Fed-batch microbioreactor platform for scale down and analysis of a plasmid DNA production process

Biotechnology and Bioengineering

Volumn 109, Issue 8, 2012, Pages 1976-1986

  Bower, Diana M ^a   Lee, Kevin S ^b   Ram, Rajeev J ^b   Prather, Kristala L J ^a  

^a Massachusetts Institute of Technology Cambridge   (United States)

^b MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

Author keywords

Bioprocess development; Escherichia coli; Microbioreactors; Plasmid biopharmaceuticals

Indexed keywords

ADVANCED MONITORING; BATCH GROWTH; BIOPHARMACEUTICALS; BIOPROCESS DEVELOPMENT; BIOPROCESSES; CONTINUOUS MONITORING; COPY NUMBER; FED BATCHES; FEED-RATES; HIGH-THROUGHPUT; KEY PROCESS; MICRO BIOREACTOR; MICRO-SCALES; MICROBIAL PROCESS; MODEL SYSTEM; MONITORING AND CONTROL; OPERATION SCHEMES; PLASMID DNA; POTENTIAL APPLICATIONS; PREDICTIVE ABILITIES; PROCESS DEVELOPMENT; RESEARCH AND DEVELOPMENT; SCALE DOWN;

BIOMOLECULES; DNA; ESCHERICHIA COLI; GLYCEROL; VACCINES;

BIOREACTORS;

ACETIC ACID; DISSOLVED OXYGEN; GLYCEROL; PLASMID DNA;

AGAR GEL ELECTROPHORESIS; ARTICLE; BIOREACTOR DESIGN; CELL GROWTH; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; DNA VECTOR; ESCHERICHIA COLI; FED BATCH REACTOR; GENE DOSAGE; MOLECULAR MODEL; NONHUMAN; ONLINE MONITORING; OPTICAL DENSITY; PH; PREDICTION; PROCESS DESIGN; PROCESS DEVELOPMENT; QUALITY CONTROL; REACTOR MONITORING; TEMPERATURE;

BIOMASS; CULTURE MEDIA; DNA, BACTERIAL; ESCHERICHIA COLI; GENETIC VECTORS; HYDROGEN-ION CONCENTRATION; OXYGEN; PLASMIDS; SPECTROPHOTOMETRY; TEMPERATURE;

ESCHERICHIA COLI;

EID: 84862689354     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.24498     Document Type: Article

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