Altering the regioselectivity of cytochrome P450 CYP102A3 of Bacillus subtilis by using a new versatile assay system

ChemBioChem

Volumn 7, Issue 2, 2006, Pages 345-350

  Lentz, Oliver ^a   Feenstra, Anton ^b   Habicher, Tilo ^c   Hauer, Bernhard ^c   Schmid, Rolf D ^a   Urlacher, Vlada B ^a  

^a UNIVERSITY OF STUTTGART   (Germany)

^b VU UNIVERSITY AMSTERDAM   (Netherlands)

^c BASF SE   (Germany)

Author keywords

Assay development; Directed evolution; Heme proteins; P450 monooxygenase; Protein engineering

Indexed keywords

CARBON; CYTOCHROME P450; CYTOCHROME P450 102A3; CYTOCHROME P450 BM3; FLAVINE ADENINE NUCLEOTIDE; FLAVINE MONONUCLEOTIDE REDUCTASE; HEME; HYBRID PROTEIN; OCTANOL; UNCLASSIFIED DRUG; UNSPECIFIC MONOOXYGENASE;

ARTICLE; BACILLUS MEGATERIUM; BACILLUS SUBTILIS; BACTERIAL GENOME; BIOCATALYST; CHEMICAL REACTION; ENZYME ASSAY; ENZYME SUBSTRATE; HIGH THROUGHPUT SCREENING; HYDROXYLATION; MUTANT; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; STEREOCHEMISTRY;

BACTERIAL PROTEINS; BIOLOGICAL ASSAY; CYTOCHROME P-450 ENZYME SYSTEM; DIRECTED MOLECULAR EVOLUTION; MIXED FUNCTION OXYGENASES; MODELS, MOLECULAR; MOLECULAR STRUCTURE; OCTANOLS; PROTEIN CONFORMATION; SUBSTRATE SPECIFICITY;

BACILLUS MEGATERIUM; BACILLUS SUBTILIS; BACTERIA (MICROORGANISMS);

EID: 32344437281     PISSN: 14394227     EISSN: None     Source Type: Journal    
DOI: 10.1002/cbic.200500266     Document Type: Article

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