichia coli. Lipids. 28:1993;81-88.
16. Joerger R.D., Haas M.J. Alteration of chain length selectivity of a Rhizopus delemar lipase through site-directed mutagenesis. Lipids. 29:1994;377-384.
17. Kohno M., Kugimiya W., Hashimoto Y., Morita Y. Purification, characterization, and crystallization of two types of lipase from Rhizopus niveus. Biosci. Biotechnol. Biochem. 58:1994;1007-1012.
18. Salleh A.B., Musani M., Yunus K., Ampon W.M.Z., Razak C.N.A. Extra- and intra-cellular lipases from a thermophilic Rhizopus oryzae and factors affecting their production. Can. J. Microbiol. 39:1993;978-981.
19. Sambroock, J., Fritsch, E.F., Maniatis, T., 1989. Molecular cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
20. Scorer C.A., Buckholz R.G., Clare J.J., Romanos M.A. The intracellular production and secretion of HIV-1 envelope protein in the methylotrophic yeast Pichia pastoris. Gene. 136:1993;111-119.
21. Schipper M.A.A. A revision of the genus Rhizopus II. The Rhizopus stolonifer-group and Rhizopus oryzae. Stud. Mycol. 25:1984;1-19.
22. Schmid R.D., Verger R. Lipases, interfacial enzymes with attractive applications. Agnew. Chem. Int. Ed. Engl. 37:1998;1608-1633.
23. Shimada Y., Sugihara A., Nakano H., Nagao T., Suenaga M., Nakai S., Tominaga Y. Fatty acid specificity of Rhizopus delemar lipase in acidolysis. J. Ferment. Bioeng. 83:1997;321-327.
24. Sreekrishna, K., Kropp, K.E., 1996. Pichia pastoris. In: Wolf, K. (Ed.), Nonconventional Yeasts in Biotechnology - A Handbook. Springer Verlag, Berlin, pp. 203-250.
25. Storme V., de Meutter J., Messens J., Lauwereys M., Laroche Y. High-level secretion and very efficient isotopic labeling of tick anticoagulant peptide (TAP) expressed in the methylotropic yeast Pichia pastoris. Biotechnology. 12:1994;1119-1124.
26. Uyttenbroeck W., Hendriks D., Vriend G., de Baere I., Moens L., Scharpé S. Molecular characterization of an extra acid-resistant lipase produced by Rhizopus javanicus. Biol. Chem. Hoppe-Seyler. 374:1993;245-254.