The 1.7 Å crystal structure of the regulator of chromosome condensation (RCC1) reveals a seven-bladed propeller

Nature

Volumn 392, Issue 6671, 1998, Pages 97-101

  Renault, Louis ^a,c   Nassar, Nicolas ^a,d   Vetter, Ingrid ^e   Becker, Jörg ^a   Klebe, Christian ^a   Roth, Michel ^b   Wittinghofer, Alfred ^a  

^a Abteilung Strukturelle Biologie   (Germany)

^b INSTITUT DE BIOLOGIE STRUCTURALE   (France)

^c Inst de Biol Struct Jean P

^d NONE   (United States)

^e NONE

Author keywords

[No Author keywords available]

Indexed keywords

DNA; GUANINE NUCLEOTIDE BINDING PROTEIN; GUANINE NUCLEOTIDE EXCHANGE FACTOR; MESSENGER RNA PRECURSOR; CELL CYCLE PROTEIN; DNA BINDING PROTEIN; GUANOSINE DIPHOSPHATE; NUCLEAR PROTEIN; RCC1 PROTEIN, HUMAN; RECOMBINANT PROTEIN;

ANIMAL CELL; ARTICLE; CELL CYCLE G1 PHASE; CHROMOSOME CONDENSATION; CONTROLLED STUDY; CRYSTAL STRUCTURE; GENE MUTATION; HAMSTER; NONHUMAN; PRIORITY JOURNAL; REGULATOR GENE; TEMPERATURE SENSITIVE MUTANT; X RAY CRYSTALLOGRAPHY; AMINO ACID SEQUENCE; ANIMAL; CELL LINE; CHEMICAL STRUCTURE; CHEMISTRY; DROSOPHILA; GENETICS; HUMAN; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; PROTEIN CONFORMATION; SCHIZOSACCHAROMYCES; SEQUENCE HOMOLOGY;

AMINO ACID SEQUENCE; ANIMALS; CELL CYCLE PROTEINS; CELL LINE; CLONING, MOLECULAR; CRICETINAE; CRYSTALLOGRAPHY, X-RAY; DNA-BINDING PROTEINS; DROSOPHILA; GUANINE NUCLEOTIDE EXCHANGE FACTORS; GUANOSINE DIPHOSPHATE; HUMANS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; NUCLEAR PROTEINS; PROTEIN CONFORMATION; RECOMBINANT PROTEINS; SCHIZOSACCHAROMYCES; SEQUENCE HOMOLOGY, AMINO ACID;

EID: 0032485075     PISSN: 00280836     EISSN: None     Source Type: Journal    
DOI: 10.1038/32204     Document Type: Article

References (29)

1. Ohtsubo, M. et al. Isolation and characterization of the active cDNA of the human cell cycle gene (RCC1) involved in the regulation of onset of chromosome condensation. Genes Dev. 1, 585-593 (1987).
2. Seki, T., Hayashi, N. & Nishimoto, T. RCC1 in the Ran pathway. J. Bioehem. 120, 207-214 (1996).
3. Aebi, M., Clark, M. W., Vijayraghavan, U. & Abelson, J. A yeast mutant, PRP20, altered in mRNA metabolism and maintenance of the nuclear structure, is defective in a gene homologous to the human gene RCC1 which is involved in the control of chromosome condensation. J. Mol. Gen. Genet. 224, 72-80 (1990).
4. Kadowaki, T., Goldfarb, D., Spitz, L. M., Tartakoff, A. M. & Ohno, M. Regulation of RNA processing and transport by a nuclear guanine nucleotide release protein and members of the Ras superfamily, EMBO J. 12, 2929-2937 (1993).
5. Clark, K. L. & Sprague, G. F. Jr Yeast pheromone response pathway: characterization of a supressor that restores mating to receptorless mutants. Mol. Cell Biol. 9, 2682-2694 (1989).
6. Matsumoto, T. & Beach, D. Premature initiation of mitosis in yeast lacking RCC1 or an interacting GTPase. Cell 66, 347-360 (1991).
7. Sazer, S, & Nurse, P. A fission yeast RCCl-related protein is required for the mitosis to interphase transition. EMBO J. 13, 606-615 (1994).
8. Bischoff, F. R. & Ponstingl, H, Catalysis of guanine nucleotide exchange on Ran by the mitotic regulator RCC1. Nature 354, 80-82 (1991).
9. Klebe, C, Prinz, H., Wittinghofer, A. & Goody, R. S. The kinetic mechanism of Ran-nucleotide exchange catalysed by RCC1. Biochemistry 34, 12543-12552 (1995).
10. Lambright, D. G. er al. The 2.0 Å crystal structure of a heterotrimeric G protein. Nature 379, 311-319 (1996).
11. 2. Cell 83, 1047-1058 (1995).
12. Neer, E. J., Schmidt, C. J., Nambudripad, R. & Smith, T. F. The ancient regulatory-protein family of WD-repeat proteins. Nature 371, 297-300 (1994).
13. Matynia, A. et al. Isolation and characterization of fusion yeast sns mutants defective at the mitosis to interphase transition. Genetics (in the press).
14. Azuma, Y. et al. Conserved histidine residues of RCI are essential for nucleotide exchange on Ran. J. Biochem. 120, 82-91 (1996).
15. Meindl, A. et al A gene (RPGR) with humology ot the RCCl guanine nucleotide exchange factor is mutated in X-linked retinis pigmentosa (RP3), Nature Genet. 13, 35-42 (1996).
16. Roepman, R. et al. Positional cloning of th gene for X-linked retinitis pigmentosa 3: homology with the guanine-nucleotide-exchange factor RCCl. Hum. Mol. Genet. 5, 1035-1041 (1996).
17. Seabra, M. C., Brown, M. S. & Goldstein, J. L. Retinal degeneration in choroideremia: deficiency of rab geranylgeranyl transferase. Science 259, 377-381 (1993).
18. Rosa, J. L., Casaroli-Marano, R. P., Buckler, A. J., Vilaró, S, & Barbacid, M. P532, a giant protein related to the chromosome condensation regulator RCCl, stimulates guanine nucleotide exchange on ARF1 and Rab proteins. EMBO J, 15, 4262-4273 (1996).
19. Kawashima, T., Berthet-Colominss, C., Wulff, M., Cusack, S. & Leberman, R. The structure of the Escherichia coli EF-Tu-EF-Ts complex at 2.5 Å resolution. Nature 379, 511-518 (1996).
20. Wang, Y., Jiang, Y., Meyering-Voss, M., Sprinzl, M. & Sigler, P. B. Crystal structure of the EF-Tu.EF-Ts complex form Thermus thermophilus. Nature Struct. Biol 4, 650-656 (1997).
21. Otwinowski, Z. & Minor, W. in Data Collection and Processing (eds Sawyer, L., Isaacs, N. & Bailey, S.) 556-562 (SERC, Warrington, UK, 1993).
22. Kabsch, W. Automatic processing of rotation diffraction data from crystals of initially unknown symmetry and cell contents. J. Appl. Crystallogr, 26, 795-800 (1993).
23. Collaborative Computational Project, N.4. The CCP4 suite: programs for protein crystallography. Acta Crystallogr. D 50, 760-763 (1994).
24. Vellicux, F. M. D., Hunt, J. F., Roy, S. & Read, R. J. DEMON/ ANGEL: a suite of programs to carry out density modifications. J. Appl. Crystallogr, 28, 347-351 (1995).
25. Jones, T. A. & Kjeldgaard, M. Electron-density map interpretation. Methods Enzymol. 277, 173-208 (1997).
26. Brünger, A. T. X-PLOR Version 3.8 (We Univ., 1996).
27. Lamzin,V. S. & Wilson, K. S. Automated refinement of protein models. Acta Crystallogr. D 49,129-147 (1993).
28. Kraulis, P. J. Molscript: a program to produce both detailed and schematic plots of protein structures. J. Appl. Crystallogr. 24, 946-950 (1991).
29. Merritt, E. A., Murphy, M. E. P. Raster3D Version 2.0. A program for photorealistic molecular graphics. Acta Crystallogr. D50, 869 (1994).