Establishment of ultra long-lived cell lines by transfection of TERT into normal human fibroblast TIG-1 and their characterization

Cell Biology International

Volumn 36, Issue 6, 2012, Pages 519-527

  Kamada, Mizuna ^a   Kumazaki, Tsutomu ^a   Matsuo, Taira ^a   Mitsui, Youji ^a   Takahashi, Tomoko ^a  

^a TOKUSHIMA BUNRI UNIVERSITY   (Japan)

Author keywords

Cell transplantation; Immortal cell strains; Non tumorigenic; Replicative senescence; Telomere

Indexed keywords

BETA GALACTOSIDASE; COLLAGEN 1ALPHA; COLLAGEN TYPE 1; CYCLIN DEPENDENT KINASE INHIBITOR 1; CYCLIN DEPENDENT KINASE INHIBITOR 2A; FIBRONECTIN; PROTEIN P53; TELOMERASE REVERSE TRANSCRIPTASE; UNCLASSIFIED DRUG;

ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; CELL CLONE; CELL IMMORTALIZATION; CELL LINE; CELL POPULATION; CELL STRAIN TIG 1; CELL STRUCTURE; CELL SURVIVAL; CHROMOSOME LOSS; CONTROLLED STUDY; ENZYME ACTIVITY; FEMALE; FETUS; FIBROBLAST CULTURE; GENE; GENE EXPRESSION REGULATION; GENETIC TRANSFECTION; GROWTH RATE; HUMAN; HUMAN CELL; KARYOTYPING; LUNG FIBROBLAST; MOUSE; NONHUMAN; PHENOTYPIC VARIATION; PROTEIN EXPRESSION; SENESCENCE; TELOMERASE REVERSE TRANSCRIPTASE GENE; TELOMERE; X CHROMOSOME;

ABNORMAL KARYOTYPE; ANIMALS; CELL LINE; CELL SHAPE; CELL SURVIVAL; CELL TRANSFORMATION, NEOPLASTIC; CYCLIN-DEPENDENT KINASE INHIBITOR P21; FEMALE; FIBROBLASTS; GENTAMICINS; HUMANS; MICE; MICE, SCID; RECOMBINANT PROTEINS; TELOMERASE; TELOMERE HOMEOSTASIS; TRANSFECTION; TUMOR SUPPRESSOR PROTEIN P53;

EID: 84862506069     PISSN: 10656995     EISSN: 10958355     Source Type: Journal    
DOI: 10.1042/CBI20110460     Document Type: Article

References (29)

1. Anno K, Hayashi A, Takahashi T, Mitsui Y, Ide T, Tahara H. Telomerase activation induces elongation of the telomeric single-stranded overhang, but does not prevent chromosome aberrations in human vascular endothelial cells. Biochem Biophys Res Commun 2007;353:926-932.
2. Broccoli D, Young JW, de Lange T. Telomerase activity in normal and malignant hematopoietic cells. Proc Natl Acad Sci USA 1995;92:9082-9086.
3. Counter CM, Avilion AA, LeFeuvrel CE, Stewart NG, Greider CW, Harley CB et al. Telomere shortening associated with chromosome instability is arrested in immortal cells which express telomerase activity. EMBO J 1992;1:1921-1929.
4. Cutler C, Antin JH. Peripheral blood stem cells for allogeneic transplantation: a review. Stem Cells 2001;19:108-117.
5. Dickson MA, Hahn WC, Ino Y, Ronfard V, Wu JY, Weinberg RA et al. Human keratinocytes that express hTERT and also bypass a p16INK4a-enforced mechanism that limits life span become immortal yet retain normal growth and differentiation characteristics. Mol Cell Biol 2000;20:1436-1447.
6. Dimri GP, Lee X, Basile G, Acosta M, Scott G, Roskelley C et al. A biomarker that identifies senescent human cells in culture and in aging skin in vivo. Proc Natl Acad Sci USA 1995;92:9363-9367.
7. Grant JD, Broccoli D, Muquit M, Manion FJ, Tisdall J, Ochs MF. Telometric: a tool providing simplified, reproducible measurements of telomeric DNA from constant field agarose gels. BioTechniques 2001;31:1314-1318.
8. Gratwohl A, Baldomero H, Aljurf M, Pasquini MC, Bouzas LF, Yoshimi A et al. Hematopoietic stem cell transplantation: a global perspective. JAMA, J Am Med Assoc 2010;303:1617-1624.
9. Hahn WC, Dessain SK, Brooks MW, King JE, Elenbaas B, Sabatini DM et al. Enumeration of the simian virus 40 early region elements necessary for human cell transformation. Mol Cell Biol 2002;22:2111-2123.
10. Harley CB. Telomerase is not an oncogene. Oncogene 2002;21:494-502.
11. Hayflick L, Moorhead PS. The serial cultivation of human diploid cell strains. Exp Cell Res 1961;25:581-621.
12. Hiyama K, Hirai Y, Kyoizumi S, Akiyama M, Hiyama E, Piatyszek MA et al. Activation of telomerase in human lymphocytes and hematopoietic progenitor cells. J Immunol 1995;155:3711-3715.
13. Hiyama K, Otani K, Ohtaki M, Satoh K, Kumazaki T, Takahashi T et al. Differentially expressed genes throughout the cellularimmortalization processes are quite different between normal human fibroblasts and endothelial cells. Int J Oncol 2005;27:87-95.
14. Kataoka H, Tahara H, Watanabe T, Sugawara M, Ide T, Goto M et al. Immortalization of immunologically committed Epstein-Barr virus-transformed human B-lymphoblastoid cell lines accompanied by a strong telomerase activity. Differentiation 1997;62:203-211.
15. Kiyono T, Foster SA, Koop JI, McDougall JK, Galloway DA, Klingelhutz AJ. Both Rb/p16INK4A inactivation and telomerase activity are required to immortalize human epithelial cells. Nature 1998;396:84-88.
16. Kumakura S, Yamamoto A, Yagisawa J, Uchida M, Tsutsui T. Telomere length and telomerase activity in the process of human fibroblast immortalization. Odontology 2002;90:13-21.
17. Kumamoto H, Kinouchi Y, Ooya K. Telomerase activity and telomerase reverse transcriptase (TERT) expression in ameloblastomas. J Oral Pathol Med 2001;30:231-236.
18. Kumazaki T, Sakano T, Yoshida T, Hamada K, Sumida H, Teranishi Y et al. Enhanced expression of mitochondrial genes in senescent endothelial cells and fibroblasts. Mech Aging Dev 1998;101:91-99.
19. Kumazaki T, Hiyama K, Takahashi T, Omatsu H, Tanimoto K, Noguchi T et al. Differential gene expressions during immortalization of normal human fibroblasts and endothelial cells transfected with human telomerase reverse transcriptase gene. Int J Oncol 2004;24:1435-1442.
20. Kumazaki T, Kurata A, Matsuo T, Mitsui Y, Takahashi T. Establishment of human induced pluripotent stem cell lines from normal fibroblast TIG-1. Human Cell 2011;24:96-103.
21. Martin GM, Sprague CA, Epstein CJ. Replicative life-span of cultivated human cells. Effects of donor's age, tissue, and genotype. Lab Invest 1970;23:86-92.
22. Mitsui Y, Sakagami H. Histon H1 depletion with human cellular aging: biochemical and cytochemical evidences. Acta Histochem Cytochem 1983;16:403-414.
23. Nivison-Smith I, Bradstock KF, Dodds AJ, Hawkins PA, Szer J. Haemopoietic stem cell transplantation in Australia and New Zealand, 1992-2001: progress report from the Australasian Bone Marrow Transplant Recipient Registry. Intern Med J 2005;35:18-27.
24. Notaro R, Cimmino A, Tabarini D, Rotoli B, Luzzatto L. In vivo telomere dynamics of human hematopoietic stem cells. Proc Natl Acad Sci USA 1997;94:1378213785.
25. Ohashi M, Aizawa S, Ooka H, Ohsawa T, Kaji K, Kondo H et al. A new human diploid cell strain, TIG-1, for the research on cellular aging. Exp Gerontol 1980;15:121-133.
26. Pirisi L, Yasumoto S, Feller M, Doninger J, DiPaolo JA. Transformation of human fibroblasts and keratinocytes with human papillomavirus type 16 DNA. J Virol 1987;61:1061-1066.
27. Takahashi T, Kawabe T, Okazaki Y, Itoh C, Noda K, Tajima M et al. In vitro establishment of tumorigenic human B-lymphoblastoid cell lines transformed by Epstein-Barr virus. DNA Cell Biol 2003;22:727-735.
28. Watanabe S, Kanda T, Yoshiike K. Human papillomavirus type 16 transformation of primary human embryonic fibroblasts requires expression of open reading frames E6 and E7. J Virol 1989;63:965-969.
29. Yui J, Chiu CP, Lansdorp PM. Telomerase activity in candidate stem cells from fetal liver and adult bone marrow. Blood 1998;91:3255-3262.