Evaluation of three molecular assays for rapid identification of methicillin-resistant Staphylococcus aureus

Journal of Clinical Microbiology

Volumn 45, Issue 6, 2007, Pages 2011-2013

  Francois, Patrice ^a   Bento, Manuela ^a   Renzi, Gesuele ^a   Harbarth, Stephan ^a   Pittet, Didier ^a   Schrenzel, Jacques ^a  

^a GENEVA UNIVERSITY HOSPITALS   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

PENICILLIN BINDING PROTEIN 2A;

ARTICLE; BACTERIAL CHROMOSOME; BACTERIAL GENE; BACTERIAL GROWTH; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATE; CONTROLLED STUDY; DNA HYBRIDIZATION; GENE AMPLIFICATION; GENE CASSETTE; GENETIC VARIABILITY; METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS; MOLECULAR EPIDEMIOLOGY; NONHUMAN; NUCLEOTIDE SEQUENCE; ORFX GENE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SENSITIVITY AND SPECIFICITY;

BACTERIAL PROTEINS; BACTERIAL TYPING TECHNIQUES; DNA, BACTERIAL; HUMANS; METHICILLIN RESISTANCE; NUCLEIC ACID HYBRIDIZATION; POLYMERASE CHAIN REACTION; REAGENT KITS, DIAGNOSTIC; STAPHYLOCOCCAL INFECTIONS; STAPHYLOCOCCUS AUREUS; TIME FACTORS; TRANSCRIPTION FACTORS;

METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS;

EID: 34250677664     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/JCM.00232-07     Document Type: Article

References (23)

1. Becker, K., I. Pagnier, B. Schuhen, F. Wenzelburger, A. W. Friedrich, F. Kipp, G. Peters, and C. von Eiff. 2006. Does nasal cocolonization by methicillin-resistant coagulase-negative staphylococci and methicillin- susceptible Staphylococcus aureus strains occur frequently enough to represent a risk of false-positive methicillin-resistant S. aureus determinations by molecular methods? J. Clin. Microbiol. 44:229-231.
2. Berglund, C., P. Molling, L. Sjoberg, and B. Soderquist. 2005. Predominance of staphylococcal cassette chromosome mec (SCCmec) type IV among methicillin-resistant Staphylococcus aureus (MRSA) in a Swedish county and presence of unknown SCCmec types with Panton-Valentine leukocidin genes. Clin. Microbiol. Infect. 11:447-456.
3. Bishop, E. J., E. A. Grabsch, S. A. Ballard, B. Mayall, S. Xie, R. Martin, and M. L. Grayson. 2006. Concurrent analysis of nose and groin swab specimens by the IDI-MRSA PCR assay is comparable to analysis by individual-specimen PCR and routine culture assays for detection of colonization by methicillin-resistant Staphylococcus aureus. J. Clin. Microbiol. 44:2904-2908.
4. Chaix, C., I. Durand-Zaleski, C. Alberti, and C. Brun-Buisson. 1999. Control of endemic methicillin-resistant Staphylococcus aureus: a cost-benefit analysis in an intensive care unit. JAMA 282:1745-1751.
5. Clinical and Laboratory Standards Institute. 2005. Performance standards for antimicrobial susceptibility testing: 15th informational supplement M100-S15. Clinical and Laboratory Standards Institute, Wayne, PA.
6. Cuny, C., and W. Witte. 2005. PCR for the identification of methicillin-resistant Staphylococcus aureus (MRSA) strains using a single primer pair specific for SCCmec elements and the neighbouring chromosome-borne orfX. Clin. Microbiol. Infect. 11:834-837.
7. Deplano, A., P. T. Tassios, Y. Glupczynski, E. Godfroid, and M. J. Struelens. 2000. In vivo deletion of the methicillin resistance mec region from the chromosome of Staphylococcus aureus strains. J. Antimicrob. Chemother. 46:617-620.
8. Desjardins, M., C. Guibord, B. Lalonde, B. Toye, and K. Ramotar. 2006. Evaluation of the IDI-MRSA assay for detection of methicillin-resistant Staphylococcus aureus from nasal and rectal specimens pooled in a selective broth. J. Clin. Microbiol. 44:1219-1223.
9. Donnio, P. Y., D. C. Oliveira, N. A. Faria, N. Wilhelm, A. Le Coustumier, and H. de Lencastre. 2005. Partial excision of the chromosomal cassette containing the methicillin resistance determinant results in methicillin-susceptible Staphylococcus aureus. J. Clin. Microbiol. 43:4191-4193.
10. Drews, S. J., B. M. Willey, N. Kreiswirth, M. Wang, T. Ianes, J. Mitchell, M. Latchford, A. J. McGeer, and K. C. Katz. 2006. Verification of the IDI-MRSA assay for detecting methicillin-resistant Staphylococcus aureus in diverse specimen types in a core clinical laboratory setting. J. Clin. Microbiol. 44:3794-3796.
11. Francois, P., A. Huyghe, Y. Charbonnier, M. Bento, S. Herzig, I. Topolski, B. Fleury, D. Lew, P. Vaudaux, S. Harbarth, W. Van Leeuwen, A. Van Belkum, D. S. Blanc, D. Pittet, and J. Schrenzel. 2005. Use of an automated multiple-locus, variable-number tandem repeat-based method for rapid and high-throughput genotyping of Staphylococcus aureus isolates. J. Clin. Microbiol. 43:3346-3355.
12. Francois, P., D. Pittet, M. Bento, B. Pepey, P. Vaudaux, D. Lew, and J. Schrenzel. 2003. Rapid detection of methicillin-resistant Staphylococcus aureus directly from sterile or nonstcrile clinical samples by a new molecular assay. J. Clin. Microbiol. 41:254-260.
13. Francois, P., G. Renzi, D. Pittet, M. Bento, D. Lew, S. Harbarth, P. Vaudaux, and J. Schrenzel. 2004. A novel multiplex real-time PCR assay for rapid typing of major staphylococcal cassette chromosome mec elements. J. Clin. Microbiol. 42:3309-3312.
14. Harbarth, S. 2006. Control of endemic methicillin-resistant Staphylococcus aureus-recent advances and future challenges. Clin. Microbiol. Infect. 12: 1154-1162.
15. Harbarth, S., P. Francois, J. Schrenzel, C. Fankhauser-Rodriguez, S. Hugonnet, T. Koessler, A. Huyghe, and D. Pittet. 2005. Community-associated methicillin-resistant Staphylococcus aureus, Geneva, Switzerland. Emerg. Infect. Dis. 11:962-965.
16. Harbarth, S., C. Masuet-Aumatell, J. Schrenzel, P. Francois, C. Akakpo, G. Renzi, J. Pugin, B. Ricou, and D. Pittet. 2006. Evaluation of rapid screening and pre-emptive contact isolation for detecting and controlling methicillin-resistant Staphylococcus aureus in critical care: an interventional cohort study. Crit. Care 10:R25.
17. Heusser, R., M. Ender, B. Berger-Bachi, and N. McCallum. 2007. Mosaic staphylococcal cassette chromosome mec (SCCmec) containing two recombinase loci and a new mec complex, B2. Antimicrob. Agents Chemother. 51:390-393.
18. Huletsky, A., R. Giroux, V. Rossbach, M. Gagnon, M. Vaillancourt, M. Bernier, F. Gagnon, K. Truchon, M. Bastien, F. J. Picard, A. Van Belkum, M. Ouellette, P. H. Roy, and M. G. Bergeron. 2004. New real-time PCR assay for rapid detection of methicillin-resistant Staphylococcus aureus directly from specimens containing a mixture of staphylococci. J. Clin. Microbiol. 42:1875-1884.
19. Ito, T., K. Okuma, X. X. Ma, H. Yuzawa, and K. Hiramatsu. 2003. Insights on antibiotic resistance of Staphylococcus aureus from its whole genome: genomic island SCC. Drug Resist. Update 6:41-52.
20. Kondo, Y., T. Ito, X. X. Ma, S. Watanabe, B. N. Kreiswirth, J. Etienne, and K. Hiramatsu. 2007. Combination of multiplex PCRs for SCCmec type assignment: rapid identification system for mec, ccr, and major differences in junkyard regions. Antimicrob. Agents Chemother. 51:264-274.
21. Moran, G. J., A. Krishnadasan, R. J. Gorwitz, G. E. Fosheim, L. K. McDougal, R. B. Carey, and D. A. Talan. 2006. Methicillin-resistant S. aureus infections among patients in the emergency department. N. Engl. J. Med. 355:666-674.
22. Oberdorfer, K., S. Pohl, M. Frey, K. Heeg, and C. Wendt. 2006. Evaluation of a single-locus real-time polymerase chain reaction as a screening test for specific detection of methicillin-resistant Staphylococcus aureus in ICU patients. Eur. J. Clin. Microbiol. Infect. Dis. 25:657-663.
23. van Griethuysen, A., I. van Loo, A. Van Belkum, C. Vandenbroucke-Grauls, W. Wannet, P. van Keulen, and J. Kluytmans. 2005. Loss of the mecA gene during storage of methicillin-resistant Staphylococcus aureus strains. J. Clin. Microbiol. 43:1361-1365.