MspA nanopores from subunit dimers

PLoS ONE

Volumn 7, Issue 6, 2012, Pages

  Pavlenok, Mikhail ^a   Derrington, Ian M ^b   Gundlach, Jens H ^b   Niederweis, Michael ^a  

^a University of Alabama at Birmingham   (United States)

^b UNIVERSITY OF WASHINGTON   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DIMER; GLUCOSE; MONOMER; PORIN A; SINGLE STRANDED DNA;

ARTICLE; CHANNEL GATING; COMPLEX FORMATION; CONTROLLED STUDY; DNA HAIRPIN; DNA SEQUENCE; DNA TRANSFER; GLUCOSE TRANSPORT; LIPID BILAYER; MOLECULAR DYNAMICS; MYCOBACTERIUM SMEGMATIS; NANOPORE; NONHUMAN; PHYSICAL CHEMISTRY; PROTEIN DEPLETION; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN FUNCTION; PROTEIN INTERACTION; PROTEIN LOCALIZATION; PROTEIN PURIFICATION; PROTEIN STRUCTURE; PROTEIN SYNTHESIS; SEQUENCE ANALYSIS; STOICHIOMETRY;

DIMERIZATION; ION CHANNEL GATING; LIPID BILAYERS; MODELS, MOLECULAR; NANOSTRUCTURES; PORINS;

EID: 84862505696     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0038726     Document Type: Article

References (40)

1. Iacovache I, Bischofberger M, van der Goot FG, (2010) Structure and assembly of pore-forming proteins. Curr Opin Struct Biol 20: 241-246.
2. Nikaido H, (2003) Molecular basis of bacterial outer membrane permeability revisited. Microbiol Mol Biol Rev 67: 593-656.
3. Bayley H, Cremer PS, (2001) Stochastic sensors inspired by biology. Nature 413: 226-230.
4. Rotem D, Jayasinghe L, Salichou M, Bayley H, (2012) Protein detection by nanopores equipped with aptamers. J Am Chem Soc 134: 2781-2787.
5. Branton D, Deamer DW, Marziali A, Bayley H, Benner SA, et al. (2008) The potential and challenges of nanopore sequencing. Nat Biotechnol 26: 1146-1153.
6. Gouaux E, (1998) a-Hemolysin from Staphylococcus aureus: an archetype of b-barrel, channel-forming toxins. J Struct Biol 121: 110-122.
7. Faller M, Niederweis M, Schulz GE, (2004) The structure of a mycobacterial outer-membrane channel. Science 303: 1189-1192.
8. Stahl C, Kubetzko S, Kaps I, Seeber S, Engelhardt H, et al. (2001) MspA provides the main hydrophilic pathway through the cell wall of Mycobacterium smegmatis. Mol Microbiol 40: 451-464 (Authors' correction appeared in Mol. Microbiol. 457, 1509).
9. Niederweis M, (2003) Mycobacterial porins- new channel proteins in unique outer membranes. Mol Microbiol 49: 1167-1177.
10. Heinz C, Niederweis M, (2000) Selective extraction and purification of a mycobacterial outer membrane protein. Anal Biochem 285: 113-120.
11. Derrington IM, Butler TZ, Collins MD, Manrao E, Pavlenok M, et al. (2010) Nanopore DNA sequencing with MspA. Proc Natl Acad Sci U S A 107: 16060-16065.
12. Marchler-Bauer A, Lu S, Anderson JB, Chitsaz F, Derbyshire MK, et al. (2011) CDD: a Conserved Domain Database for the functional annotation of proteins. Nucleic Acids Res 39: D225-229.
13. Nassi S, Collier RJ, Finkelstein A, (2002) PA63 channel of anthrax toxin: an extended beta-barrel. Biochemistry 41: 1445-1450.
14. Kintzer AF, Thoren KL, Sterling HJ, Dong KC, Feld GK, et al. (2009) The protective antigen component of anthrax toxin forms functional octameric complexes. J Mol Biol 392: 614-629.
15. Wörner M, Lioubashevski O, Basel MT, Niebler S, Gogritchiani E, et al. (2007) Characterization of nanostructured surfaces generated by reconstitution of the porin MspA from Mycobacterium smegmatis. Small 3: 1084-1097.
16. Hammerstein AF, Jayasinghe L, Bayley H, (2011) Subunit dimers of alpha-hemolysin expand the engineering toolbox for protein nanopores. J Biol Chem 286: 14324-14334.
17. Mahfoud M, Sukumaran S, Hülsmann P, Grieger K, Niederweis M, (2006) Topology of the porin MspA in the outer membrane of Mycobacterium smegmatis. J Biol Chem 281: 5908-5915.
18. Huston JS, Levinson D, Mudgett-Hunter M, Tai MS, Novotny J, et al. (1988) Protein engineering of antibody binding sites: recovery of specific activity in an anti-digoxin single-chain Fv analogue produced in Escherichia coli. Proc Natl Acad Sci U S A 85: 5879-5883.
19. Stephan J, Bender J, Wolschendorf F, Hoffmann C, Roth E, et al. (2005) The growth rate of Mycobacterium smegmatis depends on sufficient porin-mediated influx of nutrients. Mol Microbiol 58: 714-730.
20. Niederweis M, Ehrt S, Heinz C, Klöcker U, Karosi S, et al. (1999) Cloning of the mspA gene encoding a porin from Mycobacterium smegmatis. Mol Microbiol 33: 933-945.
21. Butler TZ, Pavlenok M, Derrington IM, Niederweis M, Gundlach JH, (2008) Single-molecule DNA detection with an engineered MspA protein nanopore. Proc Natl Acad Sci U S A 105: 20647-20652.
22. Heinz C, Roth E, Niederweis M, (2003) Purification of porins from Mycobacterium smegmatis. Methods Mol Biol 228: 139-150.
23. Liu N, Delcour AH, (1998) The spontaneous gating activity of OmpC porin is affected by mutations of a putative hydrogen bond network or of a salt bridge between the L3 loop and the barrel. Protein Eng 11: 797-802.
24. Bainbridge G, Gokce I, Lakey JH, (1998) Voltage gating is a fundamental feature of porin and toxin beta-barrel membrane channels. FEBS Lett 431: 305-308.
25. Robertson KM, Tieleman DP, (2002) Molecular basis of voltage gating of OmpF porin. Biochem Cell Biol 80: 517-523.
26. Huff J, Pavlenok M, Sukumaran S, Niederweis M, (2009) Functions of the periplasmic loop of the porin MspA from Mycobacterium smegmatis. J Biol Chem 284: 10223-10231.
27. Mailaender C, Reiling N, Engelhardt H, Bossmann S, Ehlers S, et al. (2004) The MspA porin promotes growth and increases antibiotic susceptibility of both Mycobacterium bovis BCG and Mycobacterium tuberculosis. Microbiology 150: 853-864.
28. Kavoosi M, Creagh AL, Kilburn DG, Haynes CA, (2007) Strategy for selecting and characterizing linker peptides for CBM9-tagged fusion proteins expressed in Escherichia coli. Biotechnol Bioeng 98: 599-610.
29. Alfthan K, Takkinen K, Sizmann D, Soderlund H, Teeri TT, (1995) Properties of a single-chain antibody containing different linker peptides. Protein Eng 8: 725-731.
30. Guo XV, Monteleone M, Klotzsche M, Kamionka A, Hillen W, et al. (2007) Silencing Mycobacterium smegmatis by using tetracycline repressors. J Bacteriol 189: 4614-4623.
31. Economou A, (1999) Following the leader: bacterial protein export through the Sec pathway. Trends Microbiol 7: 315-320.
32. Engelhardt H, Heinz C, Niederweis M, (2002) A tetrameric porin limits the cell wall permeability of Mycobacterium smegmatis. J Biol Chem 277: 37567-37572.
33. Nikaido H, Rosenberg EY, (1983) Porin channels in Escherichia coli: studies with liposomes reconstituted from purified proteins. J Bacteriol 153: 241-252.
34. de la Vega AL, Delcour AH, (1995) Cadaverine induces closing of E. coli porins. EMBO J 14: 6058-6065.
35. Iyer R, Delcour AH, (1997) Complex inhibition of OmpF and OmpC bacterial porins by polyamines. J Biol Chem 272: 18595-18601.
36. Müller DJ, Engel A, (1999) Voltage and pH-induced channel closure of porin OmpF visualized by atomic force microscopy. J Mol Biol 285: 1347-1351.
37. Duret G, Simonet V, Delcour AH, (2007) Modulation of Vibrio cholerae porin function by acidic pH. Channels 1: 70-79.
38. Tang Y, Jiang N, Parakh C, Hilvert D, (1996) Selection of linkers for a catalytic single-chain antibody using phage display technology. J Biol Chem 271: 15682-15686.
39. Heinz C, Karosi S, Niederweis M, (2003) High level expression of the mycobacterial porin MspA in Escherichia coli and purification of the recombinant protein. J Chromatogr B 790: 337-348.
40. Bi W, Stambrook PJ, (1997) CCR: a rapid and simple approach for mutation detection. Nucleic Acids Res 25: 2949-2951.