Efficient recovery of whole blood RNA - a comparison of commercial RNA extraction protocols for high-throughput applications in wildlife species

BMC Biotechnology

Volumn 12, Issue , 2012, Pages

  Schwochow, Doreen ^a,b   Serieys, Laurel E K ^a   Wayne, Robert K ^a   Thalmann, Olaf ^a,c  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b SWEDISH UNIVERSITY OF AGRICULTURAL SCIENCES   (Sweden)

^c UNIVERSITY OF TURKU   (Finland)

Author keywords

Dna contamination; Globin transcripts; Rna extraction; Rna preservation; Transcriptome sequencing; Whole blood

Indexed keywords

CANDIDATE GENES; DOWNSTREAM APPLICATIONS; ENDANGERED SPECIES; EPIGENETIC MECHANISMS; EXPRESSION PROFILE; EXPRESSION PROFILING; EXTRACTION METHOD; FILTER SYSTEM; GLOBIN TRANSCRIPTS; HIGH-THROUGHPUT; LARGE POPULATION; NATURAL POPULATION; PROOF OF CONCEPT; RNA EXTRACTION; TRANSCRIPTOMES; WHOLE BLOOD; WILDLIFE SPECIES;

BLOOD; CONSERVATION; CONTAMINATION; DNA; GENE ENCODING; GENE EXPRESSION; HEMOGLOBIN;

RNA;

HEMOGLOBIN; RNA;

ARTICLE; BLOOD SAMPLING; CARNIVORE; DNA CONTAMINATION; NONHUMAN; RNA ANALYSIS; RNA EXTRACTION; RNA SEQUENCE; RNA TRANSCRIPTION; WILDLIFE; ANIMAL; BLOOD; COMPARATIVE STUDY; CONTIG MAPPING; GENETICS; HIGH THROUGHPUT SEQUENCING; ISOLATION AND PURIFICATION; METHODOLOGY; WILD ANIMAL;

VERTEBRATA;

ANIMALS; ANIMALS, WILD; CONTIG MAPPING; HEMOGLOBINS; HIGH-THROUGHPUT NUCLEOTIDE SEQUENCING; RNA;

EID: 84862738008     PISSN: None     EISSN: 14726750     Source Type: Journal    
DOI: 10.1186/1472-6750-12-33     Document Type: Article

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