Function of the prosequence for in vivo folding and secretion of active Rhizopus oryzae lipase in Saccharomyces cerevisiae

Applied Microbiology and Biotechnology

Volumn 55, Issue 4, 2001, Pages 454-462

  Takahashi, S ^a   Ueda, M ^a   Tanaka, A ^a  

^a KYOTO UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

CHAPERONE; ENZYME PRECURSOR; TRIACYLGLYCEROL LIPASE;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; CIS TRANS ISOMERISM; CONTROLLED STUDY; ENZYME RELEASE; GENE DELETION; GENE EXPRESSION SYSTEM; GENE FUNCTION; NONHUMAN; PROTEIN FOLDING; RHIZOPUS ORYZAE; SACCHAROMYCES CEREVISIAE; STRUCTURE ACTIVITY RELATION;

AMINO ACID SEQUENCE; BASE SEQUENCE; DNA PRIMERS; LIPASE; MOLECULAR SEQUENCE DATA; PLASMIDS; PROTEIN FOLDING; RHIZOPUS; SACCHAROMYCES CEREVISIAE; SEQUENCE DELETION; SEQUENCE HOMOLOGY, AMINO ACID;

RHIZOPUS ORYZAE; SACCHAROMYCES CEREVISIAE;

EID: 0035003618     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s002530000537     Document Type: Article

References (42)

1. The folding and activity of the extracellular lipase of Rhizopus oryzae are modulated by a prosequence
2. Cloning, expression, characterization and role of the leader sequence of a lipase from Rhizopus oryzae
3. Rhizomucor miehei triglyceride lipase is synthesized as a precursor
4. Glycosylation and structure of the yeast MFα1 α-factor precursor is important for efficient transport through the secretory pathway
5. Prediction of the secondary structure of proteins from their amino acid sequence
6. Insulin biosynthesis in the rat: Demonstration of two proinsulins
7. Role of the lipB gene product in the folding of the secreted lipase of Pseudomonas glumae
8. Requirement for activin A and transforming growth factor-β-1 pro-region in homodimer assembly
9. Cloning, expression and characterization of a cDNA encoding a lipase from Rhizopus delemar
10. Activation of a bacterial lipase by its chaperone
11. Lipase modulator protein (LimL) of Pseudomonas sp. strain 109
12. Role of the gene encoding lipase activator from Pseudomonas sp. strain KWI-56 in in vitro activation of lipase
13. Intramolecular chaperone: The role of the pro-peptide in protein folding
14. Transformation of intact yeast cells treated with alkali cations
15. A general method for introducing a series of mutations into cloned DNA using the polymerase chain reaction
16. Cloning, sequence, and expression of a lipase gene from Pseudomonas cepacia: Lipase production in heterologous hosts requires two Pseudomonas genes
17. A novel heterologous gene expression system in Saccharomyces cerevisiae using the isocitrate lyase gene promoter from Candida tropicalis
18. Functional analysis of the intramolecular chaperone. Mutational hot spots in the subtilisin pro-peptide and a second-site suppressor mutation within the sub-tilisin molecule
19. Purification, characterization, and crystallization of two types of lipase from Rhizopus niveus
20. A novel and simple colorimetric assay for human serum lipase
21. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
22. A non-covalent NH2-terminal pro-region aids the production of active aqualysin I (a thermophilic protease) without the COOH-terminal pro-sequence in Escherichia coli
23. Degradation from the endoplasmic reticulum: Disposing of newly synthesized proteins
24. Functional expression of Rhizopus oryzae lipase in Pichia pastoris: High-level production and some properties
25. Cloning and nucleotide sequence of cDNA encoding a lipase from Fusarium heterosporum
26. Proteolytic processing and physiological regulation
27. Unity in function in the absence of consensus in sequence: Role of leader peptides in export
28. Cloning, sequencing, and heterologous expression of a gene coding for Arthromyces ramosus peroxidase
29. Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa
30. Intramolecular chaperones and protein folding
31. The α-lytic protease pro-region does not require a physical linkage to activate the protease domain in vivo
32. NGF/BDNF chimeric proteins: Analysis of neuro-trophin specificity by homolog-scanning mutagenesis
33. Primary structure of the Saccharomyces cerevisiae GAL7 gene
34. Extracellular production of active Rhizopus oryzae lipase by Saccharomyces cerevisiae
35. Independent production of two molecular forms of a recombinant Rhizopus oryzae lipase by KEX2-engineered strains of Saccharomyces cerevisiae
36. Protein glycosylation in yeast
37. Induction and subcellular localization of enzymes participating in propionate metabolism in Candida tropicalis
38. Biogenesis of von Willebrand factor-containing organelles in heterologous transfected CV-1 cells
39. The pro region of BPTI facilitates folding
40. Propeptide of carboxypeptidase Y provides a chaperone-like function as well as inhibition of the enzymatic activity
41. Refolding of a carboxypeptidase Y folding intermediate in vitro by low-affinity binding of the proregion
42. Pro-sequence of subtilisin can guide the refolding of denatured subtilisin in an intermolecular process