In-depth analysis of the human tear proteome

Journal of Proteomics

Volumn 75, Issue 13, 2012, Pages 3877-3885

  Zhou, Lei ^a,b   Zhao, Shao Zhen ^c   Koh, Siew Kwan ^a   Chen, Liyan ^a   Vaz, Candida ^d   Tanavde, Vivek ^d   Li, Xiao Rong ^c   Beuerman, Roger W ^a,b,e  

^a SINGAPORE EYE RESEARCH INSTITUTE   (Singapore)

^b NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

^c TIANJIN MEDICAL UNIVERSITY   (China)

^d BIOINFORMATICS INSTITUTE   (Singapore)

^e DUKE NUS MEDICAL SCHOOL   (Singapore)

Author keywords

Body fluids; Human tear proteome; Tear proteomics; TripleTOF

Indexed keywords

ALBUMIN; BIOLOGICAL MARKER; DEFENSIN; ELECTROLYTE; IMMUNOGLOBULIN A; IMMUNOGLOBULIN G; IMMUNOGLOBULIN M; INITIATION FACTOR 4B; INTERLEUKIN 6; LACTOFERRIN; LIPID; LIPOCALIN; LIPOCALIN 1; LYSOZYME; MUCIN 1; MUCIN 16; MUCIN 4; MUCIN 5AC; PEPTIDE DERIVATIVE; PHOSPHOLIPASE A2; PLASMA PROTEIN; PROTEOME; SECRETORY COMPONENT; TEAR PROTEIN; TRANSFERRIN; TREFOIL FACTOR 1; TREFOIL FACTOR 3; TREFOIL PEPTIDE; UNCLASSIFIED DRUG; UNINDEXED DRUG; VASCULOTROPIN;

ADULT; ARTICLE; COMPARATIVE STUDY; CONJUNCTIVA DISEASE; CORNEA DISEASE; DRY EYE; FEMALE; FRACTIONATION; HUMAN; INFLAMMATION; LACRIMAL FLUID; LACRIMAL GLAND; MALE; MASS SPECTROMETER; METABOLITE; NORMAL HUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; REVERSED PHASE LIQUID CHROMATOGRAPHY; TANDEM MASS SPECTROMETRY;

ADULT; DATABASES, GENETIC; EYE PROTEINS; FEMALE; HUMANS; LACRIMAL APPARATUS; MALE; MIDDLE AGED; PROTEOME; TEARS;

EID: 84862701990     PISSN: 18743919     EISSN: 18767737     Source Type: Journal    
DOI: 10.1016/j.jprot.2012.04.053     Document Type: Article

References (60)

1. Green-Church K.B., Butovich I., Willcox M., Borchman D., Paulsen F., Barabino S., et al. The international workshop on meibomian gland dysfunction: report of the subcommittee on tear film lipids and lipid-protein interactions in health and disease. Invest Ophthalmol Vis Sci 2011, 52(4):1979-1993.
2. Green-Church K.B., Nichols K.K., Kleinholz N.M., Zhang L., Nichols J.J. Investigation of the human tear film proteome using multiple proteomic approaches. Mol Vis 2008, 14:456-470.
3. Zhou L., Beuerman R.W., Chan C.M., Zhao S.Z., Li X.R., Yang H., et al. Identification of tear fluid biomarkers in dry eye syndrome using iTRAQ quantitative proteomics. J Proteome Res 2009, 8(11):4889-4905.
4. Tong L., Zhou L., Beuerman R.W., Zhao S.Z., Li X.R. Association of tear proteins with Meibomian gland disease and dry eye symptoms. Br J Ophthalmol 2011, 95(6):848-852.
5. Wong T.T., Zhou L., Li J., Tong L., Zhao S.Z., Li X.R., et al. Proteomic profiling of inflammatory signaling molecules in the tears of patients on chronic glaucoma medication. Invest Ophthalmol Vis Sci 2011, 52(10):7385-7391.
6. Zhou L., Beuerman R.W., Foo Y., Liu S., Ang L.P., Tan D.T. Characterisation of human tear proteins using high-resolution mass spectrometry. Ann Acad Med Singapore 2006, 35(6):400-407.
7. de Souza G.A., Godoy L.M., Mann M. Identification of 491 proteins in the tear fluid proteome reveals a large number of proteases and protease inhibitors. Genome Biol 2006, 7(8):R72.
8. Li N., Wang N., Zheng J., Liu X.M., Lever O.W., Erickson P.M., et al. Characterization of human tear proteome using multiple proteomic analysis techniques. J Proteome Res 2005, 4(6):2052-2061.
9. Chen L., Zhou L., Chan E.C., Neo J., Beuerman R.W. Characterization of the human tear metabolome by LC-MS/MS. J Proteome Res 2011, 10(10):4876-4882.
10. Rantamäki A.H., Seppänen-Laakso T., Oresic M., Jauhiainen M., Holopainen J.M. Human tear fluid lipidome: from composition to function. PLoS One 2011, 6(5):e19553.
11. Zhou L., Beuerman R.W., Huang L., Barathi A., Foo Y.H., Li S.F., et al. Proteomic analysis of rabbit tear fluid: defensin levels after an experimental corneal wound are correlated to wound closure. Proteomics 2007, 7(17):3194-3206.
12. Zhou L., Beuerman R.W., Barathi A., Tan D. Analysis of rabbit tear proteins by high-pressure liquid chromatography/electrospray ionization mass spectrometry. Rapid Commun Mass Spectrom 2003, 17(5):401-412.
13. Grus F.H., Podust V.N., Bruns K., Lackner K., Fu S., Dalmasso E.A., et al. SELDI-TOF-MS proteinchip array profiling of tears from patients with dry eye. Invest Ophthalmol Vis Sci 2005, 46(3):863-876.
14. Versura P., Nanni P., Bavelloni A., Blalock W.L., Piazzi M., Roda A., et al. Tear proteomics in evaporative dry eye disease. Eye (Lond) 2010, 24(8):1396-1402.
15. Koo B.S., Lee D.Y., Ha H.S., Kim J.C., Kim C.W. Comparative analysis of the tear protein expression in blepharitis patients using two-dimensional electrophoresis. J Proteome Res 2005, 4(3):719-724.
16. Tomosugi N., Kitagawa K., Takahashi N., Sugai S., Ishikawa I. Diagnostic potential of tear proteomic patterns in Sjogren's syndrome. J Proteome Res 2005, 4(3):820-825.
17. Acera A., Suarez T., Rodriguez-Agirretxe I., Vecino E., Duran J.A. Changes in tear protein profile in patients with conjunctivochalasis. Cornea 2011, 30(1):42-49.
18. Lema I., Brea D., Rodriguez-Gonzalez R., Diez-Feijoo E., Sobrino T. Proteomic analysis of the tear film in patients with keratoconus. Mol Vis 2010, 16:2055-2061.
19. Pannebaker C., Chandler H.L., Nichols J.J. Tear proteomics in keratoconus. Mol Vis 2010, 16:1949-1957.
20. Okrojek R., Grus F.H., Matheis N., Kahaly G.J. Proteomics in autoimmune thyroid eye disease. Horm Metab Res 2009, 41(6):465-470.
21. Huang da W., Sherman B.T., Lempicki R.A. Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources. Nat Protoc 2009, 4(1):44-57.
22. Andrews G.L., Simons B.L., Young J.B., Hawkridge A.M., Muddiman D.C. Performance characteristics of a new hybrid quadrupole time-of-flight tandem mass spectrometer (TripleTOF 5600). Anal Chem 2011, 83(13):5442-5446.
23. Kocher T., Swart R., Mechtler K. Ultra-high-pressure RPLC hyphenated to an LTQ-Orbitrap Velos reveals a linear relation between peak capacity and number of identified peptides. Anal Chem 2011, 83(7):2699-2704.
24. Zhou L., Huang L.Q., Beuerman R.W., Grigg M.E., Li S.F., Chew F.T., et al. Proteomic analysis of human tears: defensin expression after ocular surface surgery. J Proteome Res 2004, 3(3):410-416.
25. Masinick S.A., Montgomery C.P., Montgomery P.C., Hazlett L.D. Secretory IgA inhibits Pseudomonas aeruginosa binding to cornea and protects against keratitis. Invest Ophthalmol Vis Sci 1997, 38(5):910-918.
26. Fluckinger M., Haas H., Merschak P., Glasgow B.J., Redl B. Human tear lipocalin exhibits antimicrobial activity by scavenging microbial siderophores. Antimicrob Agents Chemother 2004, 48(9):3367-3372.
27. Farrah T., Deutsch E.W., Omenn G.S., Campbell D.S., Sun Z., Bletz J.A., et al. A high-confidence human plasma proteome reference set with estimated concentrations in PeptideAtlas. Mol Cell Proteomics 2011, 10(9). [M110 006353].
28. Sathe S., Sakata M., Beaton A.R., Sack R.A. Identification, origins and the diurnal role of the principal serine protease inhibitors in human tear fluid. Curr Eye Res 1998, 17(4):348-362.
29. Sack R.A., Beaton A., Sathe S., Morris C., Willcox M., Bogart B. Towards a closed eye model of the pre-ocular tear layer. Prog Retin Eye Res 2000, 19(6):649-668.
30. Wistow G., Peterson K., Gao J., Buchoff P., Jaworski C., Bowes-Rickman C., et al. NEIBank: genomics and bioinformatics resources for vision research. Mol Vis 2008, 14:1327-1337.
31. Ozyildirim A.M., Wistow G.J., Gao J., Wang J., Dickinson D.P., Frierson H.F., et al. The lacrimal gland transcriptome is an unusually rich source of rare and poorly characterized gene transcripts. Invest Ophthalmol Vis Sci 2005, 46(5):1572-1580.
32. Sanghi S., Kumar R., Lumsden A., Dickinson D., Klepeis V., Trinkaus-Randall V., et al. cDNA and genomic cloning of lacritin, a novel secretion enhancing factor from the human lacrimal gland. J Mol Biol 2001, 310(1):127-139.
33. McKown R.L., Wang N., Raab R.W., Karnati R., Zhang Y., Williams P.B., et al. Lacritin and other new proteins of the lacrimal functional unit. Exp Eye Res 2009, 88(5):848-858.
34. Fung K.Y., Morris C., Sathe S., Sack R., Duncan M.W. Characterization of the in vivo forms of lacrimal-specific proline-rich proteins in human tear fluid. Proteomics 2004, 4(12):3953-3959.
35. Aho H.J., Saari K.M., Kallajoki M., Nevalainen T.J. Synthesis of group II phospholipase A2 and lysozyme in lacrimal glands. Invest Ophthalmol Vis Sci 1996, 37(9):1826-1832.
36. Qu X.D., Lehrer R.I. Secretory phospholipase A2 is the principal bactericide for staphylococci and other gram-positive bacteria in human tears. Infect Immun 1998, 66(6):2791-2797.
37. Prause J.U. Serum albumin, serum antiproteases and polymorphonuclear leucocyte neutral collagenolytic protease in the tear fluid of normal healthy persons. Acta Ophthalmol (Copenh) 1983, 61(2):261-271.
38. Barka T., Asbell P.A., van der Noen H., Prasad A. Cystatins in human tear fluid. Curr Eye Res 1991, 10(1):25-34.
39. Jumblatt M.M., Imbert Y., Young W.W., Foulks G.N., Steele P.S., Demuth D.R. Glycoprotein 340 in normal human ocular surface tissues and tear film. Infect Immun 2006, 74(7):4058-4063.
40. Ohashi Y., Ishida R., Kojima T., Goto E., Matsumoto Y., Watanabe K., et al. Abnormal protein profiles in tears with dry eye syndrome. Am J Ophthalmol 2003, 136(2):291-299.
41. Ding C., Nandoskar P., Lu M., Thomas P., Trousdale M.D., Wang Y. Changes of aquaporins in the lacrimal glands of a rabbit model of Sjogren's syndrome. Curr Eye Res 2011, 36(6):571-578.
42. Hirai S., Ishida N., Watanabe K., Mita S. Leakage of aquaporin 5 in the tear of dacryoadenitis mice. Invest Ophthalmol Vis Sci 2000, 41(9):2432-2437.
43. Spurr-Michaud S., Argueso P., Gipson I. Assay of mucins in human tear fluid. Exp Eye Res 2007, 84(5):939-950.
44. Mantelli F., Argueso P. Functions of ocular surface mucins in health and disease. Curr Opin Allergy Clin Immunol 2008, 8(5):477-483.
45. Argueso P., Balaram M., Spurr-Michaud S., Keutmann H.T., Dana M.R., Gipson I.K. Decreased levels of the goblet cell mucin MUC5AC in tears of patients with Sjogren syndrome. Invest Ophthalmol Vis Sci 2002, 43(4):1004-1011.
46. Paulsen F.P., Berry M.S. Mucins and TFF peptides of the tear film and lacrimal apparatus. Prog Histochem Cytochem 2006, 41(1):1-53.
47. Ananthi S., Chitra T., Bini R., Prajna N.V., Lalitha P., Dharmalingam K. Comparative analysis of the tear protein profile in mycotic keratitis patients. Mol Vis 2008, 14:500-507.
48. Shankardas J., Senchyna M., Dimitrijevich S.D. Presence and distribution of 14-3-3 proteins in human ocular surface tissues. Mol Vis 2008, 14:2604-2615.
49. Li D.Q., Shang T.Y., Kim H.S., Solomon A., Lokeshwar B.L., Pflugfelder S.C. Regulated expression of collagenases MMP-1, -8, and -13 and stromelysins MMP-3, -10, and -11 by human corneal epithelial cells. Invest Ophthalmol Vis Sci 2003, 44(7):2928-2936.
50. Acera A., Rocha G., Vecino E., Lema I., Duran J.A. Inflammatory markers in the tears of patients with ocular surface disease. Ophthalmic Res 2008, 40(6):315-321.
51. Saghizadeh M., Brown D.J., Castellon R., Chwa M., Huang G.H., Ljubimova J.Y., et al. Overexpression of matrix metalloproteinase-10 and matrix metalloproteinase-3 in human diabetic corneas: a possible mechanism of basement membrane and integrin alterations. Am J Pathol 2001, 158(2):723-734.
52. Laurie G.W., Olsakovsky L.A., Conway B.P., McKown R.L., Kitagawa K., Nichols J.J. Dry eye and designer ophthalmics. Optom Vis Sci 2008, 85(8):643-652.
53. Fullard R.J., Snyder C. Protein levels in nonstimulated and stimulated tears of normal human subjects. Invest Ophthalmol Vis Sci 1990, 31(6):1119-1126.
54. Saari K.M., Aho V., Paavilainen V., Nevalainen T.J. Group II PLA(2) content of tears in normal subjects. Invest Ophthalmol Vis Sci 2001, 42(2):318-320.
55. Vesaluoma M., Teppo A.M., Gronhagen-Riska C., Tervo T. Release of TGF-beta 1 and VEGF in tears following photorefractive keratectomy. Curr Eye Res 1997, 16(1):19-25.
56. Lam H., Bleiden L., de Paiva C.S., Farley W., Stern M.E., Pflugfelder S.C. Tear cytokine profiles in dysfunctional tear syndrome. Am J Ophthalmol 2009, 147(2):198-205. e1.
57. Zhang J.J., Xu L.X., Liu G., Zhao M.H., Wang H.Y. The level of serum secretory IgA of patients with IgA nephropathy is elevated and associated with pathological phenotypes. Nephrol Dial Transplant 2008, 23(1):207-212.
58. Grobmyer S.R., Barie P.S., Nathan C.F., Fuortes M., Lin E., Lowry S.F., et al. Secretory leukocyte protease inhibitor, an inhibitor of neutrophil activation, is elevated in serum in human sepsis and experimental endotoxemia. Crit Care Med 2000, 28(5):1276-1282.
59. Larsson A., Skoldenberg E., Ericson H. Serum and plasma levels of FGF-2 and VEGF in healthy blood donors. Angiogenesis 2002, 5(1-2):107-110.
60. Plomgaard P., Nielsen A.R., Fischer C.P., Mortensen O.H., Broholm C., Penkowa M., et al. Associations between insulin resistance and TNF-alpha in plasma, skeletal muscle and adipose tissue in humans with and without type 2 diabetes. Diabetologia 2007, 50(12):2562-2571.