Rapid detection of rpoB mutations in rifampin resistant M. Tuberculosis from sputum samples by denaturing gradient gel electrophoresis

International Journal of Medical Sciences

Volumn 9, Issue 2, 2012, Pages 148-156

  Li, Jun ^a   Xin, Jiaojiao ^a   Zhang, Liyuan ^a   Jiang, Longyan ^a   Cao, Hongcui ^a   Li, Lanjuan ^a  

^a ZHEJIANG UNIVERSITY   (China)

Author keywords

Genotyping; Microbial drug resistance; Mycobacterium tuberculosis; Resistance

Indexed keywords

ETHAMBUTOL; ISONIAZID; RIFAMPICIN; STREPTOMYCIN;

ACID FAST BACTERIUM; AMINO ACID SUBSTITUTION; ANTIBIOTIC RESISTANCE; ANTIBIOTIC SENSITIVITY; ARTICLE; BACTERIAL STRAIN; BACTERIUM CULTURE; BACTERIUM DETECTION; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATE; CONTROLLED STUDY; DENATURING GRADIENT GEL ELECTROPHORESIS; DNA SEQUENCE; GENE IDENTIFICATION; MARKER GENE; MUTATIONAL ANALYSIS; MYCOBACTERIUM TUBERCULOSIS; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; RPOB GENE; SPUTUM ANALYSIS; STAINING; WILD TYPE;

BACTERIAL PROTEINS; DENATURING GRADIENT GEL ELECTROPHORESIS; DNA MUTATIONAL ANALYSIS; DNA, BACTERIAL; DRUG RESISTANCE, MICROBIAL; HUMANS; MICROBIAL SENSITIVITY TESTS; MUTATION; MYCOBACTERIUM TUBERCULOSIS; POLYMERASE CHAIN REACTION; RIFAMPIN; SPUTUM;

EID: 84863060912     PISSN: 14491907     EISSN: None     Source Type: Journal    
DOI: 10.7150/ijms.3605     Document Type: Article

References (38)

1. Marin M, Garcia de Viedma D, Ruiz-Serrano MJ, Bouza E. Rapid direct detection of multiple rifampin and isoniazid resistance mutations in Mycobacterium tuberculosis in respiratory samples by real-time PCR. Antimicrob Agents Chemother 2004;48:4293-4300.
2. Viveiros M, Amaral L. Enhancement of antibiotic activity against poly-drug resistant Mycobacterium tuberculosis by phenothiazines. Int J Antimicrob Agents 2001;17:225-228.
3. Zhang SL, Shen JG, Xu PH, Li DX, Sun ZQ, Li L, Yang ZR, et al. A novel genotypic test for rapid detection of multidrug-resistant Mycobacterium tuberculosis isolates by a multiplex probe array. J Appl Microbiol 2007;103:1262-1271.
4. Pfyffer GE, Bonato DA, Ebrahimzadeh A, Gross W, Hotaling J, Kornblum J, Laszlo A, et al. Multicenter laboratory validation of susceptibility testing of Mycobacterium tuberculosis against classical second-line and newer antimicrobial drugs by using the radiometric BACTEC 460 technique and the proportion method with solid media. J Clin Microbiol 1999;37:3179-3186.
5. Kim SJ. Drug-susceptibility testing in tuberculosis: methods and reliability of results. Eur Respir J 2005;25:564-569.
6. McCammon MT, Gillette JS, Thomas DP, Ramaswamy SV, Graviss EA, Kreiswirth BN, Vijg J, et al. Detection of rpoB mutations associated with rifampin resistance in Mycobacterium tuberculosis using denaturing gradient gel electrophoresis. Antimicrob Agents Chemother 2005;49:2200-2209.
7. Sheng J, Li J, Sheng G, Yu H, Huang H, Cao H, Lu Y, et al. Characterization of rpoB mutations associated with rifampin resistance in Mycobacterium tuberculosis from eastern China. J Appl Microbiol 2008;105:904-911.
8. Ahmad S, Mokaddas E, Fares E. Characterization of rpoB mutations in rifampin-resistant clinical Mycobacterium tuberculosis isolates from Kuwait and Dubai. Diagn Microbiol Infect Dis 2002;44:245-252.
9. Spindola de Miranda S, Kritski A, Filliol I, Mabilat C, Panteix G, Drouet E. Mutations in the rpoB gene of rifampicin-resistant Mycobacterium tuberculosis strains isolated in Brazil and France. Mem Inst Oswaldo Cruz 2001;96:247-250.
10. Khan K, Muennig P, Behta M, Zivin JG. Global drug-resistance patterns and the management of latent tuberculosis infection in immigrants to the United States. N Engl J Med 2002;347:1850-1859.
11. Lee AS, Lim IH, Tang LL, Wong SY. High frequency of mutations in the rpoB gene in rifampin-resistant clinical isolates of Mycobacterium tuberculosis from Singapore. J Clin Microbiol 2005;43:2026-2027.
12. Ramaswamy S, Musser JM. Molecular genetic basis of antimicrobial agent resistance in Mycobacterium tuberculosis: 1998 update. Tuber Lung Dis 1998;79:3-29.
13. Patnaik M, Liegmann K, Peter JB. Rapid detection of smear-negative Mycobacterium tuberculosis by PCR and sequencing for rifampin resistance with DNA extracted directly from slides. J Clin Microbiol 2001;39:51-52.
14. Johansen IS, Thomsen VO, Marjamaki M, Sosnovskaja A, Lundgren B. Rapid, automated, nonradiometric susceptibility testing of Mycobacterium tuberculosis complex to four first-line antituberculous drugs used in standard short-course chemotherapy. Diagn Microbiol Infect Dis 2004;50:103-107.
15. Rohner P, Ninet B, Benri AM, Auckenthaler R. Evaluation of the Bactec 960 automated nonradiometric system for isolation of mycobacteria from clinical specimens. Eur J Clin Microbiol Infect Dis 2000;19:715-717.
16. Kim S, Park EM, Kwon OJ, Lee JH, Ki CS, Lee NY, Koh WJ. Direct application of the PCR restriction analysis method for identifying NTM species in AFB smear-positive respiratory specimens. Int J Tuberc Lung Dis 2008;12:1344-1346.
17. Huang HJ, Xiang DR, Sheng JF, Li J, Pan XP, Yu HY, Sheng GP, et al. RpoB nested PCR and sequencing for the early diagnosis of tuberculous meningitis and rifampicin resistance. Int J Tuberc Lung Dis 2009;13:749-754.
18. De Angelis M, Di Cagno R, Gallo G, Curci M, Siragusa S, Crecchio C, Parente E, et al. Molecular and functional characterization of Lactobacillus sanfranciscensis strains isolated from sourdoughs. Int J Food Microbiol 2007;114:69-82.
19. Spano G, Lonvaud-Funel A, Claisse O, Massa S. In Vivo PCR-DGGE analysis of Lactobacillus plantarum and Oenococcus oeni populations in red wine. Curr Microbiol 2007;54:9-13.
20. da Mota FF, Gomes EA, Paiva E, Seldin L. Assessment of the diversity of Paenibacillus species in environmental samples by a novel rpoB-based PCR-DGGE method. FEMS Microbiol Ecol 2005;53:317-328.
21. Taylor MW, Schupp PJ, Dahllof I, Kjelleberg S, Steinberg PD. Host specificity in marine sponge-associated bacteria, and potential implications for marine microbial diversity. Environ Microbiol 2004;6:121-130.
22. Hori T, Haruta S, Ueno Y, Ishii M, Igarashi Y. Direct comparison of single-strand conformation polymorphism (SSCP) and denaturing gradient gel electrophoresis (DGGE) to characterize a microbial community on the basis of 16S rRNA gene fragments. J Microbiol Methods 2006;66:165-169.
23. Strauss J, White A, Ambrose C, McDonald J, Allen-Vercoe E. Phenotypic and genotypic analyses of clinical Fusobacterium nucleatum and Fusobacterium periodonticum isolates from the human gut. Anaerobe 2008;14:301-309.
24. Scarpellini P, Braglia S, Carrera P, Cedri M, Cichero P, Colombo A, Crucianelli R, et al. Detection of rifampin resistance in Mycobacterium tuberculosis by double gradient-denaturing gradient gel electrophoresis. Antimicrob Agents Chemother 1999;43:2550-2554.
25. Yam WC, Tam CM, Leung CC, Tong HL, Chan KH, Leung ET, Wong KC, et al. Direct detection of rifampin-resistant mycobacterium tuberculosis in respiratory specimens by PCR-DNA sequencing. J Clin Microbiol 2004;42:4438-4443.
26. Di Perri G, Bonora S. Which agents should we use for the treatment of multidrug-resistant Mycobacterium tuberculosis? J Antimicrob Chemother 2004;54:593-602.
27. Wu TL, Chia JH, Kuo AJ, Su LH, Wu TS, Lai HC. Rapid identification of mycobacteria from smear-positive sputum samples by nested PCR-restriction fragment length polymorphism analysis. J Clin Microbiol 2008;46:3591-3594.
28. Chaves F, Alonso-Sanz M, Rebollo MJ, Tercero JC, Jimenez MS, Noriega AR. RpoB mutations as an epidemiologic marker in rifampin-resistant Mycobacterium tuberculosis. Int J Tuberc Lung Dis 2000;4:765-770.
29. Heep M, Brandstatter B, Rieger U, Lehn N, Richter E, Rusch-Gerdes S, Niemann S. Frequency of rpoB mutations inside and outside the cluster I region in rifampin-resistant clinical Mycobacterium tuberculosis isolates. J Clin Microbiol 2001;39:107-110.
30. Klein JL, Brown TJ, French GL. Rifampin resistance in Mycobacterium kansasii is associated with rpoB mutations. Antimicrob Agents Chemother 2001;45:3056-3058.
31. Torres MJ, Criado A, Gonzalez N, Palomares JC, Aznar J. Rifampin and isoniazid resistance associated mutations in Mycobacterium tuberculosis clinical isolates in Seville, Spain. Int J Tuberc Lung Dis 2002;6:160-163.
32. Cavusoglu C, Hilmioglu S, Guneri S, Bilgic A. Characterization of rpoB mutations in rifampin-resistant clinical isolates of Mycobacterium tuberculosis from Turkey by DNA sequencing and line probe assay. J Clin Microbiol 2002;40:4435-4438.
33. Agdamag DM, Kageyama S, Solante R, Espantaleon AS, Sangco JC, Suzuki Y. Characterization of clinical isolates of Mycobacterium tuberculosis resistant to drugs and detection of RpoB mutation in multidrug-resistant tuberculosis in the Philippines. Int J Tuberc Lung Dis 2003;7:1104-1108.
34. Prammananan T, Cheunoy W, Taechamahapun D, Yorsangsukkamol J, Phunpruch S, Phdarat P, Leechawengwong M, et al. Distribution of rpoB mutations among multidrug-resistant Mycobacterium tuberculosis (MDRTB) strains from Thailand and development of a rapid method for mutation detection. Clin Microbiol Infect 2008;14:446-453.
35. O'Riordan P, Schwab U, Logan S, Cooke G, Wilkinson RJ, Davidson RN, Bassett P, et al. Rapid molecular detection of rifampicin resistance facilitates early diagnosis and treatment of multi-drug resistant tuberculosis: case control study. PLoS One 2008;3:e3173.
36. McBain AJ, Bartolo RG, Catrenich CE, Charbonneau D, Ledder RG, Gilbert P. Effects of triclosan-containing rinse on the dynamics and antimicrobial susceptibility of in vitro plaque ecosystems. Antimicrob Agents Chemother 2003;47:3531-3538.
37. McCammon MT, Gillette JS, Thomas DP, Ramaswamy SV, Rosas II, Graviss EA, Vijg J, et al. Detection by denaturing gradient gel electrophoresis of pncA mutations associated with pyrazinamide resistance in Mycobacterium tuberculosis isolates from the United States-Mexico border region. Antimicrob Agents Chemother 2005;49:2210-2217.
38. Tang X, Morris SL, Langone JJ, Bockstahler LE. Microarray and allele specific PCR detection of point mutations in Mycobacterium tuberculosis genes associated with drug resistance. J Microbiol Methods 2005;63:318-330.