Increasing the productivity of TNFR-Fc in GS-CHO cells at reduced culture temperatures

Biotechnology and Bioprocess Engineering

Volumn 16, Issue 1, 2011, Pages 136-143

  Kou, Tian Ci ^a   Fan, Li ^a   Zhou, Yan ^a   Ye, Zhao Yang ^a   Zhao, Liang ^a   Tan, Wen Song ^a  

^a EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

Author keywords

Cell growth arrest; GS CHO cells; Low temperature; TNFR Fc; Transcriptional level

Indexed keywords

BATCH CULTURE; BENEFICIAL EFFECTS; BIOPROCESS OPTIMIZATION; BIPHASIC; CELL-CYCLE ARREST; CULTIVATION PROCESS; CULTURE TEMPERATURE; FED BATCHES; GROWTH ARREST; INHIBITION EFFECT; LOW CULTURE TEMPERATURE; LOW TEMPERATURE; RT-PCR ANALYSIS; SIALYLATION; TNFR-FC; TRANSCRIPTIONAL LEVELS;

BATCH CELL CULTURE; CELL GROWTH; CELLS; GENES; GROWTH KINETICS; PRODUCTIVITY; TRANSCRIPTION;

LOW TEMPERATURE PRODUCTION;

GLUTAMATE AMMONIA LIGASE; HYBRID PROTEIN; MESSENGER RNA; RECOMBINANT PROTEIN; SIALIC ACID; TUMOR NECROSIS FACTOR RECEPTOR IMMUNOGLOBULIN FC FRAGMENT; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; BATCH CELL CULTURE; CELL CULTURE; CELL GROWTH; CELL METABOLISM; CHO CELL; CONTROLLED STUDY; FED BATCH CULTURE; FEMALE; FUSION GENE; G1 PHASE CELL CYCLE CHECKPOINT; GENE EXPRESSION; GENE IDENTIFICATION; GROWTH INHIBITION; LOW TEMPERATURE; NONHUMAN; PROCESS DEVELOPMENT; PROCESS OPTIMIZATION; PROTEIN SYNTHESIS; REAL TIME POLYMERASE CHAIN REACTION; SIALYLATION; TEMPERATURE SENSITIVITY; TRANSCRIPTION REGULATION; TUMOR NECROSIS FACTOR RECEPTOR IMMUNOGLOBULIN FC FRAGMENT GENE; UPREGULATION;

EID: 84863046077     PISSN: 12268372     EISSN: 19763816     Source Type: Journal    
DOI: 10.1007/s12257-010-0157-1     Document Type: Article

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