Biochemical evidence that phaZ gene encodes a specific intracellular medium chain length polyhydroxyalkanoate depolymerase in Pseudomonas putida KT2442: Characterization of a paradigmatic enzyme

Journal of Biological Chemistry

Volumn 282, Issue 7, 2007, Pages 4951-4962

  De Eugenio, Laura I ^a   García, Pedro ^a   Luengo, José M ^b   Sanz, Jesús M ^c   Román, Julio San ^d   García, José Luis ^a   Prieto, María A ^a  

^a CENTRO DE INVESTIGACIONES BIOLÓGICAS   (Spain)

^b UNIVERSITY OF LEÓN   (Spain)

^c MIGUEL HERNÁNDEZ UNIVERSITY   (Spain)

^d INSTITUTO DE CIENCIA Y TECNOLOGÍA DE POLÍMEROS   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

BIOCHEMISTRY; DEPOLYMERIZATION; ENZYME ACTIVITY; ENZYME KINETICS; MICROORGANISMS; MUTAGENESIS;

INTRACELLULAR MEDIUM CHAIN LENGTH; PARADIGMATIC ENZYME; POLYHYDROXYALKANOATE DEPOLYMERASE; PSEUDOMONAS PUTIDA STRAINS;

GENE ENCODING;

ALIPHATIC COMPOUND; AROMATIC COMPOUND; BENZYLSULFONYL FLUORIDE; GENE PRODUCT; HYDROLASE; MONOMER; NUCLEOTIDYLTRANSFERASE; POLYHYDROXYALKANOATE Z DEPOLYMERASE; SERINE DEHYDRATASE; UNCLASSIFIED DRUG; BACTERIAL PROTEIN; CARBOXYLESTERASE; POLY(3 HYDROXYALKANOIC ACID) DEPOLYMERASE; POLY(3-HYDROXYALKANOIC ACID) DEPOLYMERASE; RECOMBINANT PROTEIN;

ARTICLE; CELL GRANULE; CELLULAR DISTRIBUTION; COMPLEX FORMATION; DEPOLYMERIZATION; ENZYME ACTIVE SITE; ENZYME ANALYSIS; ENZYME PURIFICATION; ESCHERICHIA COLI; GENE; GENE OVEREXPRESSION; GENE STRUCTURE; HYDROLYSIS; IN VITRO STUDY; INTRACELLULAR SPACE; NONHUMAN; NUCLEOTIDE SEQUENCE; PHAZ GENE; PRIORITY JOURNAL; PROTEIN STRUCTURE; PSEUDOMONAS PUTIDA; RADIOACTIVITY; BINDING SITE; CHEMICAL STRUCTURE; CHEMISTRY; ENZYMOLOGY; GENETICS; METABOLISM; PROTEIN TERTIARY STRUCTURE;

ESCHERICHIA COLI; PSEUDOMONAS PUTIDA;

BACTERIAL PROTEINS; BINDING SITES; CARBOXYLIC ESTER HYDROLASES; CATALYTIC DOMAIN; ESCHERICHIA COLI; MODELS, MOLECULAR; PROTEIN STRUCTURE, TERTIARY; PSEUDOMONAS PUTIDA; RECOMBINANT PROTEINS;

EID: 33947538844     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M608119200     Document Type: Article

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