Multicenter evaluation of the cepheid xpert methicillin-resistant Staphylococcus aureus (MRSA) test as a rapid screening method for detection of MRSA in nares

Journal of Clinical Microbiology

Volumn 47, Issue 3, 2009, Pages 758-764

  Wolk, D M ^a   Picton, E ^a   Johnson, D ^a   Davis, T ^b   Pancholi, P ^c   Ginocchio, C C ^d   Finegold, S ^e   Welch, D F ^f,g   De Boer, M ^a   Fuller, D ^b   Solomon, M C ^c   Rogers, B ^g   Mehta, M S ^h   Peterson, L R ^h  

^a UNIVERSITY OF ARIZONA   (United States)

^b INDIANA UNIVERSITY   (United States)

^c THE OHIO STATE UNIVERSITY WEXNER MEDICAL CENTER   (United States)

^d North Shore Long Island Jewish Health System Laboratories ^*  (United States)

^e VETERANS AFFAIRS MEDICAL CENTER   (United States)

^f MEDICAL CITY DALLAS HOSPITAL   (United States)

^g UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER   (United States)

^h EVANSTON NORTHWESTERN HEALTHCARE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; BACTERIAL STRAIN; BACTERIUM CULTURE; BACTERIUM DETECTION; CONTROLLED STUDY; HEALTH CARE SYSTEM; HUMAN; METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS; NONHUMAN; NOSE SMEAR; PREDICTION AND FORECASTING; PREVALENCE; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SCIENTIFIC LITERATURE; SCREENING; SENSITIVITY AND SPECIFICITY; SEQUENCE ANALYSIS; UNITED STATES;

METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS; NUCLEOPOLYHEDROVIRUS;

EID: 62749097995     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/JCM.01714-08     Document Type: Article

References (34)

1. Alonzo, T. A., and M. S. Pepe. 1999. Using a combination of reference tests to assess the accuracy of a new diagnostic test. Stat. Med. 18:2987-3003.
2. Boyce, J. M., and N. L. Havill. 2008. Comparison of BD GeneOhm methicillin-resistant Staphylococcus aureus (MRSA) PCR versus the CHROMagar MRSA assay for screening patients for the presence of MRSA strains.J. Clin. Microbiol. 46:350-351.
3. Carroll, K. C. 2008. Rapid diagnostics for methicillin-resistant Staphylococcus aureus: current status. Mol. Diagn. Ther. 12:15-24.
4. Cherkaoui, A., G. Renzi, P. Francois, and J. Schrenzel. 2007. Comparison of four chromogenic media for culture-based screening of meticillin-resistant Staphylococcus aureus. J. Med. Microbiol. 56:500-503.
5. Conterno, L. O., J. Shymanski, K. Ramotar, B. Toye, C. van Walraven, D. Coyle, and V. R. Roth. 2007. Real-time polymerase chain reaction detection of methicillin-resistant Staphylococcus aureus: impact on nosocomial transmission and costs. Infect. Control Hosp. Epidemiol. 28:1134-1141.
6. Cosgrove, S. E., Y. Qi, K. S. Kaye, S. Harbarth, A. W. Karchmer, and Y. Carmeli. 2005. The impact of methicillin resistance in Staphylococcus aureus bacteremia on patient outcomes: mortality, length of stay, and hospital charges. Infect. Control Hosp. Epidemiol. 26:166-174.
7. Cunningham, R., P. Jenks, J. Northwood, M. Wallis, S. Ferguson, and S. Hunt. 2007. Effect on MRSA transmission of rapid PCR testing of patients admitted to critical care. J. Hosp. Infect. 65:24-28.
8. Department of Health and Human Services. 14 August 2002. Standards for privacy of individually identifiable health information; final rule. 45 CFR Parts 160 and 164. U.S. Government Printing Office, Washington, DC.
9. Diederen, B., I. van Duijn, A. van Belkum, P. Willemse, P. van Keulen, and J. Kluytmans. 2005. Performance of CHROMagar MRSA medium for detection of methicillin-resistant Staphylococcus aureus. J. Clin. Microbiol. 43:1925-1927.
10. Donnio, P. Y., F. Février, P. Bifani, M. Dehem, C. Kervégant, N. Wilhelm, A. L. Gautier-Lerestif, N. Lafforgue, M. Cormier, the MR-MSSA Study Group of the Collège de Bactériologie-Virologie- Hygiène des Hôpitaux de France, and A. Le Coustumier. 2007. Molecular and epidemiological evidence for spread of multiresistant methicillin-susceptible Staphylococcus aureus strains in hospitals. Antimicrob. Agents Chemother. 51:4342-4350.
11. Drews, S. J., B. M. Willey, N. Kreiswirth, M. Wang, T. Ianes, J. Mitchell, M. Latchford, A. J. McGeer, and K. C. Katz. 2006. Verification of the IDIMRSA assay for detecting methicillin-resistant Staphylococcus aureus in diverse specimen types in a core clinical laboratory setting. J. Clin. Microbiol. 44:3794-3796.
12. Edwards, J. R., K. D. Peterson, M. L. Andrus, J. S. Tolson, J. S. Goulding, M. A. Dudeck, R. B. Mincey, D. A. Pollock, T. C. Horan, and the NHSN Facilities. 2007. National Healthcare Safety Network (NHSN) Report, data summary for 2006, issued June 2007. Am. J. Infect. Control 35:290-301.
13. Farley, J. E., P. D. Stamper, T. Ross, M. Cai, S. Speser, and K. C. Carroll. 2008. Comparison of the BD GeneOhm methicillin-resistant Staphylococcus aureus (MRSA) PCR assay to culture by use of BBL CHROMagar MRSA for detection of MRSA in nasal surveillance cultures from an at-risk community population. J. Clin. Microbiol. 46:743-746.
14. Flayhart, D., J. F. Hindler, D. A. Bruckner, G. Hall, R. K. Shrestha, S. A. Vogel, S. S. Richter, W. Howard, R. Walther, and K. C. Carroll. 2005. Multicenter evaluation of BBL CHROMagar MRSA medium for direct detection of methicillin-resistant Staphylococcus aureus from surveillance cultures of the anterior nares. J. Clin. Microbiol. 43:5536-5540.
15. Francois, P., M. Bento, G. Renzi, S. Harbarth, D. Pittet, and J. Schrenzel. 2007. Evaluation of three molecular assays for rapid identification of methicillin-resistant Staphylococcus aureus. J. Clin. Microbiol. 45:2011-2013.
16. Galen, R. S., and T. Peters. 1986. Analytical goals and clinical relevance of laboratory procedures, p. 398. In N. W. Tietz (ed.), Textbook of clinical chemistry. W. B. Saunders, Philadelphia, PA.
17. Hadgu, A. 2000. Discrepant analysis is an inappropriate and unscientific method. J. Clin. Microbiol. 38:4301-4302.
18. Huletsky, A., R. Giroux, V. Rossbach, M. Gagnon, M. Vaillancourt, M. Bernier, F. Gagnon, K. Truchon, M. Bastien, F. J. Picard, A. van Belkum, M. Ouellette, P. H. Roy, and M. G. Bergeron. 2004. New real-time PCR assay for rapid detection of methicillin-resistant Staphylococcus aureus directly from specimens containing a mixture of staphylococci. J. Clin. Microbiol. 42:1875-1884.
19. Huletsky, A., P. Lebel, F. J. Picard, M. Bernier, M. Gagnon, N. Boucher, and M. G. Bergeron. 2005. Identification of methicillin-resistant Staphylococcus aureus carriage in less than 1 hour during a hospital surveillance program. Clin. Infect. Dis. 40:976-981.
20. Khoury, J., M. Jones, A. Grim, W. M. Dunne, Jr., and V. Fraser. 2005. Eradication of methicillin-resistant Staphylococcus aureus from a neonatal intensive care unit by active surveillance and aggressive infection control measures. Infect. Control Hosp. Epidemiol. 26:616-621.
21. Klein, E., D. L. Smith, and R. Laxminarayan. 2007. Hospitalizations and deaths caused by methicillin-resistant Staphylococcus aureus, United States, 1999-2005. Emerg. Infect. Dis. 13:1840-1846.
22. Klevens, R. M., M. A. Morrison, J. Nadle, S. Petit, K. Gershman, S. Ray, L. H. Harrison, R. Lynfield, G. Dumyati, J. M. Townes, A. S. Craig, E. R. Zell, G. E. Fosheim, L. K. McDougal, R. B. Carey, and S. K. Fridkin. 2007. Invasive methicillin-resistant Staphylococcus aureus infections in the United States. JAMA 298:1763-1771.
23. Liassine, N., F. Decosterd, and J. Etienne. 2007. Evaluation of IDI-MRSA assay on a collection of community-acquired methicillin-resistant Staphylococcus aureus isolates and on carriage specimens. Pathol. Biol. (Paris) 55:378-381. (In French.)
24. Mauldin, P. D., C. D. Salgado, V. L. Durkalski, and J. A. Bosso. 2008. Nosocomial infections due to methicillin-resistant Staphylococcus aureus and vancomycin-resistant enterococcus: relationships with antibiotic use and cost drivers. Ann. Pharmacother. 42:317-326.
25. Muto, C. A., J. A. Jernigan, B. E. Ostrowsky, H. M. Richet, W. R. Jarvis, J. M. Boyce, and B. M. Farr. 2003. SHEA guideline for preventing nosocomial transmission of multidrug-resistant strains of Staphylococcus aureus and enterococcus. Infect. Control Hosp. Epidemiol. 24:362-386.
26. Paule, S. M., D. M. Hacek, B. Kufner, K. Truchon, R. B. Thomson, Jr., K. L. Kaul, A. Robicsek, and L. R. Peterson. 2007. Performance of the BD GeneOhm methicillin-resistant Staphylococcus aureus test before and during high-volume clinical use. J. Clin. Microbiol. 45:2993-2998.
27. Peterson, L. R., D. M. Hacek, and A. Robicsek. 2007. Case study: an MRSA intervention at Evanston Northwestern Healthcare. Jt. Comm. J. Qual. Patient Saf. 33:732-738.
28. Robicsek, A., J. L. Beaumont, S. M. Paule, D. M. Hacek, R. B. Thomson, Jr., K. L. Kaul, P. King, and L. R. Peterson. 2008. Universal surveillance for methicillin-resistant Staphylococcus aureus in 3 affiliated hospitals. Ann. Intern. Med. 148:409-418.
29. Rossney, A. S., C. M. Herra, G. I. Brennan, P. M. Morgan, and B. O'Connell. 2008. Evaluation of the Xpert methicillin-resistant Staphylococcus aureus (MRSA) assay using the GeneXpert real-time PCR platform for rapid detection of MRSA from screening specimens. J. Clin. Microbiol. 46:3285-3290.
30. Shurland, S., M. Zhan, D. D. Bradham, and M. C. Roghmann. 2007. Comparison of mortality risk associated with bacteremia due to methicillin-resistant and methicillin-susceptible Staphylococcus aureus. Infect. Control Hosp. Epidemiol. 28:273-279.
31. Spellberg, B., R. Guidos, D. Gilbert, J. Bradley, H. W. Boucher, W. M. Scheld, J. G. Bartlett, and J. Edwards, Jr. 2008. The epidemic of antibiotic-resistant infections: a call to action for the medical community from the Infectious Diseases Society of America. Clin. Infect. Dis. 46:155-164.
32. van Loo, I. H., S. van Dijk, I. Verbakel-Schelle, and A. G. Buiting. 2007. Evaluation of a chromogenic agar (MRSASelect) for the detection of meticillin-resistant Staphylococcus aureus with clinical samples in The Netherlands. J. Med. Microbiol. 56:491-494.
33. van Trijp, M. J., D. C. Melles, W. D. Hendriks, G. A. Parlevliet, M. Gommans, and A. Ott. 2007. Successful control of widespread methicillin-resistant Staphylococcus aureus colonization and infection in a large teaching hospital in the Netherlands. Infect. Control Hosp. Epidemiol. 28:970-975.
34. Wagenvoort, J. H., W. Sluijsmans, and R. J. Penders. 2000. Better environmental survival of outbreak vs. sporadic MRSA isolates. J. Hosp. Infect. 45:231-234.