A reversed-phase capillary ultra-performance liquid chromatography-mass spectrometry (UPLC-MS) method for comprehensive top-down/bottom-up lipid profiling

Analytical and Bioanalytical Chemistry

Volumn 402, Issue 9, 2012, Pages 2923-2933

  Gao, Xiaoli ^a   Zhang, Qibin ^a   Meng, Da ^a   Isaac, Giorgis ^a   Zhao, Rui ^b   Fillmore, Thomas L ^b   Chu, Rosey K ^b   Zhou, Jianying ^a   Tang, Keqi ^a   Hu, Zeping ^a   Moore, Ronald J ^a   Smith, Richard D ^a   Katze, Michael G ^c,d   Metz, Thomas O ^a  

^a PACIFIC NORTHWEST NATIONAL LABORATORY   (United States)

^b PACIFIC NORTHWEST NATIONAL LABORATORY   (United States)

^c UNIVERSITY OF WASHINGTON SCHOOL OF MEDICINE   (United States)

^d UNIVERSITY OF WASHINGTON   (United States)

Author keywords

Electrospray ionization (ESI); Tandem mass spectrometry (MS MS); Top down bottom up lipid profiling; Ultra performance liquid chromatography (UPLC)

Indexed keywords

COMPLEX SAMPLES; CRITICAL PARTS; HUMAN CELL LINES; HUMAN SKIN; LIMIT OF QUANTIFICATIONS; LIPIDOMES; LIPIDOMICS; LIVING SYSTEMS; MEASUREMENT SENSITIVITY; METABOLOMICS; ORDERS OF MAGNITUDE; PEAK CAPACITY; RAT PLASMA; RELATIVE STANDARD DEVIATIONS; REPRODUCIBILITIES; RETENTION TIME; REVERSED PHASE; STATIONARY PHASIS; TANDEM MASS SPECTROMETRY; TOPDOWN; ULTRA-PERFORMANCE LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY; ULTRAPERFORMANCE LIQUID CHROMATOGRAPHY;

CELL CULTURE; ELECTROSPRAY IONIZATION; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; TISSUE;

LIPIDS;

LIPID;

ANIMAL; ARTICLE; CELL LINE; EVALUATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; METHODOLOGY; RAT; REVERSED PHASE LIQUID CHROMATOGRAPHY;

ANIMALS; CELL LINE; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CHROMATOGRAPHY, REVERSE-PHASE; LIPIDS; MASS SPECTROMETRY; RATS;

RATTUS;

EID: 84862778693     PISSN: 16182642     EISSN: 16182650     Source Type: Journal    
DOI: 10.1007/s00216-012-5773-5     Document Type: Article

References (66)

1. G van Meer DR Voelker GW Feigenson 2008 Membrane lipids: where they are and how they behave Nat Rev Mol Cell Biol 9 2 112 124 10.1038/nrm2330 10.1038/nrm2330 (Pubitemid 351158912)
2. A Astrup J Dyerberg M Selleck S Stender 2008 Nutrition transition and its relationship to the development of obesity and related chronic diseases Obes Rev 9 Suppl 1 48 52 10.1111/j.1467-789X.2007.00438.x 10.1111/j.1467-789X.2007. 00438.x (Pubitemid 351643190)
3. GL Russo 2009 Dietary n-6 and n-3 polyunsaturated fatty acids: from biochemistry to clinical implications in cardiovascular prevention Biochem Pharmacol 77 6 937 946 10.1016/j.bcp. 2008.10.020 10.1016/j.bcp.2008.10.020 1:CAS:528:DC%2BD1MXisFOgsrg%3D
4. DL Brasaemle 2007 Thematic review series: adipocyte biology. The perilipin family of structural lipid droplet proteins: stabilization of lipid droplets and control of lipolysis J Lipid Res 48 12 2547 2559 10.1194/jlr.R700014-JLR200 10.1194/jlr.R700014-JLR200 1:CAS:528: DC%2BD2sXhsValtL7E (Pubitemid 350272839)
5. MR Wenk 2005 The emerging field of lipidomics Nat Rev Drug Discov 4 7 594 610 10.1038/nrd1776 10.1038/nrd1776 1:CAS:528:DC%2BD2MXmsFGjtbs%3D (Pubitemid 40975712)
6. M Oresic S Simell M Sysi-Aho K Nanto-Salonen T Seppanen-Laakso V Parikka M Katajamaa A Hekkala I Mattila P Keskinen L Yetukuri A Reinikainen J Lahde T Suortti J Hakalax T Simell H Hyoty R Veijola J Ilonen R Lahesmaa M Knip O Simell 2008 Dysregulation of lipid and amino acid metabolism precedes islet autoimmunity in children who later progress to type 1 diabetes J Exp Med 205 13 2975 2984 10.1084/jem.20081800 10.1084/jem.20081800 1:CAS:528:DC%2BD1MXosFCg
7. CM Sorensen J Ding Q Zhang T Alquier R Zhao PW Mueller RD Smith TO Metz 2010 Perturbations in the lipid profile of individuals with newly diagnosed type 1 diabetes mellitus: lipidomics analysis of a Diabetes Antibody Standardization Program sample subset Clin Biochem 43 12 948 956 10.1016/j.clinbiochem.2010.04. 075 10.1016/j.clinbiochem.2010.04.075 1:CAS:528:DC%2BC3cXos1Gnurw%3D
8. RW Gross X Han 2007 Lipidomics in diabetes and the metabolic syndrome Methods Enzymol 433 73 90 10.1016/S0076-6879(07)33004-8 10.1016/S0076-6879(07) 33004-8 1:CAS:528:DC%2BD1cXhsVOqsbw%3D
9. X Han 2010 Multi-dimensional mass spectrometry-based shotgun lipidomics and the altered lipids at the mild cognitive impairment stage of Alzheimer's disease Biochim Biophys Acta 1801 8 774 783 10.1016/j.bbalip. 2010.01.010 1:CAS:528:DC%2BC3cXntlagsrw%3D
10. L Zhao SD Spassieva TJ Jucius LD Shultz HE Shick WB Macklin YA Hannun LM Obeid SL Ackerman 2011 A deficiency of ceramide biosynthesis causes cerebellar Purkinje cell neurodegeneration and lipofuscin accumulation PLoS Genet 7 5 e1002063 10.1371/journal.pgen.1002063 10.1371/journal.pgen.1002063 1:CAS:528:DC%2BC3MXms1KntLk%3D
11. RG Cutler J Kelly K Storie WA Pedersen A Tammara K Hatanpaa JC Troncoso MP Mattson 2004 Involvement of oxidative stress-induced abnormalities in ceramide and cholesterol metabolism in brain aging and Alzheimer's disease Proc Natl Acad Sci USA 101 7 2070 2075 10.1073/pnas.0305799101 10.1073/pnas. 0305799101 1:CAS:528:DC%2BD2cXhs1Srsrw%3D (Pubitemid 38229016)
12. AZ Fernandis MR Wenk 2009 Lipid-based biomarkers for cancer J Chromatogr B Analyt Technol Biomed Life Sci 877 26 2830 2835 10.1016/j.jchromb.2009.06.015 10.1016/j.jchromb.2009.06.015 1:CAS:528:DC%2BD1MXps1WhtLs%3D
13. EO Aboagye ZM Bhujwalla 1999 Malignant transformation alters membrane choline phospholipid metabolism of human mammary epithelial cells Cancer Res 59 1 80 84 1:CAS:528:DyaK1MXmsFOgtg%3D%3D (Pubitemid 29062961)
14. DL Diamond AJ Syder JM Jacobs CM Sorensen KA Walters SC Proll JE McDermott MA Gritsenko Q Zhang R Zhao TO Metz DG Camp 2nd KM Waters RD Smith CM Rice MG Katze 2010 Temporal proteome and lipidome profiles reveal hepatitis C virus-associated reprogramming of hepatocellular metabolism and bioenergetics PLoS Pathog 6 1 e1000719 10.1371/journal.ppat.1000719 10.1371/journal.ppat. 1000719
15. M Jain CJ Petzold MW Schelle MD Leavell JD Mougous CR Bertozzi JA Leary JS Cox 2007 Lipidomics reveals control of Mycobacterium tuberculosis virulence lipids via metabolic coupling Proc Natl Acad Sci USA 104 12 5133 5138 10.1073/pnas.0610634104 10.1073/pnas.0610634104 1:CAS:528:DC%2BD2sXjvFCgsrs%3D (Pubitemid 47186188)
16. MR Wenk 2006 Lipidomics of host-pathogen interactions FEBS Lett 580 23 5541 5551 10.1016/j.febslet.2006.07.007 10.1016/j.febslet.2006.07.007 1:CAS:528:DC%2BD28XhtVajsr%2FJ (Pubitemid 44465899)
17. E Fahy S Subramaniam HA Brown CK Glass AH Merrill Jr RC Murphy CR Raetz DW Russell Y Seyama W Shaw T Shimizu F Spener G van Meer MS VanNieuwenhze SH White JL Witztum EA Dennis 2005 A comprehensive classification system for lipids J Lipid Res 46 5 839 861 10.1194/jlr.E400004-JLR200 10.1194/jlr.E400004-JLR200 1:CAS:528:DC%2BD2MXktFaqtLY%3D
18. E Fahy S Subramaniam RC Murphy M Nishijima CR Raetz T Shimizu F Spener G van Meer MJ Wakelam EA Dennis 2009 Update of the LIPID MAPS comprehensive classification system for lipids J Lipid Res 50 Suppl S9 14 10.1194/jlr.R800095- JLR200 10.1194/jlr.R800095-JLR200
19. R Harkewicz EA Dennis 2011 Applications of mass spectrometry to lipids and membranes Annu Rev Biochem 80 301 325 10.1146/annurev-biochem-060409-092612 10.1146/annurev-biochem-060409-092612 1:CAS:528:DC%2BC3MXptVCnsL8%3D
20. X Han RW Gross 2005 Shotgun lipidomics: electrospray ionization mass spectrometric analysis and quantitation of cellular lipidomes directly from crude extracts of biological samples Mass Spectrom Rev 24 3 367 412 10.1002/mas.20023 10.1002/mas.20023 1:CAS:528:DC%2BD2MXjvFOmu78%3D (Pubitemid 40665798)
21. KL Duffin JD Henion JJ Shieh 1991 Electrospray and tandem mass spectrometric characterization of acylglycerol mixtures that are dissolved in nonpolar solvents Anal Chem 63 17 1781 1788 10.1021/ac00017a023 1:STN:280:DyaK387mtFegsA%3D%3D
22. EA Dennis 2009 Lipidomics joins the omics evolution Proc Natl Acad Sci USA 106 7 2089 2090 10.1073/pnas.0812636106 10.1073/pnas.0812636106 1:CAS:528:DC%2BD1MXitlaqsLc%3D
23. TW Mitchell H Pham MC Thomas SJ Blanksby 2009 Identification of double bond position in lipids: from GC to OzID J Chromatogr B Analyt Technol Biomed Life Sci 877 26 2722 2735 10.1016/j.jchromb.2009.01.017 10.1016/j.jchromb.2009. 01.017 1:CAS:528:DC%2BD1MXps1Whtrg%3D
24. M Li Z Zhou H Nie Y Bai H Liu 2011 Recent advances of chromatography and mass spectrometry in lipidomics Anal Bioanal Chem 399 1 243 249 10.1007/s00216-010-4327-y 10.1007/s00216-010-4327-y 1:CAS:528:DC%2BC3cXhtlygtLfJ
25. PM Hutchins RM Barkley RC Murphy 2008 Separation of cellular nonpolar neutral lipids by normal-phase chromatography and analysis by electrospray ionization mass spectrometry J Lipid Res 49 4 804 813 10.1194/jlr.M700521-JLR200 10.1194/jlr.M700521-JLR200 1:CAS:528:DC%2BD1cXjvFegsLo%3D
26. H Nie R Liu Y Yang Y Bai Y Guan D Qian T Wang H Liu 2010 Lipid profiling of rat peritoneal surface layers by online normal- and reversed-phase 2D LC QToF-MS J Lipid Res 51 9 2833 2844 10.1194/jlr.D007567 10.1194/jlr.D007567 1:CAS:528:DC%2BC3cXhtVOltrzP
27. J Ding CM Sorensen N Jaitly H Jiang DJ Orton ME Monroe RJ Moore RD Smith TO Metz 2008 Application of the accurate mass and time tag approach in studies of the human blood lipidome J Chromatogr B Analyt Technol Biomed Life Sci 871 2 243 252 10.1016/j.jchromb.2008.04.040 10.1016/j.jchromb.2008.04.040 1:CAS:528:DC%2BD1cXps12itrg%3D
28. K Sandra S Pereira Ados G Vanhoenacker F David P Sandra 2010 Comprehensive blood plasma lipidomics by liquid chromatography/quadrupole time-of-flight mass spectrometry J Chromatogr A 1217 25 4087 4099 10.1016/j.chroma.2010.02.039 10.1016/j.chroma.2010.02.039 1:CAS:528: DC%2BC3cXmvVWju74%3D
29. TO Metz Q Zhang JS Page Y Shen SJ Callister JM Jacobs RD Smith 2007 The future of liquid chromatography-mass spectrometry (LC-MS) in metabolic profiling and metabolomic studies for biomarker discovery Biomark Med 1 1 159 185 10.2217/17520363.1.1.159 10.2217/17520363.1.1.159 1:CAS:528:DC%2BD2sXosVartrg%3D
30. H Nygren T Seppanen-Laakso S Castillo T Hyotylainen M Oresic 2011 Liquid chromatography-mass spectrometry (LC-MS)-based lipidomics for studies of body fluids and tissues Methods Mol Biol 708 247 257 10.1007/978-1-61737-985-7-15 10.1007/978-1-61737-985-7-15 1:CAS:528:DC%2BC3MXmsVSgtbo%3D
31. M Masoodi M Eiden A Koulman D Spaner DA Volmer 2010 Comprehensive lipidomics analysis of bioactive lipids in complex regulatory networks Anal Chem 82 19 8176 8185 10.1021/ac1015563 10.1021/ac1015563 1:CAS:528: DC%2BC3cXhtFartrzE
32. PD Rainville CL Stumpf JP Shockcor RS Plumb JK Nicholson 2007 Novel application of reversed-phase UPLC-oaTOF-MS for lipid analysis in complex biological mixtures: a new tool for lipidomics J Proteome Res 6 2 552 558 10.1021/pr060611b 10.1021/pr060611b 1:CAS:528:DC%2BD28XhtlCrs7rI (Pubitemid 46340159)
33. Shockcor J, Crowe H, Yu K, Shion H (2010) Analysis of intact lipids from biologics matrices by UPLC/high definition MS. Application Note. Waters Corporation, Milford, MA
34. Schuhmann K, Herzog R, Schwudke D, Metelmann-Strupat W, Bornstein SR, Shevchenko A (2011) Bottom-up shotgun lipidomics by higher energy collisional dissociation (HCD) on LTQ orbitrap mass spectrometers. Anal Chem. doi: 10.1021/ac102505f
35. BK Matuszewski ML Constanzer CM Chavez-Eng 2003 Strategies for the assessment of matrix effect in quantitative bioanalytical methods based on HPLC-MS/MS Anal Chem 75 13 3019 3030 10.1021/ac020361s 1:CAS:528: DC%2BD3sXltV2jsL8%3D (Pubitemid 36830428)
36. RT Kelly JS Page Q Luo RJ Moore DJ Orton K Tang RD Smith 2006 Chemically etched open tubular and monolithic emitters for nanoelectrospray ionization mass spectrometry Anal Chem 78 22 7796 7801 10.1021/ac061133r 10.1021/ac061133r 1:CAS:528:DC%2BD28XhtVyntLbO (Pubitemid 44788724)
37. GR Kiebel KJ Auberry N Jaitly DA Clark ME Monroe ES Peterson N Tolic GA Anderson RD Smith 2006 PRISM: a data management system for high-throughput proteomics Proteomics 6 6 1783 1790 10.1002/pmic.200500500 10.1002/pmic. 200500500 1:CAS:528:DC%2BD28XjtlKmtr4%3D
38. N Jaitly A Mayampurath K Littlefield JN Adkins GA Anderson RD Smith 2009 Decon2LS: an open-source software package for automated processing and visualization of high resolution mass spectrometry data BMC Bioinformatics 10 87 10.1186/1471-2105-10-87 10.1186/1471-2105-10-87
39. ME Monroe N Tolic N Jaitly JL Shaw JN Adkins RD Smith 2007 VIPER: an advanced software package to support high-throughput LC-MS peptide identification Bioinformatics 23 15 2021 2023 10.1093/bioinformatics/btm281 10.1093/bioinformatics/btm281 1:CAS:528:DC%2BD2sXpt1Cru7g%3D (Pubitemid 47299846)
40. N Jaitly ME Monroe VA Petyuk TR Clauss JN Adkins RD Smith 2006 Robust algorithm for alignment of liquid chromatography-mass spectrometry analyses in an accurate mass and time tag data analysis pipeline Anal Chem 78 21 7397 7409 10.1021/ac052197p 10.1021/ac052197p 1:CAS:528:DC%2BD28XhtVakurnE (Pubitemid 44707644)
41. O Quehenberger AM Armando AH Brown SB Milne DS Myers AH Merrill S Bandyopadhyay KN Jones S Kelly RL Shaner CM Sullards E Wang RC Murphy RM Barkley TJ Leiker CR Raetz Z Guan GM Laird DA Six DW Russell JG McDonald S Subramaniam E Fahy EA Dennis 2010 Lipidomics reveals a remarkable diversity of lipids in human plasma J Lipid Res 51 11 3299 3305 10.1194/jlr.M009449 10.1194/jlr.M009449 1:CAS:528:DC%2BC3cXhsVWgt7vN
42. NL Anderson NG Anderson 2002 The human plasma proteome: history, character, and diagnostic prospects Mol Cell Proteomics 1 11 845 867 10.1074/mcp.R200007-MCP200 1:CAS:528:DC%2BD3sXht1Ciug%3D%3D
43. A Schmidt M Karas T Dulcks 2003 Effect of different solution flow rates on analyte ion signals in nano-ESI MS, or: when does ESI turn into nano-ESI? J Am Soc Mass Spectrom 14 5 492 500 10.1016/S1044-0305(03)00128-4 10.1016/S1044-0305(03)00128-4 1:CAS:528:DC%2BD3sXjs1ahtbg%3D (Pubitemid 36871630)
44. RD Smith KQ Tang JS Page 2004 Charge competition and the linear dynamic range of detection in electrospray ionization mass spectrometry J Am Soc Mass Spectrom 15 10 1416 1423 10.1016/j.jasms.2004.04.034 10.1016/j.jasms.2004.04.034 (Pubitemid 39311692)
45. Y Shen JM Jacobs DG Camp 2nd R Fang RJ Moore RD Smith W Xiao RW Davis RG Tompkins 2004 Ultra-high-efficiency strong cation exchange LC/RPLC/MS/MS for high dynamic range characterization of the human plasma proteome Anal Chem 76 4 1134 1144 10.1021/ac034869m 10.1021/ac034869m 1:CAS:528:DC%2BD2cXksFOjsA%3D%3D (Pubitemid 38235564)
46. MS Wilm M Mann 1994 Electrospray and Taylor-Cone Theory, Doles Beam of macromolecules at last Int J Mass Spectrom 136 2-3 167 180 10.1016/0168-1176(94) 04024-9 1:CAS:528:DyaK2MXhslOgs70%3D
47. JF Delamora IG Loscertales 1994 The current emitted by highly conducting Taylor Cones J Fluid Mech 260 155 184 10.1017/S0022112094003472 (Pubitemid 279257)
48. R Plumb J Castro-Perez J Granger I Beattie K Joncour A Wright 2004 Ultra-performance liquid chromatography coupled to quadrupole-orthogonal time-of-flight mass spectrometry Rapid Commun Mass Spectrom 18 19 2331 2337 10.1002/rcm.1627 10.1002/rcm.1627 1:CAS:528:DC%2BD2cXot1Cmsbg%3D (Pubitemid 39297371)
49. JJ Vandeemter FJ Zuiderweg A Klinkenberg 1956 Longitudinal diffusion and resistance to mass transfer as causes of nonideality in chromatography Chem Eng Sci 5 6 271 289 10.1016/0009-2509(56)80003-1 1:CAS:528:DyaG2sXktVWi
50. JM Castro-Perez J Kamphorst J DeGroot F Lafeber J Goshawk K Yu JP Shockcor RJ Vreeken T Hankemeier 2010 Comprehensive LC-MS E lipidomic analysis using a shotgun approach and its application to biomarker detection and identification in osteoarthritis patients J Proteome Res 9 5 2377 2389 10.1021/pr901094j 10.1021/pr901094j 1:CAS:528:DC%2BC3cXktVWltLc%3D
51. Y Shen R Zhao SJ Berger GA Anderson N Rodriguez RD Smith 2002 High-efficiency nanoscale liquid chromatography coupled on-line with mass spectrometry using nanoelectrospray ionization for proteomics Anal Chem 74 16 4235 4249 10.1021/ac0202280 1:CAS:528:DC%2BD38XkvF2qt70%3D (Pubitemid 34912927)
52. Giddings JC (1991) United separation science. Wiley, New York
53. Y Shen R Zhang RJ Moore J Kim TO Metz KK Hixson R Zhao EA Livesay HR Udseth RD Smith 2005 Automated 20 kpsi RPLC-MS and MS/MS with chromatographic peak capacities of 1000-1500 and capabilities in proteomics and metabolomics Anal Chem 77 10 3090 3100 10.1021/ac0483062 10.1021/ac0483062 1:CAS:528:DC%2BD2MXivFWku78%3D (Pubitemid 40695464)
54. J Graessler D Schwudke PE Schwarz R Herzog A Shevchenko SR Bornstein 2009 Top-down lipidomics reveals ether lipid deficiency in blood plasma of hypertensive patients PLoS One 4 7 e6261 10.1371/journal.pone.0006261 10.1371/journal.pone.0006261
55. D Schwudke JT Hannich V Surendranath V Grimard T Moehring L Burton T Kurzchalia A Shevchenko 2007 Top-down lipidomic screens by multivariate analysis of high-resolution survey mass spectra Anal Chem 79 11 4083 4093 10.1021/ac062455y 10.1021/ac062455y 1:CAS:528:DC%2BD2sXkvValsLY%3D (Pubitemid 46903157)
56. M Pulfer RC Murphy 2003 Electrospray mass spectrometry of phospholipids Mass Spectrom Rev 22 5 332 364 10.1002/mas.10061 10.1002/mas.10061 1:CAS:528:DC%2BD3sXovValtr0%3D
57. K Yang H Cheng RW Gross X Han 2009 Automated lipid identification and quantification by multidimensional mass spectrometry-based shotgun lipidomics Anal Chem 81 11 4356 4368 10.1021/ac900241u 10.1021/ac900241u 1:CAS:528:DC%2BD1MXlt1Knu7w%3D
58. JS Fang MJ Barcelona 1998 Structural determination and quantitative analysis of bacterial phospholipids using liquid chromatography electrospray ionization mass spectrometry J Microbiol Methods 33 1 23 35 10.1016/S0167- 7012(98)00037-2 1:CAS:528:DyaK1cXktFKmsrk%3D (Pubitemid 28288950)
59. JL Bushee UA Argikar 2011 An experimental approach to enhance precursor ion fragmentation for metabolite identification studies: application of dual collision cells in an orbital trap Rapid Commun Mass Spectrom 25 10 1356 1362 10.1002/rcm.4996 10.1002/rcm.4996 1:CAS:528:DC%2BC3MXkvF2isrg%3D
60. O Quehenberger A Armando D Dumlao DL Stephens EA Dennis 2008 Lipidomics analysis of essential fatty acids in macrophages Prostaglandins Leukot Essent Fatty Acids 79 3-5 123 129 10.1016/j.plefa.2008.09.021 10.1016/j.plefa.2008.09. 021 1:CAS:528:DC%2BD1cXhsVGhtLbK
61. A Thomas J Deglon S Lenglet F Mach P Mangin JL Wolfender S Steffens C Staub 2010 High-throughput phospholipidic fingerprinting by online desorption of dried spots and quadrupole-linear ion trap mass spectrometry: evaluation of atherosclerosis biomarkers in mouse plasma Anal Chem 82 15 6687 6694 10.1021/ac101421b 10.1021/ac101421b 1:CAS:528:DC%2BC3cXovFehurg%3D
62. EJ Ahn H Kim BC Chung G Kong MH Moon 2008 Quantitative profiling of phosphatidylcholine and phosphatidylethanolamine in a steatosis/fibrosis model of rat liver by nanoflow liquid chromatography/tandem mass spectrometry J Chromatogr A 1194 1 96 102 10.1016/j.chroma.2008.04.031 10.1016/j.chroma.2008. 04.031 1:CAS:528:DC%2BD1cXms1Whurk%3D
63. H Kim E Ahn MH Moon 2008 Profiling of human urinary phospholipids by nanoflow liquid chromatography/tandem mass spectrometry Analyst 133 12 1656 1663 10.1039/b804715d 10.1039/b804715d 1:CAS:528:DC%2BD1cXhsVWmt7%2FF
64. H Kim HK Min G Kong MH Moon 2009 Quantitative analysis of phosphatidylcholines and phosphatidylethanolamines in urine of patients with breast cancer by nanoflow liquid chromatography/tandem mass spectrometry Anal Bioanal Chem 393 6-7 1649 1656 10.1007/s00216-009-2621-3 10.1007/s00216-009- 2621-3 1:CAS:528:DC%2BD1MXhsFWisLs%3D
65. JY Lee HK Min MH Moon 2011 Simultaneous profiling of lysophospholipids and phospholipids from human plasma by nanoflow liquid chromatography-tandem mass spectrometry Anal Bioanal Chem 400 9 2953 2961 10.1007/s00216-011-4958-7 10.1007/s00216-011-4958-7 1:CAS:528:DC%2BC3MXkslCmt7s%3D
66. R Taguchi T Houjou H Nakanishi T Yamazaki M Ishida M Imagawa T Shimizu 2005 Focused lipidomics by tandem mass spectrometry J Chromatogr B Analyt Technol Biomed Life Sci 823 1 26 36 10.1016/j.jchromb.2005.06.005 10.1016/j.jchromb.2005.06.005 1:CAS:528:DC%2BD2MXmvVWmuro%3D (Pubitemid 41073986)