Growth factor withdrawal in combination with sodium butyrate addition extends culture longevity and enhances antibody production in CHO cells

Journal of Biotechnology

Volumn 155, Issue 2, 2011, Pages 225-231

  Hong, Jong Kwang ^a,b   Lee, Gyun Min ^a   Yoon, Sung Kwan ^b  

^a Korea Advanced Institute of Science and Technology (KAIST)   (South Korea)

^b Bio R and D Center   (South Korea)

Author keywords

Bcl 2 expression; C Myc expression; CHO cells; Growth factor; Pyruvate dehydrogenase activity; Sodium butyrate

Indexed keywords

BCL-2 EXPRESSION; C-MYC EXPRESSION; CHO CELL; GROWTH FACTOR; PYRUVATES; SODIUM BUTYRATE;

ANTIBODIES; BOTTLES; CELL DEATH; CELL GROWTH; GROWTH KINETICS; PEPTIDES; SODIUM; VOLATILE FATTY ACIDS;

CELL CULTURE;

ANTIBODY; BUTYRIC ACID; GROWTH FACTOR; MYC PROTEIN; PROTEIN BCL 2; PYRUVATE DEHYDROGENASE;

ANIMAL CELL; ANTIBODY PRODUCTION; APOPTOSIS; ARTICLE; CELL CULTURE; CELL DEATH; CELL GROWTH; CELL VIABILITY; CHO CELL; FEMALE; GROWTH INHIBITION; LONGEVITY; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; TREATMENT WITHDRAWAL;

ANIMALS; ANTIBODY FORMATION; BLOTTING, WESTERN; BUTYRATES; CELL CULTURE TECHNIQUES; CELL PROLIFERATION; CHO CELLS; CRICETINAE; CRICETULUS; CULTURE MEDIA; DOSE-RESPONSE RELATIONSHIP, DRUG; INTERCELLULAR SIGNALING PEPTIDES AND PROTEINS; PROTO-ONCOGENE PROTEINS C-BCL-2; PROTO-ONCOGENE PROTEINS C-MYC; PYRUVATE DEHYDROGENASE (LIPOAMIDE);

CRICETULUS GRISEUS;

EID: 80051471697     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2011.06.020     Document Type: Article

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