Fractional factorial approach combining 4 Escherichia coli strains, 3 culture media, 3 expression temperatures and 5 N-terminal fusion tags for screening the soluble expression of recombinant proteins

Protein Expression and Purification

Volumn 84, Issue 2, 2012, Pages 204-213

  Noguère, Christophe ^a   Larsson, Anna M ^a,b   Guyot, Jean Christophe ^a   Bignon, Christophe ^a  

^a AIX MARSEILLE UNIVERSITY   (France)

^b SWEDISH UNIVERSITY OF AGRICULTURAL SCIENCES   (Sweden)

Author keywords

Design of experiment; Escherichia coli; Fractional factorial approach; Recombinant protein; Structural genomics

Indexed keywords

HIS HIS HIS HIS HIS HIS; HIS-HIS-HIS-HIS-HIS-HIS; HISTIDINE; OLIGOPEPTIDE; RECOMBINANT PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; CHEMISTRY; CULTURE MEDIUM; ESCHERICHIA COLI; GENE EXPRESSION; GENE VECTOR; GENETICS; GENOMICS; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; MOLECULAR CLONING; MOLECULAR GENETICS; SOLUBILITY; TEMPERATURE;

AMINO ACID SEQUENCE; CLONING, MOLECULAR; CULTURE MEDIA; ESCHERICHIA COLI; GENE EXPRESSION; GENETIC VECTORS; GENOMICS; HISTIDINE; MOLECULAR SEQUENCE DATA; OLIGOPEPTIDES; RECOMBINANT PROTEINS; SOLUBILITY; TEMPERATURE;

ESCHERICHIA COLI;

EID: 84862989011     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2012.05.011     Document Type: Article

References (23)

1. C. Abergel, B. Coutard, D. Byrne, S. Chenivesse, J.-B. Claude, C. Deregnaucourt, T. Fricaux, C. Gianesini-Boutreux, S. Jeudy, R. Lebrun, C. Maza, C. Notredame, O. Poirot, K. Suhre, M. Varagnol, and J.M. Claverie Structural genomics of highly conserved microbial genes of unknown function in search of new antibacterial targets J. Struct. Funct. Genomics 4 2003 141 157
2. L. Xie, D. Hall, M.A. Eiteman, and E. Altman Optimization of recombinant aminolevulinate synthase production in Escherichia coli using factorial design Appl Microbiol. Biotechnol. 63 2003 267 273
3. S.E. Swalley, J.R. Fulghum, and S.P. Chambers Screening factors affecting a response in soluble protein expression: formalized approach using design of experiments Anal Biochem. 351 2006 122 127
4. L.M.T. Paz Maldonado, V.E. Balderas Hernandez, E. Medina Rivero, A.P. Barba de la Rosa, J.L. Flores Flores, L.G. Ordonez Acevedo, and A. De Leon Rodriguez Optimization of culture conditions for a synthetic gene expression in Escherichia coli using response surface methodology: the case of human interferon beta Biomol. Eng. 24 2007 217 222
5. P.G. Blommel, K.J. Becker, P. Duvnjak, and B.G. Fox Enhanced bacterial protein expression during auto-induction obtained by alteration of lac repressor dosage and medium composition Biotechnol. Prog. 23 2007 585 598
6. R.S. Islam, D. Tisi, M.S. Levy, and G.J. Lye Framework for the rapid optimization of soluble protein expression in Escherichia coli combining microscale experiments and statistical experimental design Biotechnol. Prog. 23 2007 785 793
7. Y.P. Chuana, L.H.L. Lua, and A.P.J. Middelberg High-level expression of soluble viral structural protein in Escherichia coli J. Biotechnol. 134 2008 64 71
8. J. Dherbecourt, H. Falentin, S. Canaan, and A. Thierry A genomic search approach to identify esterases in Propionibacterium freudenreichii involved in the formation of flavour in Emmental cheese Microb. Cell Fact. 7 2008 16
9. R. Vincentelli, S. Canaan, V. Campanacci, C. Valencia, D. Maurin, F. Frassinetti, L. Scappucini-Calvo, Y. Bourne, C. Cambillau, and C. Bignon High-throughput automated refolding screening of inclusion bodies Protein Sci. 13 2004 2782 2792
10. I. Benoit, B. Coutard, R. Oubelaid, M. Asther, and C. Bignon Expression in Escherichia coli, refolding and crystallization of Aspergillus niger feruloyl esterase A using a serial factorial approach Protein Expr. Purif. 55 2007 166 174
11. B. Coutard, M. Gagnaire, A.A. Guilhon, M. Berro, S. Canaan, and C. Bignon Green fluorescent protein and factorial approach: an effective partnership for screening the soluble expression of recombinant proteins in Escherichia coli Protein Expr. Purif. 61 2008 184 190
12. R. Vincentelli, C. Bignon, A. Gruez, C. Canaan, G. Sulzenbacher, M. Tegoni, V. Campanacci, and C. Cambillau Medium-scale structural genomics: strategies for protein expression and crystallization Acc. Chem. Res. 36 2003 165 172
13. D. Esposito, and D.K. Chatterjee Enhancement of soluble protein expression through the use of fusion tags Curr. Opin. Biotechnol. 17 2006 353 358
14. F. Grueninger-Leitch, A. D'Arcy, B. D'Arcy, and C. Chene Deglycosylation of proteins for crystallization using recombinant fusion protein glycosidases Protein Sci. 5 1996 2617 2622
15. J.E. Tropea, S. Cherry, S. Nallamsetty, C. Bignon, and D.S. Waugh A generic method for the production of recombinant proteins in Escherichia coli using a dual hexahistidine-maltose-binding protein affinity tag Methods Mol. Biol. 363 2007 1 19
16. S. Alexander, and J.H. Elder Endoglycosidases from Flavobacterium meningosepticum: application to biological problems Meth. Enzymol. 179 1989 505 518
17. P. Kuhn, C. Guan, T. Cui, A.L. Tarentino, T.H. Plummer Jr, and P. Van Roey Active site and oligosaccharide recognition residues of peptide-N4-(N-acetyl-β-D-glucosaminyl)asparagine Amidase F J. Biol. Chem. 270 1995 29493 29497
18. V. Rao, T. Cui, C. Guan, and P. Van Roey Mutations of endo-β-N-acetylglucosaminidase H active site residues Asp130 and Glu132: activities and conformations Protein Sci. 8 1999 2338 2346
19. D.B. Smith, and K. Johnson Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase Gene 67 1988 31 40
20. C. Di Guan, P. Li, P.D. Riggs, and H. Inouye Vectors that facilitate the expression and purification of foreign peptides in Escherichia coli by fusion to maltose-binding protein Gene 67 1988 21 30
21. E.R. LaVallie, E.A. DiBlasio, S. Kovacic, K.L. Grant, P.F. Schendel, and J.M. McCoy A Thioredoxin gene fusion expression system that circumvents inclusion body formation in the E. coli cytoplasm Bio/Technology 11 1993 187 193
22. G.D. Davis, C. Elisee, D.M. Newham, and R.G. Harrison New fusion protein systems designed to give soluble expression in Escherichia coli Biotechnol. Bioeng. 65 1999 382 388
23. L. Niiranen, S. Espelid, C.R. Karlsen, M. Mustonen, S.M. Paulsen, P. Heikinheimo, and N.P. Willassen Comparative expression study to increase the solubility of cold adapted Vibrio proteins in Escherichia coli Protein Expr. Purif. 52 2007 210 218