Expressing the human proteome for affinity proteomics: Optimising expression of soluble protein domains and in vivo biotinylation

New Biotechnology

Volumn 29, Issue 5, 2012, Pages 515-525

  Keates, Tracy ^a   Cooper, Christopher D O ^a   Savitsky, Pavel ^a   Allerston, Charles K ^a   Phillips, Claire ^a   Hammarström, Martin ^b,c   Daga, Neha ^a   Berridge, Georgina ^a   Mahajan, Pravin ^a   Burgess Brown, Nicola A ^a   Müller, Susanne ^a   Gräslund, Susanne ^b,d   Gileadi, Opher ^a  

^a UNIVERSITY OF OXFORD   (United Kingdom)

^b KAROLINSKA INSTITUTE   (Sweden)

^c PFIZER INC   (United States)

^d UNIVERSITY OF TORONTO   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

AFFINITY REAGENTS; AFFINITY-PROTEOMICS; BIOTINYLATIONS; HIGH QUALITY; HUMAN PROTEINS; HUMAN PROTEOME; IN-VIVO; OPTIMISATIONS; PROTEIN DOMAINS; PROTEIN FAMILY; SIMILAR NUMBERS; SOLUBLE PROTEINS; STRUCTURAL GENOMICS; STRUCTURE DETERMINATION; TARGET PROTEINS; TEST CASE;

ACYLATION; MOLECULAR BIOLOGY; OPTIMIZATION; PURIFICATION;

PROTEINS;

PROTEOME;

AMINO ACID SEQUENCE; ARTICLE; BACULOVIRUS EXPRESSION SYSTEM; BIOTINYLATION; CRYSTAL STRUCTURE; CRYSTALLIZATION; ESCHERICHIA COLI; HUMAN; IN VIVO STUDY; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN MODIFICATION; PROTEIN PURIFICATION; PROTEIN STRUCTURE; PROTEIN SYNTHESIS; PROTEOMICS;

ANIMALS; BIOTIN; BIOTINYLATION; CHROMATOGRAPHY, AFFINITY; CRYSTALLIZATION; CULTURE MEDIA; GENES; HUMANS; MASS SPECTROMETRY; PLASMIDS; PROTEIN STRUCTURE, TERTIARY; PROTEOME; PROTEOMICS; SOLUBILITY;

EID: 84862024555     PISSN: 18716784     EISSN: 18764347     Source Type: Journal    
DOI: 10.1016/j.nbt.2011.10.007     Document Type: Article

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