Tiling genomes of pathogenic viruses identifies potent antiviral shRNAs and reveals a role for secondary structure in shRNA efficacy

Proceedings of the National Academy of Sciences of the United States of America

Volumn 109, Issue 3, 2012, Pages 869-874

  Tan, Xu ^a   Lu, Zhi John ^b   Gao, Geng ^a   Xu, Qikai ^a   Hu, Long ^b   Fellmann, Christof ^c   Li, Mamie Z ^a   Qu, Hongjing ^a   Lowe, Scott W ^c,d   Hannon, Gregory J ^c,d   Elledge, Stephen J ^a  

^a BRIGHAM AND WOMEN S HOSPITAL   (United States)

^b TSINGHUA UNIVERSITY   (China)

^c Howard Hughes Medical Institute Cold Spring Harbor Laboratory Cold Spring Harbor   (United States)

^d HOWARD HUGHES MEDICAL INSTITUTE   (United States)

Author keywords

RNA secondary structure; shRNA optimization

Indexed keywords

ANTIVIRUS AGENT; GENOMIC RNA; SHORT HAIRPIN RNA;

ANTIVIRAL ACTIVITY; ANTIVIRAL THERAPY; ARTICLE; CD4+ T LYMPHOCYTE; CONTROLLED STUDY; DRUG EFFICACY; DRUG POTENCY; DRUG TARGETING; GENE EXPRESSION; GENE SILENCING; HELA CELL; HEPATITIS C VIRUS; HUMAN; HUMAN CELL; HUMAN IMMUNODEFICIENCY VIRUS; INFLUENZA VIRUS; NONHUMAN; PRIORITY JOURNAL; PROTEIN SECONDARY STRUCTURE; RNA STRUCTURE; SENSOR; VIRUS DNA CELL DNA INTERACTION; VIRUS GENE; VIRUS GENOME;

ANTIVIRAL AGENTS; BASE SEQUENCE; CELLS, CULTURED; GENETIC TESTING; GENOME, VIRAL; HELA CELLS; HEPACIVIRUS; HIV; HUMANS; INFLUENZA A VIRUS; NUCLEIC ACID CONFORMATION; REPRODUCIBILITY OF RESULTS; RNA INTERFERENCE; RNA, SMALL INTERFERING; VIRUSES;

HEPATITIS C VIRUS; MAMMALIA;

EID: 84863012654     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.1119873109     Document Type: Article

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