Comparison of culture screening methods for detection of nasal carriage of methicillin-resistant Staphylococcus aureus: A prospective study comparing 32 methods

Journal of Clinical Microbiology

Volumn 41, Issue 7, 2003, Pages 3163-3166

  Safdar, Nasia ^a   Narans, Leah ^a   Gordon, Barbara ^a   Maki, Dennis G ^a  

^a UNIVERSITY OF WISCONSIN SCHOOL OF MEDICINE AND PUBLIC HEALTH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AGAR;

ANALYTIC METHOD; ARTICLE; BACTERIUM DETECTION; CLINICAL ARTICLE; CLINICAL TRIAL; CONTROLLED CLINICAL TRIAL; CONTROLLED STUDY; COTTON; DIAGNOSTIC ACCURACY; DIAGNOSTIC VALUE; HIGH RISK POPULATION; HOSPITAL INFECTION; HUMAN; INCUBATION TIME; INOCULATION; INTERMETHOD COMPARISON; METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS; NOSE SMEAR; PRIORITY JOURNAL; PROSPECTIVE STUDY;

BACTERIOLOGICAL TECHNIQUES; CARRIER STATE; CULTURE MEDIA; HUMANS; METHICILLIN RESISTANCE; NASOPHARYNX; PROSPECTIVE STUDIES; STAPHYLOCOCCAL INFECTIONS; STAPHYLOCOCCUS AUREUS;

BACTERIA (MICROORGANISMS); GOSSYPIUM HIRSUTUM; METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS; POSIBACTERIA; STAPHYLOCOCCUS; STAPHYLOCOCCUS AUREUS;

EID: 0038168173     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/JCM.41.7.3163-3166.2003     Document Type: Article

References (35)

1. Allen, J. L., M. E. Cowan, and P. M. Cockroft. 1994. A comparison of three semi-selective media for the isolation of methicillin-resistant Staphylococcus aureus. J. Med. Microbiol. 40:98-101.
2. Balows, A., W. Hausler, and H. Shadomy. 1991. Manual of clinical microbiology, 5th ed. American Society for Microbiology, Washington D.C.
3. Boyce, J. M., M. M. Jackson, G. Pugliese, M. D. Batt, D. Fleming, J. S. Garner, A. I. Hartstein, C. A. Kauffman, M. Simmons, R. Weinstein, et al. 1994. Methicillin-resistant Staphylococcus aureus (MRSA): a briefing for acute care hospitals and nursing facilities. The AHA Technical Panel on Infections Within Hospitals. Infect. Control Hosp. Epidemiol. 15:105-115.
4. Brayshaw, D. P. 1999. Methicillin-resistant Staphylococcus aureus: evaluation of detection techniques on laboratory-passaged organisms. Br. J. Biomed. Sci. 56:170-176.
5. Carbon, C. 1999. Costs of treating infections caused by methicillin-resistant staphylococci and vancomycin-resistant enterococci. J. Antimicrob. Chemother. 44:31-36.
6. Chambers, H. F. 1997. Methicillin resistance in Staphylococci: molecular and biochemical basis and clinical implications. Clin. Microbiol. Rev. 10:781-791.
7. Cookson, B., B. Peters, M. Webster, I. Phillips, M. Rahman, and W. Noble. 1989. Staff carriage of epidemic methicillin-resistant Staphylococcus aureus. J. Clin. Microbiol. 27:1471-1476.
8. Davies, S., and P. M. Zadik. 1997. Comparison of methods for the isolation of methicillin resistant Staphylococcus aureus, J. Clin. Pathol. 50:257-258.
9. Davies, S., P. M. Zadik, C. M. Mason, and S. J. Whittaker. 2000. Methicillin-resistant Staphyloccocus aureus: evaluation of five selective media. Br. J. Biomed. Sci. 57:269-272.
10. Eriksen, N. H., F. Espersen, V. T. Rosdahl, and K. Jensen. 1994. Evaluation of methods for the detection of nasal carriage of Staphylococcus aureus. APMIS 102:407-412.
11. Ford, M., J. D. Perry, I. Robson, S. Morgan, M. G. Holliday, K. E. Orr, and F. K. Gould. 1999. Evaluation of tube coagulase and a fluorogenic substrate for rapid detection of methicillin-resistant Staphylococcus aureus from selective enrichment broth in an outbreak of EMRSA 15. J. Hosp. Infect. 41:133-135.
12. Gardam, M., J. Brunton, B. Willey, A. McGeer, D. Low, and J. Conly. 2001. A blinded comparison of three laboratory protocols for the identification of patients colonized with methicillin-resistant Staphylococcus aureus. Infect. Control Hosp. Epidemiol. 22:152-156.
13. Gold, H. S., and R. C. Moellering, Jr. 1996. Antimicrobial-drug resistance. N. Engl. J. Med. 335:1445-1453.
14. Gorss, E. B. 1992. Prospective, focused surveillance for oxacillin-resistant Staphylococcus aureus, p. 11.15.1-11.15.2. In H. D. Isenberg (ed.), Clinical microbiology procedures handbook. American Society for Microbiology, Washington, D.C.
15. Hicks, N. R., E. P. Moore, and E. W. Williams. 1991. Carriage and community treatment of methicillin-resistant Staphylococcus aureus: what happens to colonized patients after discharge? J. Hosp. Infect. 19:17-24.
16. Jayaratne, P., and C. Rutherford. 1999. Detection of methicillin-resistant Staphylococcus aureus (MRSA) from growth on mannitol salt oxacillin agar using PCR for nosocomial surveillance. Diagn. Microbiol. Infect. Dis. 35: 13-18.
17. Jonas, D., M. Speck, F. D. Daschner, and H. Grundmann. 2002. Rapid PCR-based identification of methicillin-resistant Staphylococcus aureus from screening swabs. J. Clin. Microbiol. 40:1821-1823.
18. Jones, E. M., K. E. Bowker, R. Cooke, R. J. Marshall, D. S. Reeves, and A. P. MacGowan. 1997. Salt tolerance of EMRSA-16 and its effect on the sensitivity of screening cultures. J. Hosp. Infect. 35:59-62.
19. Kampf, G., K. Weist, S. Swidsinski, M. Kegel, and H. Ruden. 1997. Comparison of screening methods to identify methicillin-resistant Staphylococcus aureus. Eur. J. Clin. Microbiol. Infect. Dis. 16:301-307.
20. Lally, R. T., M. N. Ederer, and B. F. Woolfrey. 1985. Evaluation of mannitol salt agar with oxacillin as a screening medium for methicillin-resistant Staphylococcus aureus. J. Clin. Microbiol. 22:501-504.
21. La Zonby, J. G., and M. J. Starzyk. 1986. Screening method for recovery of methicillin-resistant Staphylococcus aureus from primary plates. J. Clin. Microbiol. 24:186-188.
22. Maranan, M. C., B. Moreira, S. Boyle-Vavra, and R. S. Daum. 1997. Antimicrobial resistance in staphylococci: epidemiology, molecular mechanisms, and clinical relevance. Infect. Dis. Clin. N. Am. 11:813-849.
23. Martinez, O. V., T. Cleary, M. Baker, and J. Civetta. 1992. Evaluation of a mannitol-salt-oxacillin-tellurite medium for the isolation of methicillin-resistant Staphylococcus aureus from contaminated sources. Diagn. Microbiol. Infect. Dis. 15:207-211.
24. Merlino, J., R. Gill, and G. J. Robertson. 1996. Application of lipovitellin-salt-mannitol agar for screening, isolation, and presumptive identification of Staphylococcus aureus in a teaching hospital. J. Clin. Microbiol. 34:3012-3015.
25. Milner, A. E. 2000. Isolation of methicillin-resistant Staphylococcus aureus from non-sterile sites: evaluation of a new selective medium. Br. J. Biomed. Sci. 57:114-118.
26. Nakatomi, Y., and J. Sugiyama. 1998. A rapid latex agglutination assay for the detection of penicillin-binding protein 2'. Microbiol. Immunol. 42:739-743.
27. National Committee for Clinical Laboratory Standards. 1990. Performance standards for antimicrobial disk susceptibility tests. Document M2-A4, 4th ed., vol. 10. National Committee for Clinical Laboratory Standards, Villanova, Pa.
28. National Nosocomial Infections Surveillance System Report. 2001. Data summary from January 1992-June 2001, issued August 2001. Am. J. Infect. Control 29:404-421.
29. Perry, P. L., G. W. Coombs, J. D. Boehm, and J. W. Pearman. 1998. A rapid (20 h) solid screening medium for detecting methicillin-resistant Staphylococcus aureus. J. Hosp. Infect. 40:67-72.
30. Safdar, N., and D. G. Maki. 2002. The commonality of risk factors for nosocomial colonization and infection with antimicrobial-resistant Staphylococcus aureus, enterococcus, gram-negative bacilli, clostridium difficile and Candida. Ann. Intern. Med. 136:834-844.
31. Sautter, R. L., W. J. Brown, and L. H. Mattman. 1988. The use of a selective staphylococcal broth v direct plating for the recovery of Staphylococcus aureus. Infect. Control Hosp. Epidemiol. 9:204-205.
32. Troillet, N., Y. Carmeli, M. H. Samore, J. Dakos, K. Eichelberger, P. C. DeGirolami, and A. W. Karchmer. 1998. Carriage of methicillin-resistant Staphylococcus aureus at hospital admission. Infect. Control Hosp. Epidemiol. 19:181-185.
33. Verghese, S., P. Padmaja, P. Sudha, V. Vanitha, and T. Mathew. 1999. Nasal carriage of methicillin resistant Staphylococcus aureus in a cardiovascular tertiary care centre and its detection by Lipovitellin Salt Mannitol Agar. Indian J. Pathol. Microbiol. 42:441-446.
34. Winstanley, T. G., R. Eggington, and R. C. Spencer. 1993. Selective medium for MRSA. J. Clin, Pathol. 46:1140.
35. Zadik, P. M., S. Davies, S. Whittaker, and C. Mason. 2001. Evaluation of a new selective medium for methicillin-resistant Staphyloccocus aureus. J. Med. Microbiol. 50:476-479.