Improving arachidonic acid accumulation in Mortierella alpina through B-group vitamin addition

Bioprocess and Biosystems Engineering

Volumn 35, Issue 5, 2012, Pages 683-688

  Zeng, Yan ^a   Ji, Xiao Jun ^a   Chang, Shu Mei ^a   Nie, Zhi Kui ^a   Huang, He ^a  

^a NANJING TECH UNIVERSITY   (China)

Author keywords

Arachidonic acid; B group vitamins; Biosynthesis; Enzyme activity; Mortierella alpina

Indexed keywords

ACETYL-COA; ARACHIDONIC ACIDS; COFACTORS; GLUCOSE-6-PHOSPHATE DEHYDROGENASE; KEY ENZYMES; MALIC ENZYMES; METABOLIC FLUX; MORTIERELLA ALPINA; SPECIFIC ACTIVITY;

BIOCHEMISTRY; ENZYME ACTIVITY; UNSATURATED FATTY ACIDS; VITAMINS;

BIOSYNTHESIS;

ACETYL COENZYME A; ADENOSINE TRIPHOSPHATE CITRATE LYASE; ARACHIDONIC ACID; GLUCOSE; GLUCOSE 6 PHOSPHATE DEHYDROGENASE; LINOLEIC ACID; LINOLENIC ACID; MALATE DEHYDROGENASE (DECARBOXYLATING); NICOTINAMIDE; OLEIC ACID; PALMITIC ACID; PANTOTHENIC ACID; PYRIDOXINE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; STEARIC ACID; THIAMINE; VITAMIN B GROUP;

ARTICLE; CARBON SOURCE; CELLULAR PARAMETERS; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; ENZYME ACTIVITY; FATTY ACID SYNTHESIS; FUNGAL BIOMASS; FUNGAL CELL; FUNGUS GROWTH; LIPID ANALYSIS; LIPID COMPOSITION; LIPID STORAGE; MORTIERELLA ALPINA; NONHUMAN; PRIORITY JOURNAL;

ADENOSINE TRIPHOSPHATE; ARACHIDONIC ACID; FUNGAL PROTEINS; GLUCOSEPHOSPHATE DEHYDROGENASE; MORTIERELLA; MULTIENZYME COMPLEXES; OXO-ACID-LYASES; VITAMIN B COMPLEX;

MORTIERELLA ALPINA;

EID: 84861481747     PISSN: 16157591     EISSN: 16157605     Source Type: Journal    
DOI: 10.1007/s00449-011-0648-2     Document Type: Article

References (26)

1. Sakuradani E, Shimizu S (2009) Single cell oil production by Mortierella alpina. J Biotechnol 144:31-36
2. Jin MJ, Huang H, Xiao AH, Gao Z, Liu X, Peng C (2009) Enhancing arachidonic acid production by Mortierella alpina ME-1 using improved mycelium aging technology. Bioproc Biosyst Eng 32:117-122
3. Eroshin VK, Satroutdinov AD, Dedyukhina EG, Chistyakova TI (2000) Arachidonic acid production by Mortierella alpina with growth-coupled lipid synthesis. Process Biochem 35:1171-1175 (Pubitemid 30463508)
4. Higashiyama K, Fujikowa S, Park EY, Shimizu S (2002) Production of arachidonic acid by Mortierella fungi. Biotechnol Bioproc Eng 7:252-262
5. Yuan C, Wang J, Shang Y, Gong G, Yao J, Yu Z (2002) Production of arachidonic acid by Mortierella alpina I49-N18. Food Technol Biotechnol 40:311-315 (Pubitemid 36039649)
6. Totani N, Yamaguchi A, Yawata M, Ueda T (2002) The role of morphology during growth of Mortierella alpina in arachidonic acid production. J Oleo Sci 51:531-538
7. Hwang BH, Kim JW, Park CS, Kim YS, Ryu YW (2005) High level production of arachidonic acid by fed-batch culture of Mortierella alpina using NH4OH as a nitrogen source and pH control. Biotechnol Lett 27:731-735
8. Sakuradani E, Ando A, Ogawa J, Shimizu S (2009) Improved production of various polyunsaturated fatty acids through filamentous fungus Mortierella alpina breeding. Appl Microbiol Biotechnol 84:1-10
9. Shinmen Y, Shimizu S, Akimoto K, Kawashima H, Yamada H (1989) Production of arachidonic acid by Mortierella fungi: selection of a potent producer and optimization of culture conditions for large-scale production. Appl Microbiol Biotechnol 31:11-16
10. Lu JM, Peng C, Ji XJ, You JY, Cong LL, Ouyang PK, Huang H (2011) Fermentation characteristics of Mortierella alpina in response to different nitrogen sources. Appl Biochem Biotechnol 164:979-990
11. Nancib A, Nancib N, Meziane-Cherif D, Boubendir A, Fick M, Boudrant J (2005) Joint effect of nitrogen sources and B vitamin supplementation of date juice on lactic acid production by Lactobacillus casei subsp. rhamnosus. Bioresour Technol 96:63-67 (Pubitemid 39201337)
12. Xu GQ, Chu J, Zhuang YP, Wang YH, Zhang SL (2008) Effects of vitamins on the lactic acid biosynthesis of Lactobacillus paracasei NERCB 0401. Biochem Eng J 38:189-197 (Pubitemid 350298930)
13. Li Y, Chen J, Lun SY, Rui XS (2001) Efficient pyruvate production by a multi-vitamin auxotroph of Torulopsis glabrata: key role and optimization of vitamin levels. Appl Microbiol Biotechnol 55:680-685 (Pubitemid 32661575)
14. Bizukojc M, Beata P, Stanislaw L (2007) Supplementation of the cultivation media with B-group vitamins enhances lovastatin biosynthesis by Aspergillus terreus. J Biotechnol 127:258-268 (Pubitemid 46107131)
15. Jin MJ, Huang H, Xiao AH, Zhang K, Liu X, Li S, Peng C (2008) A novel two-step fermentation process for improved arachidonic acid production by Mortierella alpina. Biotechnol Lett 30:1087-1091
16. Ren LJ, Huang H, Xiao AH, Lian M, Jin LJ, Ji XJ (2009) Enhanced docosahexaenoic acid production by reinforcing acetyl-CoA and NADPH supply in Schizochytrium sp. HX-308. Bioproc Biosyst Eng 32:837-843
17. Jang HD, Lin YY, Yang SS (2005) Effect of culture media and conditions on polyunsaturated fatty acids production by Mortierella alpina. Bioresour Technol 96:1633-1644
18. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248-254
19. Evans CT, Ratledge C (1985) Possible regulatory roles of ATP: citrate lyase, malic enzyme and AMP deaminase in lipid accumulation by Rhodosporidium toruloides CBS 14. Can J Microbiol 31:1000-1005 (Pubitemid 16185705)
20. Levy R (1979) Glucose-6-phosphate dehydrogenase. Adv Enzym. 48:97-192
21. Ratledge C (2004) Fatty acid biosynthesis in microorganisms being used for single cell oil production. Biochimie 86:807-815
22. Wynn JP, Hamid AA, Ratledge C (1999) The role of malic enzyme in the regulation of lipid accumulation in filamentous fungi. Microbiology 145:1911-1917 (Pubitemid 29396921)
23. Ratledge C, Wynn JP (2002) The biochemistry and molecular biology of lipid accumulation in oleaginous microorganisms. Adv Appl Microbiol 51:1-51 (Pubitemid 34984904)
24. Yokota A, Shimizu H, Terasawa Y, Takaoka N, Tomita F (1994) Pyruvic acid production by a lipoic acid auxotroph of Escherichia coli W1485. Appl Microbiol Biotechnol 41:638-646
25. Miyata R, Yonehara T (1996) Improvement of fermentative production of pyruvate from glucose by Torulopsis glavrata IFO 0005: Studies on pyruvate production by yeast (II). J Ferment Bioeng 82:475-479 (Pubitemid 27014889)
26. Ames SR, Sarma P, Elvehjem C (1947) Transaminase and pyridoxine deficiency. J Biol Chem 167:135-141