Detection of Blastocystis homings in unpreserved stool specimens by using polymerase chain reaction

Journal of Parasitology

Volumn 92, Issue 5, 2006, Pages 1081-1087

  Stensvold, Rune ^a   Brillowska Dabrowska, Anna ^a   Nielsen, Henrik Vedel ^a   Arendrup, Maiken Cavling ^a  

^a STATENS SERUM INSTITUT   (Denmark)

Author keywords

[No Author keywords available]

Indexed keywords

FORMALDEHYDE; FORMOL ETHYL ACETATE; RIBOSOME DNA; UNCLASSIFIED DRUG; PROTOZOAL DNA;

DISEASE PREVALENCE; MORPHOLOGY; PARASITE; PARASITIC DISEASE; POLYMERASE CHAIN REACTION;

ARTICLE; BLASTOCYSTIS HOMINIS; CULTURE TECHNIQUE; DNA DETERMINATION; FECES ANALYSIS; FORMOL ETHYL ACETATE CONCENTRATION TECHNIQUE; GASTROINTESTINAL SYMPTOM; INTESTINE INFECTION; NONHUMAN; NUCLEOTIDE SEQUENCE; PATHOGENESIS; POLYMERASE CHAIN REACTION; PREVALENCE; SENSITIVITY ANALYSIS; AMEBIASIS; ANIMAL; CLUSTER ANALYSIS; EVALUATION; FECES; GENETICS; HUMAN; ISOLATION AND PURIFICATION; MOLECULAR GENETICS; PARASITOLOGY; PRESERVATION; SENSITIVITY AND SPECIFICITY; SEQUENCE ALIGNMENT; STANDARD;

BLASTOCYSTIS; BLASTOCYSTIS HOMINIS;

ANIMALS; BASE SEQUENCE; BLASTOCYSTIS HOMINIS; BLASTOCYSTIS INFECTIONS; CLUSTER ANALYSIS; DNA, PROTOZOAN; FECES; HUMANS; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; PRESERVATION, BIOLOGICAL; SENSITIVITY AND SPECIFICITY; SEQUENCE ALIGNMENT;

EID: 33845274289     PISSN: 00223395     EISSN: None     Source Type: Journal    
DOI: 10.1645/GE-840R.1     Document Type: Article

References (30)

1. ABE, N. 2004. Molecular and phylogenetic analysis of Blastocystis isolates from various hosts. Veterinary Parasitology 120: 235-242.
2. _, Z. WU, AND H. YOSHHIKAWA. 2003. Zoonotic genotypes of Blastocystis hominis detected in cattle and pigs by PCR with diagnostic primers and restriction fragment length polymorphism analysis of the small subunit ribosomal RNA gene. Parasitology Research 90: 124-128.
3. AMAR, C. F., P. H. DEAR, S. PEDRAZA-DIAZ, N. LOOKER, E. LINNANE, AND J. MCLAUCHLIN. 2002. Sensitive PCR-restriction fragment length polymorphism assay for detection and genotyping of Giardia duodenalis in human feces. Journal of Clinical Microbiology 40: 446-452.
4. ARISUE, N., T. HASHIMOTO, AND H. YOSHIKAWA. 2003. Sequence heterogeneity of the small subunit ribosomal RNA genes among Blastocystis isolates. Parasitology 126: 1-9.
5. BÖHM-GLONING, B., J. KNOBLOCH, AND B. WALDERICH. 1997. Five subgroups of Blastocystis hominis isolates from symptomatic and asymptomatic patients revealed by restriction site analysis of PCR-amplified 16S-like rDNA. Tropical Medicine and International Health 2: 771-778.
6. CLARK, C. G. 1997. Extensive genetic diversity in Blastocystis hominis. Molecular and Biochemical Parasitology 87: 79-83.
7. GUY, R. A., C. XIAO, AND P. A. HORGEN. 2003. Real-Time PCR assay for detection and genotype differentiation of Giardia lamblia in stool specimens. Journal of Clinical Microbiology 42: 3317-3320.
8. HO, L. C., K. JEYASEELAN, AND M. SINGH. 2001. Use of the elongation factor- 1α gene in a polymerase chain reaction-based restriction-fragment- length polymorphism analysis of genetic heterogeneity among Blastocystis species. Molecular and Biochemical Parasitology 112: 287-291.
9. HOEVERS, J., P. HOLMAN, K. LOGAN, M. HOMMEL, R. ASHFORD, AND K. SNOWDEN. 2000. Restriction-fragment-length polymorphism analysis of small-subunit rRNA genes of Blastocystis hominis isolates from geographically diverse human hosts. Parasitology Research 86: 57-61.
10. JONES, W. R. 1946. The experimental infection of rats with Entamoeba histolytica; with a method for evaluating the anti-amoebic properties of new compounds. Annals of Tropical Medicine and Parasitology 40: 130-140.
11. KANEDA, Y., N. HORIKI, X. J. CHENG, Y. FUJITA, M. MARUYAMA, AND H. TACHIBANA. 2001. Ribodemes of Blastocystis hominis isolated in Japan. American Journal of Tropical Medicine and Hygiene 65: 393-396.
12. LIMOR, J. R., A. A. LAL, AND L. XIAO. 2002. Detection and differentiation of Cryptosporidium parasites that are pathogenic for humans by real-time PCR. Journal of Clinical Microbiology 40: 2335-2338.
13. MELHORN, H. 1988. Blastocystis hominis, Brumpt 1912 - are there different stages or species. Parasitology Research 74: 393-395.
14. NOËL, C., C. PEYRONNET, D. GERBOD, V. EDGCOMB, P. DELGADO-VISCOGLIOSI, M. L. SOGIN, M. CAPRON, E. VISCOGLIOSI, AND L. ZENNER. 2003. Phylogenetic analysis of Blastocystis isolates from different hosts based on the comparison of small-subunit rRNA gene sequences. Molecular and Biochemical Parasitology 126: 119-123.
15. PEEK, R., F. R. REEDEKER, AND T. VAN GOOL. 2004. Direct amplification and genotyping of Dientamoeba fragilis from human stool samples. Journal of Clinical Microbiology 42: 631-635.
16. RIVERA, W. L., AND M. A. TAN. 2005. Molecular characterization of Blastocystis isolates in the Philippines by riboprinting. Parasitology Research 96: 253-257.
17. SILBERMAN, J. D., M. L. SOGIN, D. D. LEIPE, AND C. G. CLARK. 1996. Human parasite finds taxonomic home. Nature 380: 398-398.
18. STARK, D., N. BEEBE, D. MARRIOTT, J. ELLIS, AND J. HARKNESS. 2005. Detection of Dientamoeba fragilis in fresh stool specimens using PCR. International Journal for Parasitology 35: 57-62.
19. STENZEL, D. J., AND P. F. L. BOREHAM. 1991. A cyst-like stage of Blastocystis hominis. International Journal for Parasitology 21: 613-615.
20. _, AND _. 1996. Blastocystis hominis revisited. Clinical Microbiology Reviews 9: 563-584.
21. SURESH, K., AND H. SMITH. 2004. Comparison of methods for detecting Blastocystis hominis. European Journal of Clinical Microbiology and Infectious Diseases 23: 509-511.
22. TAN, K. W. S. 2004. Blastocystis in humans and animals: New insights using modern methodologies. Veterinary Parasitology 126: 121-144.
23. _, M. SINGH, AND E. H. YAP. 2002. Recent advances in Blastocystis hominis research: Hot spots in terra incognita. International Journal for Parasitology 32: 789-804.
24. TERMMATHURAPOJ, S., S. LEELAYOOVA, P. AIMPUN, U. THATHAISONG, T. NIMMANON, P. TAAMASRI, AND M. MUNGTHIN. 2004. The usefulness of short-term in vitro cultivation for the detection and molecular study of Blastocystis hominis in stool specimens. Parasitology Research 93: 445-447.
25. TROLL, H., H. MARTI, AND N. WEISS. 1997. Simple differential detection of Entamoeba histolytica and Entamoeba dispar in fresh stool specimens by sodium acetate-acetic-acid-formalin concentration and PCR. Journal of Clinical Microbiology 35: 1701-1705.
26. VERWEIJ, J. J., R. A. BLANGE, K. TEMPLETON, J. SCHINKEL, E. A. BRIENEN, M. A. VAN ROOYEN, L. VAN LIESHOUT, AND A. M. POLDERMAN. 2004. Simultaneous detection of Entamoeba histolytica, Giardia lamblia, and Cryptosporidium parvum in fecal samples by using multiplex real-time PCR. Journal of Clinical Microbiology 42: 1220-1223.
27. YOSHIKAWA, H., N. ABE, M. IWASAWA, S. KITANO, I. NAGANO, Z. WU, AND Y. TAKAHASHI. 2000. Genomic analysis of Blastocystis hominis strains isolated from two long-term health care facilities. Journal of Clinical Microbiology 38: 1324-1330.
28. _, I. NAGANO, Z. WU, E. H. YAP, M. SINGH, AND Y. TAKAHASHI. 1998. Genomic polymorphism among Blastocystis hominis strains and development of subtype-specific diagnostic primers. Molecular and Cellular Probes 12: 153-159.
29. _, Z. WU, I. KIMATA, M. ISEKI, I. K. M. D. ALI, M. B. HOSSAIN, V. ZAMAN, R. HAQUE, AND Y. TAKAHASHI. 2004. Polymerase chain reaction-based genotype classification among human Blastocystis hominis populations isolated from different countries. Parasitology Research 92: 22-29.
30. ZIERDT, C. H. 1991. Blastocystis hominis - Past and future. Clinical Microbiology Reviews 4: 61-79.