Isolation and expansion of adult cardiac stem/progenitor cells in the form of cardiospheres from human cardiac biopsies and murine hearts

Methods in Molecular Biology

Volumn 879, Issue , 2012, Pages 327-338

  Chimenti, Isotta ^a   Gaetani, Roberto ^b   Barile, Lucio ^c   Forte, Elvira ^b   Ionta, Vittoria ^b   Angelini, Francesco ^a   Frati, Giacomo ^a   Messina, Elisa ^b   Giacomello, Alessandro ^a,b  

^a Department of Medical Surgical Sciences and Biotechnology   (Italy)

^b SAPIENZA UNIVERSITY OF ROME   (Italy)

^c UNIVERSITY OF MILANO BICOCCA   (Italy)

Author keywords

Adult stem cells; Cardiac regeneration; Cardiospheres; Explant derived cells

Indexed keywords

ANIMAL; ARTICLE; BIOPSY; CELL PROLIFERATION; CELL SEPARATION; CYTOLOGY; HEART; HEART MUSCLE; HEART MUSCLE CELL; HUMAN; MOUSE; STEM CELL;

ANIMALS; BIOPSY; CELL PROLIFERATION; CELL SEPARATION; HEART; HUMANS; MICE; MYOCARDIUM; MYOCYTES, CARDIAC; STEM CELLS;

MURINAE;

EID: 84861822380     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-815-3_19     Document Type: Article

References (38)

1. Kajstura J et al (1998) Myocyte proliferation in end-stage cardiac failure in humans. Proc Natl Acad Sci U S A 95:8801-8805
2. Anversa P, Kajstura J (1998) Ventricular myocytes are not terminally differentiated in the adult mammalian heart. Circ Res 83:1-14
3. Chimenti I et al (2011) Evidence for the existence of resident cardiac stem cells (Chapter 9). In: Cohen IS, Gaudette GR (eds) Regenerating the heart, 1st edn. Springer Science+Business Media, New York
4. Limana F et al (2007) Identification of myocardial and vascular precursor cells in human and mouse epicardium. Circ Res 101: 1255-1265
5. Ott HC (2006) The adult human heart as a source for stem cells: repair strategies with embryonic-like progenitor cells. Nat Clin Pract Cardiovasc Med 4:S27-S39
6. Pfister O et al (2005) CD31-but Not CD31 + cardiac side population cells exhibit functional cardiomyogenic differentiation. Circ Res 97:52-61
7. Laugwitz KL et al (2005) Postnatal isl1+ cardioblasts enter fully differentiated cardiomyocyte lineages. Nature 433:647-653
8. Messina E et al (2004) Isolation and expansion of adult cardiac stem cells from human and murine heart. Circ Res 95:911-921
9. Martin CM et al (2004) Persistent expression of the ATP-binding cassette transporter, Abcg2, identifies cardiac SP cells in the developing and adult heart. Dev Biol 265:262-275
10. Oh H et al (2003) Cardiac progenitor cells from adult myocardium: homing, differentiation, and fusion after infarction. Proc Natl Acad Sci U S A 100:12313-12318
11. Beltrami AP et al (2003) Adult cardiac stem cells are multipotent and support myocardial regeneration. Cell 114:763-776
12. Hierlihy AM et al (2002) The post-natal heart contains a myocardial stem cell population. FEBS Lett 530:239-243
13. Gaetani R et al (2009) New perspectives to repair a broken heart. Cardiovasc Hematol Agents Med Chem 7:91-107
14. Barile L et al (2007) Endogenous cardiac stem cells. Prog Cardiovasc Dis 50:31-48
15. Cai J et al (2004) In search of "stemness". Exp Hematol 32:585-598
16. Davis DR et al (2010) Isolation and expansion offunctionally-competent cardiac progenitor cells directly from heart biopsies. J Mol Cell Cardiol 49:312-321
17. Altomare C et al (2010) Caffeine-induced Ca(2+) signaling as an index of cardiac progenitor cells differentiation. Basic Res Cardiol 105:737-749
18. Smith RR et al (2007) Regenerative potential of cardiosphere-derived cells expanded from percutaneous endomyocardial biopsy specimens. Circulation 115:896-908
19. Davis DR et al (2009) Validation of the cardiosphere method to culture cardiac progenitor cells from myocardial tissue. PLoS One 4: e7195
20. Johnston PV et al (2009) Engraftment, differentiation, and functional benefits of autologous cardiosphere-derived cells in porcine ischemic cardiomyopathy. Circulation 120:1075-1083
21. Li TS et al (2010) Cardiospheres recapitulate a niche-like microenvironment rich in stemness and cell-matrix interactions, rationalizing their enhanced functional potency for myocardial repair. Stem Cells 28(11):2088-2098
22. Malliaras K et al (2010) Intramyocardial injection of autologous cardiospheres or cardiosphere-derived cells preserves function and minimizes adverse ventricular remodeling in pigs with heart failure post-myocardial infarction. AHA scientific session 2010. Circulation 122(21):A19030
23. Terrovitis J et al (2009) Noninvasive quantification and optimization of acute cell retention by in vivo positron emission tomography after intramyocardial cardiac-derived stem cell delivery. J Am Coll Cardiol 54:1619-1626
24. Lee ST et al (2011) Intramyocardial injection of autologous cardiospheres or cardiospherederived cells preserves function and minimizes adverse ventricular remodeling in pigs with heart failure post-myocardial infarction. J Am Coll Cardiol 57:455-465
25. Smith RR et al (2008) Unselected human cardiosphere-derived cells are functionally superior to c-Kit-or CD90-purified cardiospherederived cells. AHA scientific session 2008. Circulation 118:S420
26. Bearzi C et al (2007) Human cardiac stem cells. Proc Natl Acad Sci U S A 104:14068-14073
27. Bearzi C et al (2009) Identification of a coronary vascular progenitor cell in the human heart. Proc Natl Acad Sci U S A 106:15885-15890
28. Bearzi C et al (2005) Characterization and growth of human cardiac stem cells. AHA scientific session. Circulation 111(13):1720
29. Galvez BG et al (2008) Cardiac mesoangioblasts are committed, self-renewable progenitors, associated with small vessels of juvenile mouse ventricle. Cell Death Differ 15:1417-1428
30. Dellavalle A et al (2007) Pericytes of human skeletal muscle are myogenic precursors distinct from satellite cells. Nat Cell Biol 9: 255-267
31. Galvez BG et al (2009) Human cardiac mesoangioblasts isolated from hypertrophic cardiomyopathies are greatly reduced in proliferation and differentiation potency. Cardiovasc Res 83: 707-716
32. Morosetti R et al (2006) MyoD expression restores defective myogenic differentiation of human mesoangioblasts from inclusion-body myositis muscle. Proc Natl Acad Sci U S A 103: 16995-17000
33. Huang Y et al (2009) Kidney-derived stromal cells modulate dendritic and T cell responses. J Am Soc Nephrol 20:831-841
34. Puglisi MA et al (2008) Identification and characterization of a novel expandable adult stem/progenitor cell population in the human exocrine pancreas. J Endocrinol Invest 31: 563-572
35. Fierabracci A et al (2008) Identification of an adult stem/progenitor cell-like population in the human thyroid. J Endocrinol 198: 471-487
36. Chimenti I et al (2010) Relative roles of direct regeneration versus paracrine effects of human cardiosphere-derived cells transplanted into infarcted mice. Circ Res 106:971-980
37. Gaetani R et al (2009) Differentiation of human adult cardiac stem cells exposed to extremely low-frequency electromagneticfields. Cardiovasc Res 82:411-420
38. Terrovitis J et al (2008) Ectopic expression of the sodium-iodide symporter enables imaging of transplanted cardiac stem cells in vivo by single-photon emission computed tomography or positron emission tomography. J Am Coll Cardiol 52:1652-1660