Hydroxy-fatty acid production in a Pseudomonas aeruginosa 42A2 PHA synthase mutant generated by directed mutagenesis

Applied Microbiology and Biotechnology

Volumn 93, Issue 6, 2012, Pages 2551-2561

  Torrego Solana, Noelia ^a   Martin Arjol, Ignacio ^a   Bassas Galia, Mònica ^b   Diaz, Pilar ^a   Manresa, Angeles ^a  

^a UNIVERSITY OF BARCELONA   (Spain)

^b HELMHOLTZ CENTRE FOR INFECTION RESEARCH   (Germany)

Author keywords

Cre lox antibiotic recycling; Hydroxy fatty acids; PHA negative mutant; Pseudomonas aeruginosa; SacB based negative selection

Indexed keywords

ACID PRODUCTION; CRE LOXES; DELETION MUTANTS; ENZYMATIC CONVERSIONS; HIGHER EFFICIENCY; MARKER RECYCLING; MUTANT STRAIN; NEGATIVE SELECTION; OXYGEN UPTAKE RATES; P.AERUGINOSA; PHA SYNTHASES; PHA-NEGATIVE MUTANT; PSEUDOMONAS AERUGINOSA; WASTE FRYING OIL; WILD-TYPE STRAIN;

ANTIBIOTICS; BIOCHEMISTRY; BIOSYNTHESIS; OLEIC ACID; RECYCLING; TRANSMISSION ELECTRON MICROSCOPY;

BACTERIA;

CARBON; GENTAMICIN; HYDROXY FATTY ACID; OLEIC ACID; OLIVE OIL; OXYGEN; POLYHYDROXYALKANOATE SYNTHASE; SUNFLOWER OIL; ACYLTRANSFERASE; BACTERIAL PROTEIN; FATTY ACID; POLY(3-HYDROXYALKANOIC ACID) SYNTHASE; POLYHYDROXYALKANOIC ACID;

ANTIBIOTICS; BACTERIUM; DOMESTIC WASTE; ENZYME ACTIVITY; FATTY ACID; NUCLEAR MAGNETIC RESONANCE; TRANSMISSION ELECTRON MICROSCOPY; VEGETABLE OIL;

ARTICLE; BACTERIUM ISOLATION; BACTERIUM MUTANT; BIOMASS; BIOREACTOR; CHEMICAL REACTION; CHROMATOGRAPHY; ENZYME LOCALIZATION; FATTY ACID SYNTHESIS; IMAGE ANALYSIS; INOCULATION; LIQUID CHROMATOGRAPHY; MOLECULAR IMAGING; MUTAGENESIS; NONHUMAN; NUCLEAR MAGNETIC RESONANCE IMAGING; OXYGEN CONSUMPTION; PSEUDOMONAS AERUGINOSA; TRANSMISSION ELECTRON MICROSCOPY; BIOSYNTHESIS; ENZYMOLOGY; GENETICS; METABOLISM; SITE DIRECTED MUTAGENESIS;

PSEUDOMONAS AERUGINOSA;

ACYLTRANSFERASES; BACTERIAL PROTEINS; FATTY ACIDS; MUTAGENESIS, SITE-DIRECTED; POLYHYDROXYALKANOATES; PSEUDOMONAS AERUGINOSA;

EID: 84862292949     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-011-3646-z     Document Type: Article

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