Green fluorescent protein and factorial approach: An effective partnership for screening the soluble expression of recombinant proteins in Escherichia coli

Protein Expression and Purification

Volumn 61, Issue 2, 2008, Pages 184-190

  Coutard, Bruno ^a   Gagnaire, Mathieu ^a   Guilhon, Aude Agnès ^a   Berro, Marine ^a   Canaan, Stéphane ^b   Bignon, Christophe ^a  

^a AIX MARSEILLE UNIVERSITY   (France)

^b Centre National de la Recherche Scientifique   (France)

Author keywords

Design of experiment; E. coli; Factorial approach; Green fluorescence protein; Protein expression; Protein solubility; Recombinant protein

Indexed keywords

GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN;

ARTICLE; BIOSYNTHESIS; CHEMISTRY; ESCHERICHIA COLI; GENE EXPRESSION; GENE TRANSFER; GENETICS; METABOLISM; MOLECULAR CLONING; REPORTER GENE; SOLUBILITY; WESTERN BLOTTING;

BLOTTING, WESTERN; CLONING, MOLECULAR; ESCHERICHIA COLI; GENE EXPRESSION; GENE TRANSFER TECHNIQUES; GENES, REPORTER; GREEN FLUORESCENT PROTEINS; RECOMBINANT FUSION PROTEINS; SOLUBILITY;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 50049102681     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2008.05.014     Document Type: Article

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