The Karyopherin Sal3 is Required for Nuclear Import of the Core RNA Interference Pathway Protein Rdp1

Traffic

Volumn 13, Issue 4, 2012, Pages 520-531

  Park, Jungsook ^a   Freitag, Silja I ^b   Young, Paul G ^b   Hobman, Tom C ^a,c  

^a UNIVERSITY OF ALBERTA   (Canada)

^b QUEEN S UNIVERSITY   (Canada)

^c UNIVERSITY OF ALBERTA   (Canada)

Author keywords

Fission yeast; Karyopherin; Nuclear transport; Rdp1; RNA interference; RNA dependent RNA polymerase; Transcriptional gene silencing

Indexed keywords

KARYOPHERIN BETA; KARYOPHERIN SAL3; RNA DIRECTED RNA POLYMERASE; RNA DIRECTED RNA POLYMERASE 1; TIABENDAZOLE; UNCLASSIFIED DRUG;

ARTICLE; CONTROLLED STUDY; CYTOPLASM; EUKARYOTE; NONHUMAN; NUCLEAR IMPORT; POSTTRANSCRIPTIONAL GENE SILENCING; PRIORITY JOURNAL; PROTEIN DEPLETION; PROTEIN TRANSPORT; RNA INTERFERENCE; SCHIZOSACCHAROMYCES POMBE;

ACTIVE TRANSPORT, CELL NUCLEUS; ADENOSINE TRIPHOSPHATASES; KARYOPHERINS; RNA, SMALL INTERFERING; SACCHAROMYCES; SCHIZOSACCHAROMYCES POMBE PROTEINS; SIGNAL TRANSDUCTION;

EID: 84862825917     PISSN: 13989219     EISSN: 16000854     Source Type: Journal    
DOI: 10.1111/j.1600-0854.2012.01333.x     Document Type: Article

References (37)

1. Hannon GJ. RNA interference. Nature 2002;418:244-251.
2. Sigova A, Rhind N, Zamore PD. A single Argonaute protein mediates both transcriptional and posttranscriptional silencing in Schizosaccharomyces pombe. Genes Dev 2004;18:2359-2367.
3. Volpe TA, Kidner C, Hall IM, Teng G, Grewal SI, Martienssen RA. Regulation of heterochromatic silencing and histone H3 lysine-9 methylation by RNAi. Science 2002;297:1833-1837.
4. Hall IM, Shankaranarayana GD, Noma K, Ayoub N, Cohen A, Grewal SI. Establishment and maintenance of a heterochromatin domain. Science 2002;297:2232-2237.
5. Provost P, Dishart D, Doucet J, Frendewey D, Samuelsson B, Radmark O. Ribonuclease activity and RNA binding of recombinant human Dicer. EMBO J 2002;21:5864-5874.
6. Carmichael JB, Stoica C, Parker H, McCaffery JM, Simmonds AJ, Hobman TC. RNA interference effector proteins localize to mobile cytoplasmic puncta in Schizosaccharomyces pombe. Traffic 2006;7:1032-1044.
7. Motamedi MR, Verdel A, Colmenares SU, Gerber SA, Gygi SP, Moazed D. Two RNAi complexes, RITS and RDRC, physically interact and localize to noncoding centromeric RNAs. Cell 2004;119:789-802.
8. Verdel A, Jia S, Gerber S, Sugiyama T, Gygi S, Grewal SI, Moazed D. RNAi-mediated targeting of heterochromatin by the RITS complex. Science 2004;303:672-676.
9. Sugiyama T, Cam H, Verdel A, Moazed D, Grewal SI. RNA-dependent RNA polymerase is an essential component of a self-enforcing loop coupling heterochromatin assembly to siRNA production. Proc Natl Acad Sci U S A 2005;102:152-157.
10. Terry LJ, Shows EB, Wente SR. Crossing the nuclear envelope: hierarchical regulation of nucleocytoplasmic transport. Science 2007;318:1412-1416.
11. Wozniak RW, Rout MP, Aitchison JD. Karyopherins and kissing cousins. Trends Cell Biol 1998;8:184-188.
12. Weinmann L, Hock J, Ivacevic T, Ohrt T, Mutze J, Schwille P, Kremmer E, Benes V, Urlaub H, Meister G. Importin 8 is a gene silencing factor that targets Argonaute proteins to distinct mRNAs. Cell 2009;136:496-507.
13. Guang S, Bochner AF, Pavelec DM, Burkhart KB, Harding S, Lachowiec J, Kennedy S. An Argonaute transports siRNAs from the cytoplasm to the nucleus. Science 2008;321:537-541.
14. Djupedal I, Ekwall K. Epigenetics: heterochromatin meets RNAi. Cell Res 2009;19:282-295.
15. Chen XQ, Du X, Liu J, Balasubramanian MK, Balasundaram D. Identification of genes encoding putative nucleoporins and transport factors in the fission yeast Schizosaccharomyces pombe: a deletion analysis. Yeast 2004;21:495-509.
16. Chua G, Lingner C, Frazer C, Young PG. The sal3(+) gene encodes an importin-beta implicated in the nuclear import of Cdc25 in Schizosaccharomyces pombe. Genetics 2002;162:689-703.
17. Stoica C, Carmichael JB, Parker H, Pare J, Hobman TC. Interactions between the RNA interference effector protein Ago1 and 14-3-3 proteins: consequences for cell cycle progression. J Biol Chem 2006;281:37646-37651.
18. Stoica C, Park J, Pare JM, Willows S, Hobman TC. The Kinesin motor protein Cut7 regulates biogenesis and function of Ago1-complexes. Traffic 2010;11:25-36.
19. Volpe T, Schramke V, Hamilton GL, White SA, Teng G, Martienssen RA, Allshire RC. RNA interference is required for normal centromere function in fission yeast. Chromosome Res 2003;11:137-146.
20. Kalderon D, Roberts BL, Richardson WD, Smith AE. A short amino acid sequence able to specify nuclear location. Cell 1984;39:499-509.
21. Pare JM, Tahbaz N, Lopez-Orozco J, LaPointe P, Lasko P, Hobman TC. Hsp90 regulates the function of argonaute 2 and its recruitment to stress granules and P-bodies. Mol Biol Cell 2009;20:3273-3284.
22. Stoica C, Park J, Pare JM, Willows S, Hobman TC. The Kinesin motor protein Cut7 regulates biogenesis and function of Ago1-complexes. Traffic 11:25-36.
23. Loschi M, Leishman CC, Berardone N, Boccaccio GL. Dynein and kinesin regulate stress-granule and P-body dynamics. J Cell Sci 2009;122:3973-3982.
24. Weinmann L, Hock J, Ivacevic T, Ohrt T, Mutze J, Schwille P, Kremmer E, Benes V, Urlaub H, Meister G. Importin 8 is a gene silencing factor that targets argonaute proteins to distinct mRNAs. Cell 2009;136:496-507.
25. Irvine DV, Zaratiegui M, Tolia NH, Goto DB, Chitwood DH, Vaughn MW, Joshua-Tor L, Martienssen RA. Argonaute slicing is required for heterochromatic silencing and spreading. Science 2006;313:1134-1137.
26. Djupedal I, Kos-Braun IC, Mosher RA, Soderholm N, Simmer F, Hardcastle TJ, Fender A, Heidrich N, Kagansky A, Bayne E, Wagner EG, Baulcombe DC, Allshire RA, Ekwall K. Analysis of small RNA in fission yeast; centromeric siRNAs are potentially generated through a structured RNA. EMBO J 2009;28:3832-3844.
27. Halic M, Moazed D. Dicer-independent primal RNAs trigger RNAi and heterochromatin formation. Cell 2010;140:504-516.
28. Noma KI, Sugiyama T, Cam H, Verdel A, Zofall M, Jia S, Moazed D, Grewal SI. RITS acts in cis to promote RNA interference-mediated transcriptional and post-transcriptional silencing. Nat Genet 2004;36:1174-1180.
29. Simmer F, Buscaino A, Kos-Braun IC, Kagansky A, Boukaba A, Urano T, Kerr AR, Allshire RC. Hairpin RNA induces secondary small interfering RNA synthesis and silencing in trans in fission yeast. EMBO Rep 2010;11:112-118.
30. Lipardi C, Paterson BM. Identification of an RNA-dependent RNA polymerase in Drosophila establishes a common theme in RNA silencing. Fly (Austin) 2010;4:30-35.
31. Lipardi C, Paterson BM. Identification of an RNA-dependent RNA polymerase in Drosophila involved in RNAi and transposon suppression. Proc Natl Acad Sci U S A 2009;106:15645-15650.
32. Alfa C, Fantes P, Hyams J, McLeod M, Warbrick E. Experiments with Fission Yeast: A Laboratory Course Manual. Plainview: Cold Spring Harbor Laboratory Press; 1993.
33. Maundrell K. nmt1 of fission yeast. A highly transcribed gene completely repressed by thiamine. J Biol Chem 1990;265:10857-10864.
34. Xu HP, Rajavashisth T, Grewal N, Jung V, Riggs M, Rodgers L, Wigler M. A gene encoding a protein with seven zinc finger domains acts on the sexual differentiation pathways of Schizosaccharomyces pombe. Mol Biol Cell 1992;3:721-734.
35. Pidoux AL, Uzawa S, Perry PE, Cande WZ, Allshire RC. Live analysis of lagging chromosomes during anaphase and their effect on spindle elongation rate in fission yeast. J Cell Sci 2000;113:4177-4191.
36. Moreno S, Klar A, Nurse P. Molecular genetic analysis of fission yeast Schizosaccharomyces pombe. Methods Enzymol 1991;194:795-823.
37. Sambrook JF, Fritsch EF, Maniatis T. 2nd ed. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor: Cold Spring Harbor Laboratory Press; 1989.