Multicenter evaluation of the LightCycler MRSA advanced test, the Xpert MRSA assay, and MRSASelect directly plated culture with simulated workflow comparison for the detection of methicillin-resistant Staphylococcus aureus in nasal swabs

Journal of Molecular Diagnostics

Volumn 14, Issue 4, 2012, Pages 367-375

  Arcenas, Rodney C ^a   Spadoni, Stacey ^b   Mohammad, Amin ^c   Kiechle, Frederick L ^a   Walker, Kimberly ^c   Fader, Robert C ^c   Perdreau Remington, Francoise ^d   Osiecki, John ^e   Liesenfeld, Oliver ^f   Hendrickson, Shelby ^c   Rao, Arundhati ^c  

^a MEMORIAL REGIONAL HOSPITAL   (United States)

^b HINES VA HOSPITAL   (United States)

^c TEXAS A AND M HEALTH SCIENCE CENTER   (United States)

^d UNIVERSITY OF CALIFORNIA   (United States)

^e Roche Diagnostics Corporation   (United States)

^f Medical and Scientific Affairs   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; BACTERIAL COLONIZATION; BACTERIUM CULTURE; BACTERIUM DETECTION; BACTERIUM ISOLATE; BIOASSAY; FALSE POSITIVE RESULT; HUMAN; LIGHTCYCLER MRSA ADVANCED TEST; METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS; MULTICENTER STUDY; NONHUMAN; NOSE SMEAR; SENSITIVITY AND SPECIFICITY; WORKFLOW; XPERT MRSA ASSAY;

HUMANS; METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS; NOSE; STAPHYLOCOCCAL INFECTIONS; WORKFLOW;

EID: 84862656824     PISSN: 15251578     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jmoldx.2012.01.015     Document Type: Article

References (34)

1. Siegel JD, Rhinehart E, Jackson M, Chiarello L; Healthcare Infection Control Practices Advisory Committee: 2007 Guidelines for Isolation Precautions: Preventing Transmission of Infectious Agents in Healthcare Settings [Internet]. Centers for Disease Control and Prevention. 2007. Available at http://www.cdc.gov/hicpac/pdf/isolation/isolation2007.pdf
2. Laupland KB, Ross T, Gregson DB: Staphylococcus aureus blood-stream infections: risk factors, outcomes, and the influence of methicillin resistance in Calgary, Canada, 2000-2006. J Infect Dis 2008, 198:336-343
3. Naber CK: Staphylococcus aureus bacteremia: epidemiology, pathophysiology, and management strategies. Clin Infect Dis 2009, 48(Suppl 4):S231-S237
4. Wyllie DH, Crook DW, Peto TE: Mortality after Staphylococcus aureus bacteraemia in two hospitals in Oxfordshire, 1997-2003: cohort study. Bmj 2006, 333:281
5. Klevens RM, Morrison MA, Nadle J, Petit S, Gershman K, Ray S, Harrison LH, Lynfield R, Dumyati G, Townes JM, Craig AS, Zell ER, Fosheim GE, McDougal LK, Carey RB, Fridkin SK: Invasive methicillin-resistant Staphylococcus aureus infections in the United States. Jama 2007, 298:1763-1771
6. Francois P, Bento M, Renzi G, Harbarth S, Pittet D, Schrenzel J: Evaluation of three molecular assays for rapid identification of methicillin-resistant Staphylococcus aureus. J Clin Microbiol 2007, 45: 2011-2013
7. David MZ, Daum RS: Community-associated methicillin-resistant Staphylococcus aureus: epidemiology and clinical consequences of an emerging epidemic. Clin Microbiol Rev 2010, 23:616-687
8. Liu C, Graber CJ, Karr M, Diep BA, Basuino L, Schwartz BS, Enright MC, O'Hanlon SJ, Thomas JC, Perdreau-Remington F, Gordon S, Gunthorpe H, Jacobs R, Jensen P, Leoung G, Rumack JS, Chambers HF: A population-based study of the incidence and molecular epidemiology of methicillin-resistant Staphylococcus aureus disease in San Francisco, 2004-2005. Clin Infect Dis 2008, 46:1637-1646
9. Datta R, Huang SS: Risk of infection and death due to methicillin-resistant Staphylococcus aureus in long-term carriers. Clin Infect Dis 2008, 47:176-181
10. Huang SS, Platt R: Risk of methicillin-resistant Staphylococcus aureus infection after previous infection or colonization. Clin Infect Dis 2003, 36:281-285
11. Hacek DM, Paule SM, Thomson RB Jr, Robicsek A, Peterson LR: Implementation of a universal admission surveillance and decolonization program for methicillin-resistant staphylococcus aureus (MRSA) reduces the number of MRSA and total number of S. aureus isolates reported by the clinical laboratory. J Clin Microbiol 2009, 47:3749-3752
12. McGinigle KL, Gourlay ML, Buchanan IB: The use of active surveillance cultures in adult ICUs to reduce methicillin-resistant Staphylococcus aureus-related morbidity, mortality and costs: a systematic review. Clin Infect Dis 2008, 46:1717-1725
13. Tacconelli E: Methicillin-resistant Staphylococcus aureus: source control and surveillance organization. Clin Microbiol Infect 2009, 15:31-38
14. Peterson LR, Diekema DJ: To screen or not to screen for methicillin-resistant Staphylococcus aureus. J Clin Microbiol 2010, 48:683-689
15. Hardy K, Price C, Szczepura A, Gossain S, Davies R, Stallard N, Shabir S, McMurray C, Bradbury A, Hawkey PM: Reduction in the rate of methicillin-resistant Staphylococcus aureus acquisition in surgical wards by rapid screening for colonization: a prospective, cross-over study. Clin Microbiol Infect 2010, 16:333-339
16. Luteijn JM, Hubben GA, Pechlivanoglou P, Bonten MJ, Postma MJ: Diagnostic accuracy of culture-based and PCR-based detection tests for methicillin-resistant Staphylococcus aureus: a meta-analysis. Clin Microbiol Infect 2010, 17:146-154
17. Rossney AS, Herra CM, Brennan GI, Morgan PM, O'Connell B: Evaluation of the Xpert methicillin-resistant Staphylococcus aureus (MRSA) assay using the GeneXpert real-time PCR platform for rapid detection of MRSA from screening specimens. J Clin Microbiol 2008, 46:3285-3290
18. Snyder JW, Munier GK, Johnson CL: Comparison of the BD GeneOhm methicillin-resistant Staphylococcus aureus (MRSA) PCR assay to culture by use of BBL CHROMagar MRSA for detection of MRSA in nasal surveillance cultures from intensive care unit patients. J Clin Microbiol 2010, 48:1305-1309
19. Wolk DM, Picton E, Johnson D, Davis T, Pancholi P, Ginocchio CC, Finegold S, Welch DF, de Boer M, Fuller D, Solomon MC, Rogers B, Mehta MS, Peterson LR: Multicenter evaluation of the Cepheid Xpert methicillin-resistant Staphylococcus aureus (MRSA) test as a rapid screening method for detection of MRSA in nares. J Clin Microbiol 2009, 47:758-764
20. Boyce JM, Havill NL: Comparison of BD GeneOhm methicillin-resistant Staphylococcus aureus (MRSA) PCR versus the CHROMagar MRSA assay for screening patients for the presence of MRSA strains. J Clin Microbiol 2008, 46:350-351
21. Malhotra-Kumar S, Abrahantes JC, Sabiiti W, Lammens C, Vercauteren G, Ieven M, Molenberghs G, Aerts M, Goossens H: Evaluation of chromogenic media for detection of methicillin-resistant Staphylococcus aureus. J Clin Microbiol 2010, 48:1040-1046
22. Malhotra-Kumar S, Van Heirstraetam L, Lee A, Abrahantes JC, Lammens C, Vanhommerig E, Molenberghs G, Aerts M, Harbarth S, Goossens H: Evaluation of molecular assays for rapid detection of methicillin-resistant Staphylococcus aureus. J Clin Microbiol 2010, 48:4598-4601
23. Paule SM, Mehta M, Hacek DM, Gonzalzles TM, Robicsek A, Peterson LR: Chromogenic media vs real-time PCR for nasal surveillance of methicillin-resistant Staphylococcus aureus: impact on detection of MRSA-positive persons. Am J Clin Pathol 2009, 131:532-539
24. van Hal SJ, Stark D, Lockwood B, Marriott D, Harkness J: Methicillin-resistant Staphylococcus aureus (MRSA) detection: comparison of two molecular methods (IDI-MRSA PCR assay and GenoType MRSA Direct PCR assay) with three selective MRSA agars (MRSA ID, MRSASelect, and CHROMagar MRSA) for use with infection-control swabs. J Clin Microbiol 2007, 45:2486-2490
25. Wolk DM, Marx JL, Dominguez L, Driscoll D, Schifman RB: Comparison of MRSASelect Agar, CHROMagar Methicillin-Resistant Staphylococcus aureus (MRSA) Medium, and Xpert MRSA PCR for detection of MRSA in Nares: diagnostic accuracy for surveillance samples with various bacterial densities J Clin Microbiol 2009, 47:3933-3936
26. Mohammad AA, Elefano EC, Leigh D, Stredler D, Okorodudu AO, Petersen JR: Using computer simulation to study the impact of increasing test volume on turn around times of STAT samples on ci8000intergrated chemistry and immunoassay analyzer. Clin Chem 2004, 50:1952-1955
27. Mohammad AA, Okorodudub AO, Petersen JR: Utilization of modeling programs for technology change. Health Technology. Edited by C Lehmann. Washington, DC, AACC Press, 2005, pp 1-20
28. Bode LG, Kluytmans JA, Wertheim HF, Bogaers D, Vandenbroucke-Grauls CM, Roosendaal R, Troelstra A, Box AT, Voss A, van der Tweel I, van Belkum A, Verbrugh HA, Vos MC: Preventing surgical-site infections in nasal carriers of Staphylococcus aureus. N Engl J Med 2010, 362:9-17
29. Creamer E, Dolan A, Sherlock O, Thomas T, Walsh J, Moore J, Smyth E, O'Neill E, Shore A, Sullivan D, Rossney AS, Cunney R, Coleman D, Humphreys H: The effect of rapid screening for methicillin-resistant Staphylococcus aureus (MRSA) on the identification and earlier isolation of MRSA-positive patients. Infect Control Hosp Epidemiol 2010, 31:374-381
30. Katayama Y, Ito T, Hiramatsu K: A new class of genetic element, staphylococcus cassette chromosome mec, encodes methicillin resistance in Staphylococcus aureus. Antimicrob Agents Chemother 2000, 44:1549-1555
31. Peterson LR, Liesenfeld O, Woods CW, Allen SD, Pombo D, Patel PA, Mehta MS, Nicholson B, Fuller D, Onderdonk A: Multicenter evaluation of the LightCycler methicillin-resistant Staphylococcus aureus (MRSA) advanced test as a rapid method for detection of MRSA in nasal surveillance swabs. J Clin Microbiol 2010, 48:1661-1666
32. Wren MW, Carder C, Coen PG, Gant V, Wilson AP: Rapid molecular detection of methicillin-resistant Staphylococcus aureus. J Clin Microbiol 2006, 44:1604-1605
33. Robicsek A, Beaumont JL, Paule SM, Hacek DM, Thomson RB Jr, Kaul KL, King P, Peterson LR: Universal surveillance for methicillin-resistant Staphylococcus aureus in 3 affiliated hospitals. Ann Intern Med 2008, 148:409-418
34. Safdar N, Narans L, Gordon B, Kaki DG: Comparison of culture screening methods for detection of nasal carriage of methicillin-resistant staphylococcus aureus: a prospective study comparing 32 methods. J Clin Microbiol 2003, 41:3163-3166