Single cell oil production by Mortierella alpina

Journal of Biotechnology

Volumn 144, Issue 1, 2009, Pages 31-36

  Sakuradani, Eiji ^a   Shimizu, Sakayu ^a  

^a KYOTO UNIVERSITY   (Japan)

Author keywords

Arachidonic acid; Mortierella alpina; Polyunsaturated fatty acid

Indexed keywords

ARACHIDONIC ACID; ARACHIDONIC ACIDS; FILAMENTOUS FUNGI; LARGE-SCALE PRODUCTION; METABOLIC ENGINEERING; MORTIERELLA ALPINA; MORTIERELLA ALPINA 1S-4; MUTANT STRAIN; POLYUNSATURATED FATTY ACID; POLYUNSATURATED FATTY ACIDS; SINGLE CELL OIL; TRIACYLGLYCEROLS;

BIOCHEMISTRY; CELL CULTURE; CELL MEMBRANES; FATTY ACIDS; GLYCEROL; MOLECULAR BIOLOGY;

ACIDS;

ARACHIDONIC ACID; DELTA 12 DESATURASE; DELTA5 DESATURASE; DIACYLGLYCEROL; ELONGASE; FATTY ACID DERIVATIVE; FUNGAL ENZYME; LINOLEOYL COENZYME A DESATURASE; MUTANT PROTEIN; N 3 DESATURASE; OIL; OMEGA 3 FATTY ACID; OMEGA 6 FATTY ACID; PALMITIC ACID; POLYUNSATURATED FATTY ACID; STEARIC ACID; TRIACYLGLYCEROL; UNCLASSIFIED DRUG;

BIOENGINEERING; BIOTRANSFORMATION; CELL VIABILITY; DERIVATIZATION; DNA MODIFICATION; ENZYME ACTIVATION; ENZYME ACTIVITY; ENZYME INHIBITION; FUNGAL CELL; FUNGAL GENE; FUNGAL METABOLISM; FUNGAL STRAIN; FUNGUS CULTURE; FUNGUS GROWTH; GENE DISRUPTION; LIPID COMPOSITION; LIPOGENESIS; MOLECULAR BIOLOGY; MORTIERELLA ALPINA; MUTAGENESIS; MYCELIUM; NONHUMAN; PRIORITY JOURNAL; REVIEW; RNA INTERFERENCE; SEQUENCE HOMOLOGY;

ARACHIDONIC ACID; GENETIC ENGINEERING; MORTIERELLA; MUTATION; OILS;

FUNGI; MORTIERELLA ALPINA;

EID: 70350217253     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2009.04.012     Document Type: Review

References (54)

1. Aggelis G. Selective uptake of fatty acids by the yeast Yarrowia lipolytica. Eur. J. Lipid Sci. Technol. 105 11 (2003) 651-655
2. Aggelis G., Papadiotis G., and Komaitis M. Microbial fatty acid specificity. Folia Microbiol. (Praha) 42 2 (1997) 117-120
3. Amano H., Shinmen Y., Akimoto K., Kawashima H., Amachi T., Shimizu S., and Yamada H. Chemotaxonomic significance of fatty acid composition in the genus Mortierella (Zygomycetes, Mortierellaceae). Micotaxonomy 94 2 (1992) 257-265
4. Ando A., Sakuradani E., Ogawa J., and Shimizu S. Construction of an Agrobacterium tumefaciens-mediated transformation system for arachidonic acid-producing fungus Mortierella alpina 1S-4. JSBBA Conference, The Japan Society for Bioscience, Biotechnology, and Agrochemistry. Tokyo (2007) 55
5. Ando A., Sakuradani E., Ogawa J., and Shimizu S. Improvement of a versatile transformation system with a carboxin resistant marker for arachidonic acid-producing fungus Mortierella alpina 1S-4. JSBBA Conference, The Japan Society for Bioscience, Biotechnology, and Agrochemistry. Nagoya (2008) 249
6. Certik M., and Shimizu S. Biosynthesis and regulation of microbial polyunsaturated fatty acid production. J. Biosci. Biotechnol. 87 1 (1999) 1-14
7. Certik M., Sakuradani E., and Shimizu S. Desaturase-defective fungal mutants: useful tools for the regulation and overproduction of polyunsaturated fatty acids. Trends Biotechnol. 16 12 (1998) 500-505
8. Higashiyama K., Yaguchi T., Akimoto K., Fujikawa S., and Shimizu S. Enhancement of arachidonic acid production by Mortierella alpina. J. Am. Oil Chem. Soc. 75 11 (1998) 1501-1505
9. Higashiyama K., Fujikawa S., Park E., and Shimizu S. Production of arachidonic acid by Mortierella fungi. Biotechnol. Bioprocess Eng. 7 5 (2002) 252-262
10. Jareonkitmongkol S., Kawashima H., Shimizu S., and Yamada H. Production of 5,8,11-cis-eicosatrienoic acid by a Δ12-desaturase-defective mutant of Mortierella alpina 1S-4. J. Am. Oil Chem. 69 9 (1992) 939-944
11. Jareonkitmongkol S., Kawashima H., Shirasaka N., Shimizu S., and Yamada H. Production of dihomo-γ-linolenic acid by a Δ5-desaturase-defective mutant of Mortierella alpina 1S-4. Appl. Environ. Microbiol. 58 7 (1992) 2196-2200
12. Jareonkitmongkol S., Shimizu S., and Yamada H. Fatty acid desaturation defective mutants of an arachidonic acid-producing fungus, Mortierella alpina 1S-4. J. Gen. Microbiol. 138 (1992) 997-1002
13. Jareonkitmongkol S., Kawashima H., and Shimizu S. Inhibitory effects of lignan compounds on the formation of arachidonic acid in a Δ5-desaturase-defective mutant of Mortierella alpina 1S-4. J. Ferment. Technol. 76 5 (1993) 406-407
14. Jareonkitmongkol S., Sakuradani E., and Shimizu S. A novel Δ5-desaturase-defective mutant of Mortierella alpina 1S-4 and its dihomo-γ-linolenic acid productivity. Appl. Environ. Microbiol. 59 12 (1993) 4300-4304
15. Jareonkitmongkol S., Shimizu S., and Yamada H. Occurrence of two nonmethylene-interrupted fatty acids in a Δ6-desaturase-defective mutant of the fungus Mortierella alpina 1S-4. Biochim. Biophys. Acta 1167 2 (1993) 137-141
16. Jareonkitmongkol S., Shimizu S., and Yamada H. Production of an eicosapentaenoic acid-containing oil by a Δ12 desaturase-defective mutant of Mortierella alpina 1S-4. J. Am. Oil Chem. Soc. 70 (1993) 119-123
17. Jareonkitmongkol S., Sakuradani E., and Shimizu S. Isolation and characterization of a Δ9-desaturation-defective mutant of an arachidonic acid-producing fungus, Mortierella alpina 1S-4. J. Am. Oil Chem. Soc. 79 10 (2002) 1021-1026
18. Jaroenkinomongkol S., Sakuradani E., and Shimizu S. Isolation and characterization of an ω3-desaturation-defective mutant of an arachidonic acid-producing fungus, Mortierella alpina 1S-4. Arch. Microbiol. 161 4 (1994) 316-319
19. Kamada N., Kawashima H., Sakuradani E., Akimoto K., Ogawa J., and Shimizu S. Production of 8,11-cis-eicosadienoic acid by a Δ5 and Δ12 desaturase-defective mutant derived from the arachidonic acid producing fungus Mortierella alpina 1S-4. J. Am. Oil Chem. Soc. 76 11 (1999) 1269-1274
20. Kawashima H., Nishihara M., Hirano Y., Kamada N., Akimoto K., Konishi K., and Shimizu S. Production of 5,8,11-eicosatrienoic acid (mead acid) by a Δ6 desaturation activity-enhanced mutant derived from a Δ12 desaturase-defective mutant of an arachidonic acid-producing fungus, Mortierella alpina 1S-4. Appl. Environ. Microbiol. 63 5 (1997) 1820-1825
21. Kawashima H., Sakuradani E., Kamada N., Akimoto K., Konishi K., Ogawa J., and Shimizu S. Production of 8,11,14, 17-cis-eicosatetraenoic acid (20:4ω3) by a Δ5 and Δ12 desaturase-defective mutant of an arachidonic acid-producing fungus Mortierella alpina 1S-4. J. Am. Oil Chem. Soc. 75 11 (1998) 1495-1500
22. Kawashima H., Akimoto K., Higashiyama K., Fujikawa S., and Shimizu S. Industrial production of dihomo-γ-linolenic acid by a Δ5 desaturase-defective mutant of Mortierella alpina 1S-4 fungus. J. Am. Oil Chem. Soc. 77 11 (2000) 1135-1138
23. Kyle D.J., Sicotte V.J., Singer J.J., and Reeb S.E. Bioproduction of docosahexaenoic acid (DHA) by microalgae. In: Kyle D.J., and Ratledge C. (Eds). Industrial Applications of Single Cell Oils (1992), AOCS Press, IL 287-300
24. Mackenzie D.A., Wongwathanarat P., Carter A.T., and Archer D.B. Isolation and use of a homologous histone H4 promoter and a ribosomal DNA region in a transformation vector for the oil-producing fungus Mortierella alpina. Appl. Environ. Microbiol. 66 11 (2000) 4655-4661
25. Moreton R.S. Modification of fatty acid composition of lipid accumulating yeasts with cyclopropene fatty acid desaturase inhibitors. Appl. Microbiol. Biotechnol. 22 1 (1985) 41-45
26. Nakahara T., Yokochi Y., Higashihara T., Tanaka S., Yaguchi T., and Honda D. Production of docosahexaenoic and docosapentaenoic acid by Schizochytrium sp. isolated from Yap Islands. J. Am. Oil Chem. Soc. 73 11 (1996) 1421-1426
27. Ratledge C. Microbial lipids: commercial realities or academic curiosities. In: Kyle D.J., and Ratledge C. (Eds). Industrial Applications of Single Cell Oils (1992), AOCS Press, IL 1-15
28. Sakuradani E., Kamada N., Hirano Y., Nishihara M., Kawashima H., Akimoto K., Higashiyama K., Ogawa J., and Shimizu S. Production of 5,8,11-cis-eicosatrienoic acid by a Δ5 and Δ6 desaturation activity-enhanced mutant derived from a Δ12 desaturation activity-defective mutant of Mortierella alpina 1S-4. Appl. Microbiol. Biotechnol. 60 3 (2002) 281-287
29. Sakuradani E., Hirano Y., Kamada N., Nojiri M., Ogawa J., and Shimizu S. Improvement of arachidonic acid production by mutants with lowered ω3-desaturation activity derived from Mortierela alpina 1S-4. Appl. Microbiol. Biotechnol. 66 3 (2004) 243-248
30. Sakuradani E., Naka M., Kanamaru H., Ioka Y., Nojiri M., Ogawa J., and Shimizu S. Novel biosynthetic pathways for n-4 and n-7 polyunsaturated fatty acids in a mutant of an arachidonic acid-producing fungus, Mortierella alpina 1S-4. Abstracts of the 95th AOCS Annual Meeting and Expo, The American Oil Chemists' Society. Cincinnati, USA (2004) 22
31. Sakuradani E., Kimura T., Ogawa J., and Shimizu S. Accumulation of diacylglycerols by a mutant derived from an arachidonic acid-producing fungus, Mortierella alpina 1S-4. SBJ Annual Meeting, The Society for Biotechnology. Nagoya, Japan (2004) 155
32. Shimizu S., and Jareonkitmongkol S. Mortierella species (fungi): production of C20 polyunsaturated fatty acids. In: Bajaj Y.P.S. (Ed). Biotechnology in Agriculture and Forestry (Medical Plants VIII) vol. 33 (1995), Springer-Verlag, Berlin 308-325
33. Shimizu S., and Yamada H. Production of dietary and pharmacologically important polyunsaturated fatty acids by microbiological processes. Comments Agric. Food Chem. 2 3 (1990) 211-235
34. Shimizu S., Kawashima H., Akimoto K., Shinmen Y., and Yamada H. Microbial conversion of an oil containing α-linolenic acid to an oil containing eicosapentaenoic acid. J. Am. Oil Chem. Soc. 66 3 (1988) 342-347
35. Shimizu S., Akimoto K., Kawashima H., Shinmen Y., and Yamada H. Production of dihomo-γ-linolenic acid by Mortierella alpina 1S-4. J. Am. Oil Chem. Soc. 66 2 (1989) 237-244
36. Shimizu S., Kawashima H., Akimoto K., Shinmen Y., and Yamada H. Conversion of linseed oil to an eicosapentaenoic acid-containing oil by Mortierella alpina 1S-4 at low temperature. Appl. Microbiol. Biotechnol. 32 1 (1989) 1-4
37. Shimizu S., Akimoto K., Shinmen Y., Kawashima H., Sugano H., and Yamada H. Sesamin is a potent inhibitor of Δ5 desaturase in polyunsaturated fatty acid biosynthesis. Lipids 26 7 (1991) 512-516
38. Shimizu S., Jareonkitmongkol S., Kawashima H., Akimoto K., and Yamada H. Production of a novel ω1-eicosapentaenoic acid by Mortierella alpina 1S-4 grown on 1-hexadecene. Arch. Microbiol. 156 3 (1991) 163-166
39. Shimizu S., Kawashima H., Akimoto K., Shinmen Y., and Yamada H. Production of odd chain polyunsaturated fatty acids by Mortierella fungi. J. Am. Oil Chem. Soc. 68 4 (1991) 254-258
40. Shimizu S., Ogawa J., Kataoka M., and Kobayashi M. Screening of novel microbial enzymes for the production of biologically and chemically useful compounds. In: Schepter T. (Ed). Advances in Biochemical Engineering/Biotechnology vol. 58 (1997), Springer-Verlag, Berlin 45-87
41. Shimizu S., Ogawa J., and Sakuradani E. Metabolic engineering for oleaginous microorganisms. JSBBA Conference, The Japan Society for Bioscience, Biotechnology, and Agrochemistry. Tokyo (2003) 371
42. Shimizu S., Sakuradani E., and Ogawa J. Production of functional lipids by microorganisms: arachidonic acid and related polyunsaturated fatty acids, and conjugated fatty acids. Oleoscience 3 3 (2003) 129-139
43. Singh A., and Ward O.P. Microbial production of docosahexaenoic acid (DHA, C22:6). Adv. Appl. Microbiol. 45 (1997) 271-312
44. Suzuki O., Yokochi T., and Yamashina T. Studies on production of lipids in fungi (II). Lipid compositions of six species of Mucorales in Zygomycetes. J. Jpn. Oil Chem. Soc. 30 (1981) 863-868
45. Takeno S., Sakuradani E., Murata S., Inohara-Ochiai M., Kawashima H., Ashikari T., and Shimizu S. Cloning and sequencing of the ura3 and ura5 genes, and isolation and characterization of uracil auxotrophs of the fungus Mortierella alpina 1S-4. Biosci. Biotechnol. Biochem. 68 2 (2004) 277-285
46. Takeno S., Sakuradani E., Murata S., Inohara-Ochiai M., Kawashima H., Ashikari T., and Shimizu S. Establishment of an overall transformation system for an oil-producing filamentous fungus, Mortierella alpina 1S-4. Appl. Microbiol. Biotechnol. 65 4 (2004) 419-425
47. Takeno S., Sakuradani E., Tomi A., Inohara-Ochiai M., Kawashima H., Ashikari T., and Shimizu S. Improvement of the fatty acid composition of an oil-producing filamentous fungus, Mortierella alpina 1S-4, through RNA interference with Δ12-desaturase gene expression. Appl. Environ. Microbiol. 71 9 (2005) 5124-5128
48. Takeno S., Sakuradani E., Murata S., Inohara-Ochiai M., Kawashima H., Ashikari T., and Shimizu S. Molecular evidence that the rate-limiting step for the biosynthesis of arachidonic acid in Mortierella alpina is at the level of an elongase. Lipids 40 1 (2005) 25-30
49. Takeno S., Sakuradani E., Tomi A., Inohara-Ochiai M., Kawashima H., and Shimizu S. Transformation of oil-producing fungus, Mortierella alpina 1S-4, using Zeocin, and application to arachidonic acid production. J. Biosci. Bioeng. 100 6 (2005) 617-622
50. Totani N., and Oba A. The filamentous fungus Mortierella alpina, high in arachidonic acid. Lipids 22 12 (1987) 1060-1062
51. Wynn J.P., and Ratledge C. Evidence that the rate-limiting step for the biosynthesis of arachidonic acid in Mortierella alpina is at the level of the 18:3 to 20:3 elongase. Microbiology 146 9 (2000) 2325-2331
52. Yamada H., Shimizu S., and Shinmen Y. Production of arachidonic acid by Mortierell elongata 1S-5. Agric. Biol. Chem. 51 3 (1987) 785-790
53. Yamada H., Shimizu S., Shinmen Y., Akimoto K., Kawashima H., and Jareonkitmongkol S. Production of dihomo-γ-linolenic acid, arachidonic acid, and eicosapentaenoic acid by filamentous fungi. In: Kyle D.J., and Ratledge C. (Eds). Industrial Applications of Single Cell Oils (1992), AOCS Press, IL 118-138
54. Yazawa K., Watanabe K., Ishikawa C., Kondo K., and Kimura S. Production of eicosapentaenoic acid from marine bacteria. In: Kyle D.J., and Ratledge C. (Eds). Industrial Applications of Single Cell Oils (1992), AOCS Press, IL 29-51