rpoB nested PCR and sequencing for the early diagnosis of tuberculous meningitis and rifampicin resistance

International Journal of Tuberculosis and Lung Disease

Volumn 13, Issue 6, 2009, Pages 749-754

  Huang, H J ^a   Xiang, D R ^a   Sheng, J F ^a   Li, J ^a   Pan, X P ^a   Yu, H Y ^a   Sheng, G P ^a   Li, Lan Juan ^a  

^a ZHEJIANG UNIVERSITY   (China)

Author keywords

Diagnosis; rpoB gene; Sensitivity; Specificity; Tuberculous meningitis

Indexed keywords

RIFAMPICIN; RNA POLYMERASE BETA SUBUNIT;

ADOLESCENT; ADULT; AGED; ARTICLE; BACTERIAL STRAIN; CEREBROSPINAL FLUID; CLINICAL ARTICLE; CODON; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; EARLY DIAGNOSIS; FEMALE; GENBANK; HOSPITAL BASED CASE CONTROL STUDY; HUMAN; MALE; MUTATION; MYCOBACTERIUM TUBERCULOSIS; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RETROSPECTIVE STUDY; SENSITIVITY AND SPECIFICITY; SEQUENCE ANALYSIS; TUBERCULOUS MENINGITIS;

ADOLESCENT; ADULT; AGED; ANTITUBERCULAR AGENTS; BACTERIAL PROTEINS; CEREBROSPINAL FLUID; DNA, BACTERIAL; DRUG RESISTANCE, BACTERIAL; EARLY DIAGNOSIS; FEMALE; HUMANS; MALE; MIDDLE AGED; MUTATION; MYCOBACTERIUM TUBERCULOSIS; POLYMERASE CHAIN REACTION; RETROSPECTIVE STUDIES; RIFAMPIN; SENSITIVITY AND SPECIFICITY; SINGLE-BLIND METHOD; TUBERCULOSIS, MENINGEAL; YOUNG ADULT;

EID: 67649227424     PISSN: 10273719     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (13)

1. Desai D, Nataraj G, Kulkarni S, et al. Utility of the polymerase chain reaction in the diagnosis of tuberculous meningitis. Res Microbiol 2006; 157: 967-970. (Pubitemid 44828461)
2. Takahashi T, Nakayama T, Tamura M, et al. Nested polymerase chain reaction for assessing the clinical course of tuberculous meningitis. Neurology 2005; 64: 1789-1793. (Pubitemid 40695821)
3. Scarpellini P, Racca S, Cinque P, et al. Nested polymerase chain reaction for diagnosis and monitoring treatment response in AIDS patients with tuberculous meningitis. AIDS 1995; 9: 895-900.
4. Rafi W, Venkataswamy M M, Ravi V, Chandramuki A. Rapid diagnosis of tuberculous meningitis: a comparative evaluation of in-house PCR assays involving three mycobacterial DNA sequences, IS6110, MPB-64 and 65 kDa antigen. J Neurol Sci 2007; 252: 163-168. (Pubitemid 46048650)
5. Traore H, van Deun A, Shamputa I C, Rigouts L, Portaels F. Direct detection of Mycobacterium tuberculosis complex DNA and rifampicin resistance in clinical specimens from tuberculosis patients by line-probe assay. J Clin Microbiol 2006; 44: 4384-4388.
6. Suresh N, Singh U B, Gupta C, Arora J, Rana T, Samantaray J C. Rapid detection of rifampicin-resistant Mycobacterium tuberculosis directly from stained sputum smears using single-tube nested polymerase chain reaction deoxyribonucleic acid sequencing. Diagn Microbiol Infect Dis 2007; 58: 217-222.
7. Telenti A, Imboden P, Marchesi F, et al. Detection of rifampicin-resistance mutations in Mycobacterium tuberculosis. Lancet 1993; 341: 647-650.
8. Mikhailovich V, Lapa S, Gryadunov D, et al. Identification of rifampicin-resistant Mycobacterium tuberculosis strains by hybridization, PCR and ligase detection reaction on oligonucleotide microchips. J Clin Microbiol 2001; 39: 2531-2540.
9. British Medical Research Council. Streptomycin treatment of tuberculous meningitis. Lancet 1948; 17: 582-596.
10. Sheng J, Li J, Sheng G, et al. Characterization of rpoB mutations associated with rifampicin resistance in Mycobacterium tuberculosis from eastern China. J Appl Microbiol 2008; 105: 904-911.
11. Yun Y J, Lee K H, Haihua L, et al. Detection and identification of Mycobacterium tuberculosis in joint biopsy specimens by rpoB PCR cloning and sequencing. J Clin Microbiol 2005; 43: 174-178.
12. He G X, Zhao Y L, Jiang G L, et al. Prevalence of tuberculosis drug resistance in 10 provinces of China. BMC Infect Dis 2008; 8: 166.
13. Somoskovi A, Parsons L M, Salfinger M. The molecular basis of resistance to isoniazid, rifampicin and pyrazinamide in Mycobacterium tuberculosis. Respir Res 2001; 2: 164-168.